Bioremediation (bioaugmentation/biostimulation) trials of oil polluted seawater: A mesocosm simulation study

Hassanshahian, Mehdi; Emtiazi, Giti; Caruso, Gabriella; Cappello, Simone

doi:10.1016/j.marenvres.2013.12.010

Cited by 111 publications

(58 citation statements)

References 38 publications

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“…This observation indicates that the use of slow release inorganic NPK fertilizer enhanced the TPH reduction in crude oil-contaminated marine water. Similar observations have been reported for the use of inorganic fertilizer (nutrients) of different types such as nitrogen/nitrates and phosphorus (Nikolopoulou et al, 2013), NPK fertilizer (Cappelo et al, 2015;Hamzah et al, 2016;Kumari et al, 2016) and urea fertilizer (Chikere et al, 2016) in the bioremediation of soil and sediment contaminated with petroleum hydrocarbon/crude oil as well as the use of inorganic nutrient (Hassanshahian et al, 2014) for the biodegradation of crude oil in sea water. Figure 1 (c) shows the effects of commercial activated carbon (factor C) on percent TPH reduction.…”

Section: % Tph Reductionsupporting

confidence: 71%

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Statistical optimization and kinetic studies of enhanced bioremediation of crude oil - contaminated marine water using combined adsorption-biostimulation strategy

Agarry¹

2017

Journal of Applied Sciences and Environmental Management

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ABSTRACT:The objectives of this study were to investigate and evaluate through Taguchi orthogonal experimental design the effects of amendment agents as well as the optimization and kinetics of enhanced bioremediation of crude oil-contaminated marine water using combined adsorption-biostimulation strategy. The amendment agents are organic fertilizer, slow release inorganic NPK fertilizer, commercial activated carbon and Tween 80. Bioremediation of weathered Escravos crude oil (WECO)-contaminated marine water was studied for four weeks in ten plastic buckets open system. The oil-contaminated marine water in nine of the buckets was each amended with the amendment agents according to Taguchi's (L9) orthogonal array of four factors and three levels and the tenth un-amended bucket served as control (natural bioattenuation). Results showed that the amendment agents had relative significant influence on the enhancement of WECO-marine water bioremediation. The optimum amendment agent conditions were determined as follows: organic fertilizer 30 g, inorganic slow release NPK fertilizer 4 g, activated carbon 30 g, and Tween 80, 1.5 g. Under these optimum conditions, the optimum percentage of crude oil biodegradation achieved was 98.25%. Bioremediation kinetic data of WECO fitted well to the first-order kinetic model. The combined adsorptionbiostimulation strategy (using the amendment agents) resulted in higher biodegradation rate constant, k (0.0443 -0.1183 day -1 ) and lower biodegradation half-life,

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Section: % Tph Reductionsupporting

confidence: 71%

“…Few workers have reported the positive effective use of inorganic nutrient (Hassanshahian et al, 2014), slow release fertilizer (Gertler et al, 2009), guano organic fertilizer that contains uric acid (Knezevich et al, 2007), cow dung (Umanu et al, 2013) and agro-industrial solid waste date (Elmahdi et al, 2014) …”

mentioning

confidence: 99%

Statistical optimization and kinetic studies of enhanced bioremediation of crude oil - contaminated marine water using combined adsorption-biostimulation strategy

Agarry¹

2017

Journal of Applied Sciences and Environmental Management

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“…On the other hand, a lesser number of cells can be counted if bacteria are distributed more uniformly on the glass slide. Most of the previous studies did not report the number of bacterial cells counted [5,14,49,[64][65][66][67]. Some studies reported that a minimum of 400-1000 bacterial cells were counted in a sample [57,58,61,62].…”

Section: Accuracy and Reliability Of The Arithmetic Meanmentioning

confidence: 99%

Evaluating the Reliability of Counting Bacteria Using Epifluorescence Microscopy

Muthukrishnan

Govender

Dobretsov

et al. 2017

JMSE

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Abstract:The common practice of counting bacteria using epifluorescence microscopy involves selecting 5-30 random fields of view on a glass slide to calculate the arithmetic mean which is then used to estimate the total bacterial abundance. However, not much is known about the accuracy of the arithmetic mean when it is calculated by selecting random fields of view and its effect on the overall abundance. The aim of this study is to evaluate the accuracy and reliability of the arithmetic mean by estimating total bacterial abundance and to calculate its variance using a bootstrapping technique. Three fixed suspensions obtained from a three-week-old marine biofilm were stained and dispersed on glass slides. Bacterial cells were counted from a total of 13,924 fields of view on each slide. Total bacterial count data obtained were used for calculating the arithmetic mean and associated variance and bias for sample field sizes of 5, 10, 15, 20, 25, 30, 35 and 40. The study revealed a non-uniform distribution of bacterial cells on the glass slide. A minimum of 20 random fields of view or a minimum of 350 bacterial cells need to be counted to obtain a reliable value of the arithmetic mean to estimate the total bacterial abundance for a marine biofilm sample dispersed on a glass slide.

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“…It has been reported that CHUB, which originally were able to degrade some crude oil fractions (petrol and engine oil) on separate occasions were unable to do so when both fractions were mixed (Shankar et al 2014). More so, it has been reported that single bacteria species tended to exhibit limited hydrocarbon degradation compared to consortia, owing to wide system of degradative enzyme in the latter (Hassanshahian et al 2014;Nkem et al 2016). It is not surprising that isolates from control sample were positive for hydrocarbon degradation.…”

Section: Discussionmentioning

confidence: 99%

Shift in microbial group during remediation by enhanced natural attenuation (RENA) of a crude oil-impacted soil: a case study of Ikarama Community, Bayelsa, Nigeria

2017

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Acute and chronic pollution of environments with crude oil does not bode well for biota living within the vicinity of polluted environments. This is due to environmental and public health concerns on the negative impact of crude oil pollution on living organisms. Enhancing microbial activities by adding nutrients and other amendments had proved effective in pollutant removal during bioremediation. This study was carried out to determine how microbial group respond during remediation by enhanced natural attenuation (RENA) during a field-scale bioremediation. Crude oil-polluted soil samples were collected (before, during, and after remediation) from a site undergoing remediation by enhanced natural attenuation (RENA) at Ikarama Community, Bayelsa State, Nigeria, and were analyzed for total petroleum hydrocarbon (TPH), polyaromatic hydrocarbon (PAH), and a shift in microbial community. The gas chromatography-flame ionization detector (GC-FID) results showed that the pollutant concentrations (TPH and PAH) reduced by 98 and 85%, respectively, after the remediation. Culturable hydrocarbon utilizing bacteria (CHUB) was highest (8.3 9 10 4 cfu/g) for sample collected during the remediation studies, whilst sample collected after remediation had low CHUB (6

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Bioremediation (bioaugmentation/biostimulation) trials of oil polluted seawater: A mesocosm simulation study

Cited by 111 publications

References 38 publications

Statistical optimization and kinetic studies of enhanced bioremediation of crude oil - contaminated marine water using combined adsorption-biostimulation strategy

Statistical optimization and kinetic studies of enhanced bioremediation of crude oil - contaminated marine water using combined adsorption-biostimulation strategy

Evaluating the Reliability of Counting Bacteria Using Epifluorescence Microscopy

Shift in microbial group during remediation by enhanced natural attenuation (RENA) of a crude oil-impacted soil: a case study of Ikarama Community, Bayelsa, Nigeria

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