Biorefinery production of poly-3-hydroxybutyrate using waste office paper hydrolysate as feedstock for microbial fermentation

Annamalai, Neelamegam; Al-Battashi, Huda; Al-Bahry, Saif N.; Sivakumar, Nallusamy

doi:10.1016/j.jbiotec.2017.11.002

Cited by 55 publications

(14 citation statements)

References 32 publications

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“…In the present study, it was observed that after the first cyclic event, a copolymer containing 62.61% HB and 37.39% HV was elucidated, whereas after CFBF cycles 2-4, the HB-HV-HHx terpolymer was noted (Table 5 and Additional file 1: Figures S1-S7). This differs from previous reports, which state that PHB was the predominant biopolymer synthesized when agrowaste-derived hydrolyzates from bagasse (Yu and Stahl 2008), wheat bran (Annamalai and Sivakumar 2016), rice straw (Sindhu et al 2016), wood (Wang and Liu 2014), wheat straw (Ferreira and Schlottbom 2016), and waste office paper (Annamalai et al 2017) were the sole carbon source in the fermentation.…”

Section: Polymer Compositioncontrasting

confidence: 99%

Optimization of cultivation medium and cyclic fed-batch fermentation strategy for enhanced polyhydroxyalkanoate production by Bacillus thuringiensis using a glucose-rich hydrolyzate

Singh

Sitholé

Lekha

et al. 2021

Bioresour. Bioprocess.

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The accumulation of petrochemical plastic waste is detrimental to the environment. Polyhydroxyalkanoates (PHAs) are bacterial-derived polymers utilized for the production of bioplastics. PHA-plastics exhibit mechanical and thermal properties similar to conventional plastics. However, high production cost and obtaining high PHA yield and productivity impedes the widespread use of bioplastics. This study demonstrates the concept of cyclic fed-batch fermentation (CFBF) for enhanced PHA productivity by Bacillus thuringiensis using a glucose-rich hydrolyzate as the sole carbon source. The statistically optimized fermentation conditions used to obtain high cell density biomass (OD600 of 2.4175) were: 8.77 g L−1 yeast extract; 66.63% hydrolyzate (v/v); a fermentation pH of 7.18; and an incubation time of 27.22 h. The CFBF comprised three cycles of 29 h, 52 h, and 65 h, respectively. After the third cyclic event, cell biomass of 20.99 g L−1, PHA concentration of 14.28 g L−1, PHA yield of 68.03%, and PHA productivity of 0.219 g L−1 h−1 was achieved. This cyclic strategy yielded an almost threefold increase in biomass concentration and a fourfold increase in PHA concentration compared with batch fermentation. FTIR spectra of the extracted PHAs display prominent peaks at the wavelengths unique to PHAs. A copolymer was elucidated after the first cyclic event, whereas, after cycles CFBF 2–4, a terpolymer was noted. The PHAs obtained after CFBF cycle 3 have a slightly higher thermal stability compared with commercial PHB. The cyclic events decreased the melting temperature and degree of crystallinity of the PHAs. The approach used in this study demonstrates the possibility of coupling fermentation strategies with hydrolyzate derived from lignocellulosic waste as an alternative feedstock to obtain high cell density biomass and enhanced PHA productivity.

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Section: Polymer Compositioncontrasting

confidence: 99%

Optimization of cultivation medium and cyclic fed-batch fermentation strategy for enhanced polyhydroxyalkanoate production by Bacillus thuringiensis using a glucose-rich hydrolyzate

Singh

Sitholé

Lekha

et al. 2021

Bioresour. Bioprocess.

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“…Despite several efforts to reduce the major barriers to production by using renewable low-cost substrates and continuously developing and assessing the biopolymer's sustainable production, only a few companies are pushing forward to produce PHA on a commercial scale in the longer term (Table 8). PHA production was projected to be 54 kilotonnes in 2014, and it is predicted to face a five-fold increment by 2020 [113]. Due to the demand from the market and healthcare industries for renewable resources, as well as recent improvements in PHA manufacturing technologies in 2017, the PHA market is expected to expand at a compound annual growth rate of about 6.3% per 10 years, with almost USD 119.15 million by 2025 (Table 9).…”

Section: Pha In Commercial Scalementioning

confidence: 99%

“…PHA production was projected to be 54 kilotonnes in 2014, and it is predicted to face a five-fold increment by 2020 [ 113 ]. Due to the demand from the market and healthcare industries for renewable resources, as well as recent improvements in PHA manufacturing technologies in 2017, the PHA market is expected to expand at a compound annual growth rate of about 6.3% per 10 years, with almost USD 119.15 million by 2025 ( Table 9 ).…”

Section: Pha In Commercial Scalementioning

confidence: 99%

Recent Advances in the Biosynthesis of Polyhydroxyalkanoates from Lignocellulosic Feedstocks

