Biorecognition Layer Based On Biotin-Containing [1]Benzothieno[3,2-b][1]benzothiophene Derivative for Biosensing by Electrolyte-Gated Organic Field-Effect Transistors

Abstract

Requirements of speed and simplicity in testing stimulate the development of modern biosensors. Electrolyte-gated organic field-effect transistors (EGOFETs) are a promising platform for ultrasensitive, fast, and reliable detection of biological molecules for low-cost, point-of-care bioelectronic sensing. Biosensitivity of the EGOFET devices can be achieved by modification with receptors of one of the electronic active interfaces of the transistor gate or organic semiconductor surface. Functionalization of the latter gives the advantage in the creation of a planar architecture and compact devices for lab-on-chip design. Herein, we propose a universal, fast, and simple technique based on doctor blading and Langmuir–Schaefer methods for functionalization of the semiconducting surface of C8-BTBT-C8, allowing the fabrication of a large-scale biorecognition layer based on the novel functional derivative of BTBT-containing biotin fragments as a foundation for further biomodification. The fabricated devices are very efficient and operate stably in phosphate-buffered saline solution with high reproducibility of electrical properties in the EGOFET regime. The development of biorecognition properties of the proposed biolayer is based on the streptavidin–biotin interactions between the consecutive layers and can be used for a wide variety of receptors. As a proof-of-concept, we demonstrate the specific response of the BTBT-based biorecognition layer in EGOFETs to influenza A virus (H7N1 strain). The elaborated approach to biorecognition layer formation is appropriate but not limited to aptamer-based receptor molecules and can be further applied for fabricating several biosensors for various analytes on one substrate and paves the way for “electronic tongue” creation.

Magnetic carbon gate electrodes for the development of electrolyte-gated organic field effect transistor bio-sensing platforms

“…Similarly, the present EGOFET is expected to be employed for the detection of antigen-antibody, nucleic acid systems and other bioactive macromolecules. The potential of the present work is the combination of the ultra-sensitive detection of EGOFETs 5,10,14,15,25,53 with the fabrication of cheap and reusable CGE for developing point-of-care sensors.…”

Healthcare Monitoring Sensors Based on Organic Transistors: Surface/Interface Strategy and Performance

“…[ 214 ] Copyright 2017, Elsevier B.V. f) A EGOFET with BTBT‐C8 as channel layer and a BTBT‐C8 functional derivatives as receptor layer targeting for influenza A virus detection; Reproduced with permission. [ 218 ] Copyright 2022, American Chemical Society.…”

“…e) Physical absorption of antibody on the P3HT channel layer and their sensitive response to PCT; Reproduced with permission [214]. Copyright 2017, Elsevier B.V. f) A EGOFET with BTBT-C8 as channel layer and a BTBT-C8 functional derivatives as receptor layer targeting for influenza A virus detection; Reproduced with permission [218]. Copyright 2022, American Chemical Society.…”

New Opportunities for Organic Semiconducting Polymers in Biomedical Applications

“…The dielectric contact-free intrinsic mobility reached 40 cm 2 ·V −1 ·s −1 [ 23 ]. In one biosensing application, an electrolyte gate FET was fabricated using C 8 -BTBT/polystyrene as a semiconductor and a blend of BTBT-biotin and a siloxane dimer of BTBT as an influenza virus-detecting layer, which was modified with streptavidin and the DNA aptamer RHA0385 [ 24 ].…”