Bioremediase a unique protein from a novel bacterium BKH1, ushering a new hope in concrete technology

Biswas, Mukul; Majumdar, Sudipta; Chowdhury, Trinath; Chattopadhyay, Brajadulal; Mandal, Saroj; Halder, Umesh Chandra; Yamasaki, Shinji

doi:10.1016/j.enzmictec.2010.03.005

Cited by 46 publications

(40 citation statements)

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“…Similar increment of tensile strength (>20%) of the mortar samples was also observed by incorporating this bacterium (data not supplied). The bacterium was found to secrete bioremediase-like protein which similarly possessed the property of biosilicification as found in BKH1 and BKH2 bacterial species described earlier [13] [15]. It might, therefore, be inferred that the bioremediase like protein similarly produced new Gehlenite phase within the pores of the mortar matrices which took active role in enhancement of compressive strength and other properties of as prepared bio-concrete material as elaborately described in the previous publications [12] [15] [16].…”

Section: Resultsmentioning

confidence: 78%

“…The bacteria BKH1 (99% homologous with Thermoanaerobactor thermohydrosulfuricus) and BKH2 (97% homologous with Cupriavidus metallidurans) both are found efficacious for enhancement of compressive strength and overall concrete properties [13] [15] [16]. These two bacteria when grown in a special semisynthetic medium, secrete extracellular proteins which when applied in concrete mixtures enhanced the strength of the concrete [13] [15]. In the present study, the 16S rRNA gene sequence methodology was employed to identify another bacterium from the same consortium and to test the same concrete/mortar specimen to observe its applicability on the improvement of its strength.…”

Section: Introductionmentioning

confidence: 99%

“…There are two bacterial strains (BKH1 & BKH2) already isolated and characterized from water of the 65˚C hot spring consortium of Bakreshwar, India. The bacteria BKH1 (99% homologous with Thermoanaerobactor thermohydrosulfuricus) and BKH2 (97% homologous with Cupriavidus metallidurans) both are found efficacious for enhancement of compressive strength and overall concrete properties [13] [15] [16]. These two bacteria when grown in a special semisynthetic medium, secrete extracellular proteins which when applied in concrete mixtures enhanced the strength of the concrete [13] [15].…”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic Characterization of BKH3 Bacterium Isolated from a Hot Spring Consortium of Bakreshwar (India) and Its Application

Chaudhuri¹,

Alam²,

Sarkar³

et al. 2016

AiM

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Background: The silica leaching attribute of some of the mystifying bacteria present in the cluster of hot springs (temperatures range 35˚C -80˚C) at Bakreshwar (West Bengal, India, 23˚52'48"N; 87˚22'40") has provided some significant advancements in construction technology when incorporated to the concrete/mortar specimens. The present investigation was designed to isolate other novel bacterial strains from 65˚C hot spring that could have similar or better performance in construction technology. Methods: Soil sample collected from the 65˚C hot spring was inoculated to the culture vials (incubated at 65˚C) containing a specific synthetic growth medium (pH 8.0) to grow the bacterial population anaerobically by degassing the medium with CO2 gas. Subsequent serial dilution techniques were employed to isolate pure culture of a specific bacterial strain. 16S rRNA gene sequence and phylogenetic analysis was carried out to identify the novelty of the isolated bacterial strain. The isolated bacteria were incorporated to the cement sand mixture at various cell concentrations to evaluate the efficacy of the strain in construction technology. Results: The work revealed the presence of a novel bacterial strain (BKH3; GenBank Accession No.: KP 890928) within the same hot spring consortium whose 16S rRNA gene sequence data showed 96% identity with Citrobacter freundii bacterial species. The newly isolated bacteria when incorporated at different cell concentration to the cement/cement-sand mixture were found to possess the similar compressive strength increment property, the cracks repairing ability and the water ingression resistivity. It also reduced the permeability of sulphate ions to the cementitious matrix reflecting the increment of durability of the incorporated material. Conclusions: The enhancement of compressive strength and durability of the as prepared bio-concrete material by using the isolated bacterial strain (BKH3) was due to the silica leaching activity of the bioremediase like pro-* Corresponding author. B. Chaudhuri et al. 454tein secreted by the bacterium. This may open up another vista of utilization of hot spring bacterium for beneficial purposes in construction technology.