Vigneswari

Noor

Amelia

et al. 2021

Life

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Polyhydroxyalkanoates (PHA) are biodegradable polymers that are considered able to replace synthetic plastic because their biochemical characteristics are in some cases the same as other biodegradable polymers. However, due to the disadvantages of costly and non-renewable carbon sources, the production of PHA has been lower in the industrial sector against conventional plastics. At the same time, first-generation sugar-based cultivated feedstocks as substrates for PHA production threatens food security and considerably require other resources such as land and energy. Therefore, attempts have been made in pursuit of suitable sustainable and affordable sources of carbon to reduce production costs. Thus, in this review, we highlight utilising waste lignocellulosic feedstocks (LF) as a renewable and inexpensive carbon source to produce PHA. These waste feedstocks, second-generation plant lignocellulosic biomass, such as maize stoves, dedicated energy crops, rice straws, wood chips, are commonly available renewable biomass sources with a steady supply of about 150 billion tonnes per year of global yield. The generation of PHA from lignocellulose is still in its infancy, hence more screening of lignocellulosic materials and improvements in downstream processing and substrate pre-treatment are needed in the future to further advance the biopolymer sector.

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“…S5 ) according to Annamalai et al . 53 . Molasses was diluted five-fold with distilled water and centrifuged at 6,000 rpm for 20 min to separate solid materials before the acid H 2 SO 4 -heat treatment.…”

Section: Methodsmentioning

confidence: 99%

Novel research on nanocellulose production by a marine Bacillus velezensis strain SMR: a comparative study

Abouelkheir

Kamara

Atia

et al. 2020

Sci Rep

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Bacterial nanocellulose (BNC) is a nanofibrillar polymer that possesses unique characteristics such as high chemical purity, mechanical strength, flexibility, and absorbency. In addition, different bacterial strains can form nanocellulose (NC) in multiple shapes and sizes. This study describes the first report of a marine Bacillus strain that is able to synthesize NC. The strain identified as B. velezensis SMR based on 16S rDNA sequencing, produced highly structured NC, as confirmed by X-ray diffraction (XRD) and Scanning Electron Microscopic Analysis (SEM). In Hestrin-Schramm (HS) medium, B. velezensis SMR produced twice the quantity of BNC in comparison to the reference strain, G. xylinus ATCC 10245. The ability of B. velezensis SMR to produce NC using different industrial waste materials as growth media was tested. Growth in Ulva seaweed extract supported a 2.5-fold increase of NC production by B. velezensis SMR and a threefold increase in nc production by G. xylinus ATCC 10245. As proof of principle for the usability of nc from B. velezensis SMR, we successfully fabricated a BNCbased polyvinyl alcohol hydrogel (BNC-PVA) system, a promising material used in different fields of application such as medicine, food, and agriculture. Bacterial nanocellulose (BNC), an extracellular produced structure, is considered a highly desirable biomaterial due to its superior qualities in comparison to other cellulose-containing structures. Compared to plant cellulose, nanofibril network of biocellulose possesses high water retaining capacity, degree of polymerization, chemical purity, high crystallinity, in vivo biocompatibility to be used as a scaffold in tissue engineering, and excellent mechanical properties 1,2. BNC production has been reported in both Gram-negative bacteria such as G. xylinus, Agrobacterium, Achromobacter, Aerobacter, Azotobacter, Pseudomonas, and Rhizobium, and Gram-positive bacteria such as Sarcina 3. Among the different BNC-producing bacteria, G. xylinus is the most commonly studied species 4. Synthesis of BNC is a complex process involving polymerization of glucose monomers and secretion of the complex cellulose structures to the external environment to create a three-dimensional microfibril and nanofibril network. During the fermentation process, bacteria either move freely in the media or attach to cellulose fibers, producing a highly swollen gel structure 5. Purification of NC from the culture medium involves the removal of bacterial cells and collection of the cellulose matrix from the cultural medium. This is a crucial step to ensure the quality of BNC and can be performed either by repeated washing using a hot sodium hydroxide solution, followed by water until reaching a neutral pH or by other methods, such as gamma radiation 6. While BNC-production by several bacterial species have been reported, it has never been shown that members of the genus Bacillus were able to produce NC. B. velezensis is a Gram-positive bacterium that has been extensively studied for its ability to induce plant growth-...

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Biorefinery production of poly-3-hydroxybutyrate using waste office paper hydrolysate as feedstock for microbial fermentation

Cited by 55 publications

References 32 publications

Optimization of cultivation medium and cyclic fed-batch fermentation strategy for enhanced polyhydroxyalkanoate production by Bacillus thuringiensis using a glucose-rich hydrolyzate

Optimization of cultivation medium and cyclic fed-batch fermentation strategy for enhanced polyhydroxyalkanoate production by Bacillus thuringiensis using a glucose-rich hydrolyzate

Recent Advances in the Biosynthesis of Polyhydroxyalkanoates from Lignocellulosic Feedstocks

Novel research on nanocellulose production by a marine Bacillus velezensis strain SMR: a comparative study

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