Bioremediation and biocontrol of commercial probiotic in marine shrimp culture with biofloc

Ferreira, Maria Gabriela P.; Melo, Fabiana Penalva de; Lima, João Paulo Viana de; Andrade, Humber Agrelli; Severi, William; Correia, Eudes de Souza

doi:10.3856/vol45-issue1-fulltext-16

Cited by 35 publications

(17 citation statements)

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“…By using biofloc technology, intensive shrimp culture can be carried out with reduced water usage, pathogen free and reduced waste output 23 , 24 . In shrimp, biofloc rearing has been extensively standardized with P. vannamei in terms of growth, water quality and health 8 , 9 , 13 , 25 – 27 . This technology can be popularized further by adopting it to other species of shrimps.…”

Section: Resultsmentioning

confidence: 99%

Bioaugmentation of biofloc system with enzymatic bacterial strains for high health and production performance of Penaeus indicus

Panigrahi

Esakkiraj

Das

et al. 2021

Sci Rep

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The beneficial effects of two probiotic bacterial strains Marinilactibacillus piezotolerans and Novosphingobium sp. during the culture of Indian white shrimp, Penaeus indicus, under biofloc and clear water system were evaluated. The experimental variation were CW1 (M. piezotolerans in clear water), BFT1 (biofloc + M. piezotolerans), CW2 (Novosphingobium sp. in clear water), BFT2 (biofloc + Novosphingobium sp.) and control (without bacterial strains and biofloc). Growth and survival considerably increased in probiotic bio-augmented treatments. Probiotic incorporation significantly improved water quality, especially ammonia reduction. Microbiota analysis from gut samples taken from different treatments revealed varied microbial population structure among clear water culture, biofloc culture and control. Proteobacteria and Firmicutes were the top phyla observed in the treatments which were significantly higher in bio-augmented systems than the control. Vibrio genera were predominantly observed in control and clear water system compared to that of biofloc systems. Immune genes were significantly altered in response to probiotic gut microbial supplementation than the control. Higher gene expression profile of important immune genes was observed in the biofloc reared shrimps. Expression of digestive enzyme related genes such as trypsin, chymotrypsin, cathepsin L, cathepsin B and alpha amylase were also upregulated significantly in probiotic supplementation especially in the biofloc treatments. Proteomic analysis of hepatopancreas of shrimps from different treatments was carried out by using 2D gel electrophoresis and MALDI-TOF analysis. The proteins were mostly related to growth and stress tolerance. Eukaryotic initiation factor 4E binding protein was expressed in all the groups and it was high in biofloc treated animals followed by animals treated solely with probiotics compared to those of control groups. The results concludes that biofloc already proved as an effective culture method for healthy shrimp production and supplementation of probiotic bacterial strains registered additional benefit for growth, survival, microbial, immunological status of P, indicus culture.

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Section: Resultsmentioning

confidence: 99%

Bioaugmentation of biofloc system with enzymatic bacterial strains for high health and production performance of Penaeus indicus

Panigrahi

Esakkiraj

Das

et al. 2021

Sci Rep

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“…This indicates that high C/N ratio enhanced the richness of beneficial microbes whose bioactive metabolites aided in suppressing the harmful pathogens. The bioflocs in L. vannamei culture in a study was reported for the presence Bacillus sp., which demonstrates the probiotic nature of biofloc and also its ability to act as a biocontrol agent in water from super intensive aquaculture systems (Ferreira et al 2017).…”

Section: Effect Of Biofloc System On Shrimp and Aquaculture Microbiomementioning

confidence: 87%

“…The bioflocs in L. vannamei culture in a study was reported for the presence Bacillus sp., which demonstrates the probiotic nature of biofloc and also its ability to act as a biocontrol agent in water from super intensive aquaculture systems (Ferreira et al . 2017).…”

