Bioreactors and Image Analysis for Scale-Up and Plant Propagation

Cazzulino, Dana; Pedersen, Henrik; Chin, Chee‐Kok

doi:10.1016/b978-0-12-715008-6.50015-4

Cited by 28 publications

(15 citation statements)

References 32 publications

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“…Progress in building the technology necessary for a truly continuous culture system has been made in the form of a kinetic model of carrot somatic embryo development in suspension culture, in which substrate utilization, culture growth, and embryo development are monitored over the time course of an embryogenic culture [144]. The integration of such a model with image analysis hardware and software for monitoring embryo concentration and development will be a key to establishing an automated system for continuous somatic embryo production in bioreactors [145].…”

Section: Bioreactorsmentioning

confidence: 99%

Morphogenic Aspects of Somatic Embryogenesis

Merkle¹,

Parrott²,

Flinn³

1995

In Vitro Embryogenesis in Plants

210

146

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Section: Bioreactorsmentioning

confidence: 99%

Morphogenic Aspects of Somatic Embryogenesis

Merkle¹,

Parrott²,

Flinn³

1995

In Vitro Embryogenesis in Plants

210

146

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“…The software was first established by Cazzulino et al (1990Cazzulino et al ( , 1991 and modified to enable automatic counting of each embryo stage through a Model 3000 Image Analyzer (Image Technology, Deer Park, N.Y.). The software was first established by Cazzulino et al (1990Cazzulino et al ( , 1991 and modified to enable automatic counting of each embryo stage through a Model 3000 Image Analyzer (Image Technology, Deer Park, N.Y.).…”

Section: Methodsmentioning

confidence: 99%

Restoration of embryogenic competence in repeatedly subcultured carrot cell lines

1992

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The production of somatic embryos from carrot suspension cultures invariably decreases through simple, repeated subcutturing. Extracellular, concentrated compounds extracted from already established embryo culture not only recovered the embryogenic capability, but also accelerated the embryo production as much as two-fold (up to 1600 embryos/ml) compared with that of a control culture. Sugars, which were only a small portion of the total concentrate, were excluded as possibl e causative factors. It is likely that a protein fraction that is generated directly by competent, embryogenic cultures is important for the restoration of embryogenic potential.

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“…The situation is even more complex when Fourier descriptors are used as it is difficult to find a real physical significance to them. Data-mining tools such as Principal Component Analysis [90,135,157,158], Discriminant Analysis [82], fuzzy logic and Artificial Neural Nets [41,72,95,132,133,159] are useful for clarifying the situation.…”

Section: ) Pellet Convex Area -Core Convex Areamentioning

confidence: 99%

Biomass Quantification by Image Analysis

Pons

Vivier

1999

Bioanalysis and Biosensors for Bioprocess Monitoring

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Microbiologists have always rely on microscopy to examine microorganisms. When microscopy, either optical or electron-based, is coupled to quantitative image analysis, the spectrum of potential applications is widened: counting, sizing, shape characterization, physiology assessment, analysis of visual texture, motility studies are now easily available for obtaining information on biomass. In this chapter the main tools used for cell visualization as well as the basic steps of image treatment are presented. General shape descriptors can be used to characterize the cell morphology, but special descriptors have been defined for filamentous microorganisms. Physiology assessment is often based on the use of fluorescent dyes. The quantitative analysis of visual texture is still limited in bioengineering but the characterization of the surface of microbial colonies may open new prospects, especially for cultures on solid substrates. In many occasions, the number of parameters extracted from images is so large that data-mining tools, such as Principal Components Analysis, are useful for summarizing the key pieces of information.

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Bioreactors and Image Analysis for Scale-Up and Plant Propagation

Cited by 28 publications

References 32 publications

Morphogenic Aspects of Somatic Embryogenesis

Morphogenic Aspects of Somatic Embryogenesis

Restoration of embryogenic competence in repeatedly subcultured carrot cell lines

Biomass Quantification by Image Analysis

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