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Section: Resultsmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phylogenetic Characterization of BKH3 Bacterium Isolated from a Hot Spring Consortium of Bakreshwar (India) and Its Application

Chaudhuri¹,

Alam²,

Sarkar³

et al. 2016

AiM

Self Cite

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“…The bacterial strain BKH1 (Gen Bank accession number FJ177512) was obtained from the Biophysics Laboratory, Department of Physics, Jadavpur University [13]. The analytical grade TEOS was purchased from the Merck, USA.…”

Section: Methodsmentioning

confidence: 99%

“…The protein was purified from 6 to 7 days old bacterial culture medium similarly as described by Biswas et al [13]. Double step purification through Sephadex G-100 column chromatographic technique was employed to purify the enzyme.…”

Section: Isolation and Purification Of The Bioremediase Proteinmentioning

confidence: 99%

A microbial protein-assisted silica ball comprising of silica-nanoparticles with plausible optical properties for multiple applications

Chattopadhyay¹

2017

SDRP-JNMS

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Background: Production of mesoporous silica nanoparticles and its conceivable applications in the fields of chromatography, surface polishing, catalysis and drug delivery etc. has gained momentum recently. We demonstrate here an efficient methodology for the amicable synthesis of silica balls consisting of mesoporous silica nanoparticles (SiO 2 -NPs) by using a secretary protein (bioremediase). The protein was isolated from a thermophilic non-pathogenic bacterium BKH1 (GenBank Accession No. FJ177512). Methods: Silica ball was formed at ambient temperature by mixing the dissolved bacterial protein dropwise to an organic precursor tetra-ethyl-orthosilicate (TEOS) solution at neutral pH environment. Surface morphology and compositional studies of prepared silica ball were carried out by using High-Resolution Transmission Electron Microscope (HRTEM) and Field Emission Scanning Electron Microscope equipped with Energy Dispersive X-ray Analyzer (FESEM-EDX). Fourier Transform Infrared Spectroscopy (FTIR) and X-ray diffraction (XRD) studies were further carried out for auxiliary characterization to determine the nature of SiO 2 -NPs. Stability of the as prepared SiO2-NPs was determined by noting the Zeta potential (ζ). The dye degradation activity of the silica balls was noted against different dyes. Results: Silica ball thus formed consisted of silica nanoparticles whose average dimensions were 20 ± 10 nm (n = 100). The size of the silica ball and also the sizes of the constituents' nanoparticles depend on the protein concentration in the reaction mixture. The result of zeta potential implied the moderate stability of SiO 2 -NPs at neutral pH environment. The SiO 2 -NPs showed green fluorescence emission which might have feasibly applications in the field of biomedical imaging. The decolourizing effect of silica ball on various dyes is a cost effective phenomenon as it can be used repeatedly. Conclusion:The pr otein-assisted silica balls preparation has a special consequence as it is an environmentally benign, lucrative and one pot synthesis approach which could be used repeatedly for various biomedical and chromatographic packing purposes.

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Exploring the Application and Prospects of Synthetic Biology in Engineered Living Materials

Wang,

Hu,

et al. 2023

Advanced Materials

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At the intersection of synthetic biology and materials science, engineered living materials (ELMs) exhibit unprecedented potential. Possessing unique “living” attributes, ELMs represent a significant paradigm shift in material design, showcasing self‐organization, self‐repair, adaptability, and evolvability, surpassing conventional synthetic materials. This review focuses on reviewing the applications of ELMs derived from bacteria, fungi, and plants in environmental remediation, eco‐friendly architecture, and sustainable energy. The review provides a comprehensive overview of the latest research progress and emerging design strategies for ELMs in various application fields from the perspectives of synthetic biology and materials science. In addition, the review provides valuable references for the design of novel ELMs, extending the potential applications of future ELMs. The investigation into the synergistic application possibilities amongst different species of ELMs offers beneficial reference information for researchers and practitioners in this field. Finally, future trends and development challenges of synthetic biology for ELMs in the coming years are discussed in detail.This article is protected by copyright. All rights reserved

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Bioremediase a unique protein from a novel bacterium BKH1, ushering a new hope in concrete technology

Cited by 46 publications

References 15 publications

Phylogenetic Characterization of BKH3 Bacterium Isolated from a Hot Spring Consortium of Bakreshwar (India) and Its Application

Phylogenetic Characterization of BKH3 Bacterium Isolated from a Hot Spring Consortium of Bakreshwar (India) and Its Application

A microbial protein-assisted silica ball comprising of silica-nanoparticles with plausible optical properties for multiple applications

Exploring the Application and Prospects of Synthetic Biology in Engineered Living Materials

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