Bioreducible Hyperbranched Poly(amido amine)s for Gene Delivery

Chen, Jun; Wu, Chao; Oupický, David

doi:10.1021/bm900724c

Cited by 109 publications

(113 citation statements)

References 56 publications

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“…Therefore Oupicky et al prepared a complete series of reducible hyperbranched PAMAMs (RHB), which were synthesized by Michael addition copolymerization of N,Ndimethylaminodipropylene-triamine (DMDPTA) with either N,N 0 -hexamethylene bisacrylamide (HMBA) or N,N 0 -cystamine bisacrylamide (CBA) (Fig. 10) [118]. A library of compounds with different co-monomer ratios was built, with special emphasis given to the preparation of RHB with different disulfide loadings.…”

Section: Hyperbranched Pamammentioning

confidence: 99%

Hyperbranched Polyamines for Transfection

Fischer

Calderón

Haag

2010

Topics in Current Chemistry

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The successful application of gene therapy through DNA transfection into the cell is still a great challenge in ongoing research. Hyperbranched polyamines are highly branched macromolecules, and have gained significant attention in the last two decades, due to their relative ease of preparation, their shape, and their multi-functionality.This review deals with the syntheses of various hyperbranched polyamines that are prepared through a one-step polymerization process. Furthermore, we present the current status of polyamines as gene carriers and describe their versatility, and their properties such as structure-property dependency, gene transfection efficiency, and cytotoxicity profiles of hyperbranched polyamines.

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Section: Hyperbranched Pamammentioning

confidence: 99%

Hyperbranched Polyamines for Transfection

Fischer

Calderón

Haag

2010

Topics in Current Chemistry

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“…Furthermore, bioreducibility of transfection agents is essential for the release of the complexed nucleotides inside the cells with subsequent elimination of the degradation products [10]. Amongst several transfection agents explored in clinical studies so far [11,12], the supramolecular cationic cyclodextrin polymers have also yielded appreciable results [13].…”

Section: Introductionmentioning

confidence: 99%

Cellular delivery of polynucleotides by cationic cyclodextrin polyrotaxanes

Dandekar

Jain

Keil

et al. 2012

Journal of Controlled Release

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Cationic polyrotaxanes, obtained by temperature activated threading of cationic cyclodextrin derivatives onto water soluble cationic polymers (ionenes), form metastable nanometric polyplexes with pDNA and combinations of siRNA with pDNA.Because of their low toxicity, the polyrotaxane polyplexes constitute a very interesting system for the transfection of polynucleotides into mammalian cells. The complexation of Cy3-labeled siRNA within the polyplexes was demonstrated by fluorescence correlation spectroscopy. The uptake of the polyplexes (red) was imaged by confocal fluorescence microscopy using the A549 cell line as a model (blue: nuclei, green: membranes). The results prove the potential of polyrotaxanes for further investigations involving knocking down genes of therapeutic interest.

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“…Vectors such as cationic lipids and polymers have been widely studied [21][22][23]. Among those, cationic polymers, such as poly(L-lysine) (PLL) and polyethylenimine (PEI), are the best known [24].…”

Section: Introductionmentioning

confidence: 99%

Transfection of Immortalized Keratinocytes by Low Toxic Poly(2-(Dimethylamino)Ethyl Methacrylate)-Based Polymers

Overstraeten-Schlögel

Ho-Shim

Tevel

et al. 2012

Journal of Biomaterials Science, Polymer Edition

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Skin carcinoma are among the most spread diagnosed tumours in the world. In this study, we investigated the transfection of immortalized keratinocytes, used as an in vitro model for skin carcinoma, using antisense technology and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA)-based polymers, with original architecture and functionalities. We tested PDMAEMA polymers with different structures: linear, with two (DEA-PDMAEMA) or three (TEA-PDMAEMA) arms. The cytotoxicity of these polymers was assessed over a wide range of apparent M n (from 7600 to 64 600). At a N/P ratio of 7.38, cytotoxicity increases with the M n . Keratinocytes were transfected with a fluorescent oligonucleotide and then analyzed by flow cytometry. For the three architectures tested, the percentage of transfected cells and abundance of internalized oligonucleotide were closely related to the M n of the polymer. Confocal microscopy and FACS analyses showed a wide spread fine granular distribution of the oligonucleotide up to 3 days post-transfection. Then, we assessed the silencing efficiency of the polymers, targeting GFP in GFP expressing keratinocytes. The maximal silencing effect (±40%) was obtained using a DEA-PDMAEMA polymer (M n = 30 300). These results suggest that PDMAEMA-based polymers can be efficiently used to transfect immortalized keratinocytes and, thus, open new perspectives in the therapy of skin carcinoma.

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Bioreducible Hyperbranched Poly(amido amine)s for Gene Delivery

Cited by 109 publications

References 56 publications

Hyperbranched Polyamines for Transfection

Hyperbranched Polyamines for Transfection

Cellular delivery of polynucleotides by cationic cyclodextrin polyrotaxanes

Transfection of Immortalized Keratinocytes by Low Toxic Poly(2-(Dimethylamino)Ethyl Methacrylate)-Based Polymers

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