Section: Effect Of Biofloc System On Shrimp and Aquaculture Microbiomementioning

confidence: 99%

Healthy microbiome: a key to successful and sustainable shrimp aquaculture

Rajeev

Adithya

Kiran

et al. 2020

Reviews in Aquaculture

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The microbiome of the host and its surroundings play a vital role in its health and well-being. Unlike the terrestrial organisms that maternally acquire its microbiome, the aquatic species mainly get colonized by the microorganisms in its aquatic surrounding and are exposed to a wide variety of microbial community based on the type of aquatic environment the host inhabits. This review is focused on the aquaculture and shrimp microbiome and factors that influence its microbiome such as prebiotics, probiotics, synbiotics, diet, stress conditions, frequency and type of antibiotics used, host physiological conditions and developmental stages. This review also casts light on the role of the microbiome in enhancing the shrimp health, immune response, microbial colonization, and the effects of the biofloc system on shrimp and aquaculture microbiome. Moreover, beneficial microbes can be a safer alternative to harmful antibiotics used in aquaculture, which can thereby prevent the emergence of superbugs and also enhance the production rate. A proper understanding of the interactions between the host and its microbiome can help us to modulate and manipulate the aquaculture microbiome to enhance the health of aquatic organisms, which ensures the growth of the aquaculture industry. Therefore, the treatment strategies should be targeted towards the modulation of shrimp and aquaculture microbiome, which acts as beneficial partners to overcome the challenges confronted by the aquaculture sector.

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“…Microalgae are abundant in the natural environment and coexist with a wide range of microorganisms ( Fuentes et al, 2016 ; Berthold et al, 2019 ). Symbiotic relationships between algae and microorganisms play important roles in natural ecosystems ( Silva-Benavides & Torzillo, 2012 ), and have been well exploited in biofloc technology to improve the performance of aquaculture species (through reduction of the feed conversion ratio), wastewater treatment, and prevention of disease outbreaks ( De Souza et al, 2014 ; Long et al, 2015 ; Ferreira et al, 2017 ; Wang et al, 2019 ; Nurarina et al, 2020 ). While studies have demonstrated the importance of different forms of bacterial/microalgae isolated from diverse environments ( Gonzalez & Bashan, 2000 ; Mayali & Doucette, 2002 ; Kim et al, 2014 ; Fuentes et al, 2016 ; Toyama et al, 2018 ), only a few have reported the symbiotic effect of co-culturing floc-forming bacteria with microalgae dominant in biofloc ( Wang et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Marine microalgae co-cultured with floc-forming bacterium: Insight into growth and lipid productivity

Yee

Okomoda

Hashim

et al. 2021

PeerJ

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This study investigated the effect of co-culturing microalgae with a floc-forming bacterium. Of the six microalgae isolated from a biofloc sample, only Thalassiosira weissflogii, Chlamydomonas sp. and Chlorella vulgaris were propagated successfully in Conway medium. Hence, these species were selected for the experiment comparing microalgae axenic culture and co-culture with the floc-forming bacterium, Bacillus infantis. Results obtained showed that the co-culture had higher microalgae biomass compared to the axenic culture. A similar trend was also observed concerning the lipid content of the microalgae-bacterium co-cultures. The cell number of B. infantis co-cultured with T. weissflogii increased during the exponential stage until the sixth day, but the other microalgae species experienced a significant early reduction in cell density of the bacteria at the exponential stage. This study represents the first attempt at co-culturing microalgae with B. infantis, a floc-forming bacterium, and observed increased biomass growth and lipid accumulation compared to the axenic culture.

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Bioremediation and biocontrol of commercial probiotic in marine shrimp culture with biofloc

Cited by 35 publications

References 0 publications

Bioaugmentation of biofloc system with enzymatic bacterial strains for high health and production performance of Penaeus indicus

Bioaugmentation of biofloc system with enzymatic bacterial strains for high health and production performance of Penaeus indicus

Healthy microbiome: a key to successful and sustainable shrimp aquaculture

Marine microalgae co-cultured with floc-forming bacterium: Insight into growth and lipid productivity

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