Bioreactors for Vocal Fold Tissue Engineering

Martins, Ana Maria Gracioso; Biehl, Andreea; Sze, Daphne; Freytes, Donald O.

doi:10.1089/ten.teb.2020.0285

Cited by 2 publications

(4 citation statements)

References 176 publications

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“…To further exploit the sophistication of this platform, various cell types specific to each layer of the vocal tissue could be integrated to explore their response to the vibratory regime. Namely, epithelial cells which can be found in the epithelium of the vocal fold and fibroblasts which are found in the lamina propria of the vocal fold [26]. Understanding the impact that phonation and the associated mechanical stimuli have on the vocal fold microenvironment in different anatomical positions would allow us to better study therapeutic efficacy on injured VF tissue in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…Understanding the impact that phonation and the associated mechanical stimuli have on the vocal fold microenvironment in different anatomical positions would allow us to better study therapeutic efficacy on injured VF tissue in vitro. Current bioreactors, as shown in the review by Martins et al, were able to incorporate a single cell type at a time [26]. The overall goal is to incorporate multiple cell types simultaneously to mimic the native composition of the VFs more closely.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, advances in biomaterials and cell therapy research have led to novel treatments for voice disorders such as mesenchymal stem cell (MSC) transplants and injection augmentation using bulking agents (e.g., collagen, hyaluronic acid, carboxymethylcellulose, calcium hydroxyapatite, micronized dermis, autologous fat, etc.). Although these treatments have shown promise, none has fully satisfied the regeneration of injured VFs [26]. Recent attempts at bioengineered vocal fold mucosa show promise but are not yet ready for human implantation [27].…”

Section: Introductionmentioning

confidence: 99%

“…the total number of experimental units that a bioreactor setting can allow should be considered. In the review by Martins et al [26], the authors have identified that existing bioreactors have a relatively low number of experimental units; the highest number was identified for the system developed by Bartlett et al [29] with 16 units.…”

Section: Introductionmentioning

confidence: 99%

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Scalable and High-Throughput In Vitro Vibratory Platform for Vocal Fold Tissue Engineering Applications

Biehl,

Colmon,

Timofeeva

et al. 2023

Bioengineering

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The vocal folds (VFs) are constantly exposed to mechanical stimulation leading to changes in biomechanical properties, structure, and composition. The development of long-term strategies for VF treatment depends on the characterization of related cells, biomaterials, or engineered tissues in a controlled mechanical environment. Our aim was to design, develop, and characterize a scalable and high-throughput platform that mimics the mechanical microenvironment of the VFs in vitro. The platform consists of a 24-well plate fitted with a flexible membrane atop a waveguide equipped with piezoelectric speakers which allows for cells to be exposed to various phonatory stimuli. The displacements of the flexible membrane were characterized via Laser Doppler Vibrometry (LDV). Human VF fibroblasts and mesenchymal stem cells were seeded, exposed to various vibratory regimes, and the expression of pro-fibrotic and pro-inflammatory genes was analyzed. Compared to current bioreactor designs, the platform developed in this study can incorporate commercial assay formats ranging from 6- to 96-well plates which represents a significant improvement in scalability. This platform is modular and allows for tunable frequency regimes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Scalable and High-Throughput In Vitro Vibratory Platform for Vocal Fold Tissue Engineering Applications

Biehl,

Colmon,

Timofeeva

et al. 2023

Bioengineering

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Novel application of bone marrow mesenchymal stem cells combined with hepatocyte growth factor on subacute vocal fold wound healing

Xu,

Meng,

Wang

et al. 2024

World j. otorhinolaryngol.-head neck surg.

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ObjectivesVocal fold (VF) scar causes solemn vocal problems for patients with previous surgery or laryngeal injury. It is also a difficult management problem for clinicians since rare superior management methods are available at present. Previous research usually focuses on the acute period of VF wound repairing, with little attention on subacute period intervention. Bone marrow mesenchymal stem cells (BMMSCs) and hepatocyte growth factor (HGF) combinations that are applied to treat VF wounds are rarely reported. The current study investigated the effects of BMMSCs‐HGF application on the regeneration of the VF mucosa during the subacute period.MethodsA bilateral wound was generated by forceps in the VF of each New Zealand White rabbit. BMMSCs are based on the acellular dermal matrix as cell carriers. Various compounds (BMMSCs‐AMD complex with or without HGF) was injected into the wounded VF at 2‐ or 4‐weeks following injury. Animals were killed at 60 days postinjection. Imaging of excised larynges was performed with the transmission electron microscope (TEM), and Immunohistochemical (IHC) analysis was also conducted.ResultsCollectively, 18 rabbits accepted injury modeling, with two of them died before repairing intervention. IHC results indicated that type‐I collagen was significantly devalued in the cluster injected with BMMSCs‐HGF than simple application of BMMSCs. The results of TEM suggested that the BMMSCs‐HGF injection can significantly reduce the collagen score.ConclusionOur preliminary study suggests opening regenerative effects of intervention for VF subacute scaring, with BMMSCs‐HGF.

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Bioreactors for Vocal Fold Tissue Engineering

Cited by 2 publications

References 176 publications

Scalable and High-Throughput In Vitro Vibratory Platform for Vocal Fold Tissue Engineering Applications

Scalable and High-Throughput In Vitro Vibratory Platform for Vocal Fold Tissue Engineering Applications

Novel application of bone marrow mesenchymal stem cells combined with hepatocyte growth factor on subacute vocal fold wound healing

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