Biorefinery Platform for Spathaspora passalidarum NRRL Y-27907 in the Production of Ethanol, Xylitol, and Single Cell Protein from Sugarcane Bagasse

Bonan, C.I.D.G.; Tramontina, Robson; Santos, Monick W. dos; Biazi, Luiz Eduardo; Soares, Lauren B.; Pereira, Isabela O.; Hoffmam, Zaira B.; Coutouné, Natalia; Squina, Fábio M.; Robl, Diogo; Ienczak, Jaciane Lutz

doi:10.1007/s12155-021-10255-7

Cited by 25 publications

(14 citation statements)

References 44 publications

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“…This behavior has already been reported by Biazi et al and Hou when a mixture of glucose and xylose was used for fermentation. According to Bonan et al, glucose and xylose consumption by this yeast has different ATP biosynthesis processes, and xylose presents a significantly lower cell yield than glucose because ATP demand was probably a dominant effector in these catabolic conditions. , ATP is generated on respiration and is consumed by the xylose symporter transporter and by the enzyme xylokinase for the conversion of D-xylulose to D-xylulose-5-phosphate. The low availability of ATP may, in addition to slowing fermentations due to transporter limitation, interrupt the xylose metabolism pathway and accumulate these metabolites in the medium. , For these reasons, cell growth was associated exclusively with glucose uptake.…”

Section: Resultsmentioning

confidence: 99%

“…This behavior has already been reported by Biazi et al 19 and Hou 22 when a mixture of glucose and xylose was used for fermentation. According to Bonan et al, 50 glucose and xylose consumption by this yeast has different ATP biosynthesis processes, and xylose presents a significantly lower cell yield than glucose because ATP demand was probably a dominant effector in these catabolic conditions. 50,51 ATP is generated on respiration and is consumed by the xylose symporter transporter and by the enzyme xylokinase for the conversion of D-xylulose to Dxylulose-5-phosphate.…”

Section: Methodsmentioning

confidence: 99%

“…The low availability of ATP may, in addition to slowing fermentations due to transporter limitation, interrupt the xylose metabolism pathway and accumulate these metabolites in the medium. 50,51 For these reasons, cell growth was associated exclusively with glucose uptake. In this case, a model based on Monod (eq 10) was used.…”

Section: Methodsmentioning

confidence: 99%

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Dynamic Modeling Application To Evaluate the Performance of Spathaspora passalidarum in Second-Generation Ethanol Production: Parametric Dynamics and the Likelihood Confidence Region

Martinez-Jimenez

Ribeiro

Sargo

et al. 2021

Ind. Eng. Chem. Res.

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Lignocellulosic materials and agro-industrial residues are essential in a sustainable biorefinery. The effective fermentation of renewable sugars is still an important challenge for an economically viable production of biofuels and biochemicals. This challenge involves biological aspects, such as choosing the microbial platform, and industrial aspects. These aspects can be assessed employing mathematical models to understand the complex synergies between the microorganism and processes. In this work, the performance of the yeast Spathaspora passalidarum was evaluated for the fermentation of xylose and glucose at different proportions, initial concentrations, and initial cell densities using an unstructured−unsegregated kinetic model. The likelihood confidence region, correlations between the parameters, and the convergence of the parameters through the optimization process were identified. It was also possible to identify that π Xyl, max , μ Glu, max , K ' S, Xyl , Y ' PEth/SGlu , Y ' PEth/SXyl , and Y ' X/SGlu were the most representative parameters, due to the high correlation (positive and negative) they have with the other model parameters. It was also possible to identify how initial substrate concentrations affected the specific uptake substrate and ethanol production rates; in this sense, the increase in the glucose concentration causes a decrease in the specific ethanol formation rate at a high concentration of xylose for S. passalidarum cultivated in the conditions proposed by this study.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Dynamic Modeling Application To Evaluate the Performance of Spathaspora passalidarum in Second-Generation Ethanol Production: Parametric Dynamics and the Likelihood Confidence Region

Martinez-Jimenez

Ribeiro

Sargo

et al. 2021

Ind. Eng. Chem. Res.

Self Cite

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“…Based on a standard Abs versus dry cell weight relation previously stablished by our team, it was possible to estimate dry cell weight content. The concentrations of xylose, glucose, ethanol, acetic acid, xylitol and glycerol were determined by high-performance liquid chromatography (HPLC) as described by Bonan et al [32].…”

Section: Determination Of Free Cell and Analytes Concentrationmentioning

confidence: 99%

Immobilization of Spathaspora passalidarum NRRL Y-27907 in Calcium Alginate Aiming the Production of Second-Generation Ethanol

et al. 2021

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The immobilization of S. passalidarum in calcium alginate beads for second-generation ethanol production (E2G) was evaluated in a medium that simulated a hemicellulosic hydrolysate of sugarcane bagasse pretreated with diluted sulfuric acid in terms of sugars composition. Three sets of sequential batch fermentations (SBF) were carried out with free cells, or immobilized cells in high (HSC) and moderate (MSC) initial sugar concentration (120 and 70 g/L, respectively). SBF were characterized by five consecutive batches performed in shaker, at 30 °C and 110 rpm. Better results were observed for the SBF with immobilized cells in MSC medium when compared to HSC (Y'P/S of 0.27 ± 0.02 and 0.19 ± 0.03 g/g, respectively), in the second batch cycle. The value for YP/S in MSC was similar to the obtained with free cells (0.30 ± 0.02 to 0.33 ± 0.02 g/g). However, QP was lower for MSC, reaching 0.81 ± 0.04 g/L.h in in the second batch, while for free cells the QP varied from 1.06 ± 0.02 to 1.16 ± 0.22 g/L.h. A technique for determining the concentration of immobilized cells in the alginate beads was applied, which made it possible to determine the specific rates for the SBF performed. According to the results obtained, it was possible to demonstrate that S. passalidarum can be immobilized in calcium alginate and reuse through SBF, with performance similar to free cells, which can be a good strategy for fermentation of hemicellulosic hydrolysates.

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“…Yeast biomass is also composed of lipids (0.5-8 %), carbohydrates (18-43%), minerals, and vitamins. Yeast biomass is rich in B vitamins, calcium, phosphorus, potassium, magnesium, copper, iron, zinc, manganese, and selenium [14], [23], [25], [29]. SCP is rich in certain essential amino acids, such as lysine and methionine, which are limited in most plant and animal sources.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Yeast Cultivation Factors for Improved SCP Production

Raita

Kusnere

Spalviņš

et al. 2022

Environmental and Climate Technologies

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Yeast Single-Cell Proteins (SCP) production using various agro-industrial byproducts and wastes have significant potential as an alternative to the soy meal, and fish meal protein used for livestock and aquaculture feeds. The use of organic wastes as a substrate in the fermentation processes can be accepted as one of the solutions to reduce the total price of the culture and an environmentally friendlier method of removing these residues. This review article focuses on the yeast biomass yield and protein content increase strategies, which is impossible without understanding metabolic pathways and switching mechanisms. The present work discusses optimization strategies for protein-enriched yeast biomass production, such as fermentation medium composition, including a selection of carbon and nitrogen sources and their ratio, supplemented trace elements, and cultivation conditions such as pH, temperature, time of cultivation, and inoculum size. This review summarizes the theoretical knowledge and experimental results of other researchers that provide an overview of the achievements of the last decades in the production of SCP.

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Biorefinery Platform for Spathaspora passalidarum NRRL Y-27907 in the Production of Ethanol, Xylitol, and Single Cell Protein from Sugarcane Bagasse

Cited by 25 publications

References 44 publications

Dynamic Modeling Application To Evaluate the Performance of Spathaspora passalidarum in Second-Generation Ethanol Production: Parametric Dynamics and the Likelihood Confidence Region

Dynamic Modeling Application To Evaluate the Performance of Spathaspora passalidarum in Second-Generation Ethanol Production: Parametric Dynamics and the Likelihood Confidence Region

Immobilization of Spathaspora passalidarum NRRL Y-27907 in Calcium Alginate Aiming the Production of Second-Generation Ethanol

Optimization of Yeast Cultivation Factors for Improved SCP Production

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