Bioreactor shoot cultures of Rhododendron tomentosum (Ledum palustre) for a large-scale production of bioactive volatile compounds

Jesionek, Anna; Kokotkiewicz, Adam; Wlodarska, Paulina; Zabiegała, Bożena; Buciński, Adam; Łuczkiewicz, Maria

doi:10.1007/s11240-017-1261-0

Cited by 32 publications

(15 citation statements)

References 47 publications

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“…The model of agitated shoot cultures or liquid whole plantlet cultures, which aimed at optimizing the biosynthetic capabilities of cultured species and accumulating the desired metabolites was employed earlier by other authors, for example, in cultures of Centella asiatica (Kim et al 2004), Aronia arbutifolia, A. × prunifolia (Szopa et al 2018), Hypericum perforatum (Kwiecień et al 2015), Knautia sarajevensis (Karalija et al 2017), and Rhododendron tomentosum (Jesionek et al 2017).…”

Section: Discussionmentioning

confidence: 99%

“…The advantages of this model include more efficient saturation of medium with gasses and continuous agitation of the medium leading to the equal distribution of nutrients and their better availability, associated with increased production of secondary metabolites (Jesionek et al 2017). Additionally, in the case of L. flos-cuculi, it positively affects the rate of rhizogenesis.…”

Section: Discussionmentioning

confidence: 99%

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Various in vitro systems of Ragged Robin (Lychnis flos-cuculi L.): a new potential source of phytoecdysteroids?

Maliński

Kikowska

Kruszka

et al. 2019

Plant Cell Tiss Organ Cult

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Lychnis flos-cuculi L. is a species containing ecdysteroids, triterpenoid saponins, flavonoids, and phenolic acids, and therefore is a plant of potential medicinal value. In the presented research, diverse in vitro cultures of this taxon were developed to obtain the uniform material capable of producing ecdysteroids, including micropropagated plantlets, shoot cultures, liquid agitated whole plant cultures with fast-growing roots, and callus. A protocol of micropropagation through axillary bud formation was established using plant growth regulators at different concentrations and combinations. All the variants of plant growth regulator supplementation significantly affected a shoot proliferation rate ranging from 8 to 16 plantlets per explant, depending on the medium; DNA content of all the studied plant materials was similar. The thin-layer chromatography analysis of the extracts revealed the presence of ecdysteroids in every plant material apart from callus. The content of 20-hydroxyecdysone and polypodine B was evaluated by high-performance liquid chromatography. Agitated plantlets were in vitro cultures that efficiently formed abundant root biomass with significant concentrations of ecdysteroids. In vitro-derived adventitious roots contained twofold higher content of ecdysteroids than those of intact plants. The organs of flowering in vitro-propagated plants, transferred to experimental plot, contained twice as much ecdysteroids when compared to the organs of plants from the natural site, among which flowers were the richest in ecdysteroids. The results revealed that adventitious roots from L. flos-cuculi agitated cultures can be considered as an alternative biotechnological source of biomass rich in pharmaceutically active ecdysteroids. Key message Diverse types of Lychnis flos-cuculi in vitro cultures have been developed. HPLC analysis demonstrated much higher ecdysteroid content in cultures and regenerated plants when compared to natural site plants. Keywords Micropropagation • Callus • 20-Hydroxyecdysone • Polypodine B • Agitated plant cultures • Genome size Abbreviations 2,4-D 2,4-Dichlorophenoxyacetic acid 20HE 20-Hydroxyecdysone BAP N 6-benzylaminopurine Dic Dicamba (3,6-dichloro-2-methoxybenzoic acid) IAA Indole-3-acetic acid Kin Kinetin (N 6-furfuryladenine) MS Murashige & Skoog medium NAA 1-Naphthaleneacetic acid PGRs Plant growth regulators Pic Picloram (4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid) polB Polypodine B (5β,20-dihydroxyecdysone) TDZ Thidiazuron (1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea TLC Thin-layer chromatography Communicated by Ali R. Alan.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Various in vitro systems of Ragged Robin (Lychnis flos-cuculi L.): a new potential source of phytoecdysteroids?

Maliński

Kikowska

Kruszka

et al. 2019

Plant Cell Tiss Organ Cult

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“…In addition, the derivatives of protocatechuic (14), caffeic (15,18,22), phenolic (derivatives) (17,21) and sinapic acids (29) were detected. Among the flavonoids, rutoside (26) was detected in these extracts. The presence of three undefined compounds (16,31,32) was also noted (Table 3, Figure 3).…”

Section: Chromatographic Analysis Of Polyphenol Contentsmentioning

confidence: 98%

Phytochemical and Biological Activity Studies on Nasturtium officinale (Watercress) Microshoot Cultures Grown in RITA® Temporary Immersion Systems

et al. 2020

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The main compounds in both extracts were gluconasturtiin, 4-methoxyglucobrassicin and rutoside, the amounts of which were, respectively, determined as 182.93, 58.86 and 23.24 mg/100 g dry weight (DW) in biomass extracts and 640.94, 23.47 and 7.20 mg/100 g DW in plant herb extracts. The antioxidant potential of all the studied extracts evaluated using CUPRAC (CUPric Reducing Antioxidant Activity), FRAP (Ferric Reducing Ability of Plasma), and DPPH (1,1-diphenyl-2-picrylhydrazyl) assays was comparable. The anti-inflammatory activity of the extracts was tested based on the inhibition of 15-lipoxygenase, cyclooxygenase-1, cyclooxygenase-2 (COX-2), and phospholipase A2. The results demonstrate significantly higher inhibition of COX-2 for in vitro cultured biomass compared with the herb extracts (75.4 and 41.1%, respectively). Moreover, all the studied extracts showed almost similar antibacterial and antifungal potential. Based on these findings, and due to the fact that the growth of in vitro microshoots is independent of environmental conditions and unaffected by environmental pollution, we propose that biomass that can be rapidly grown in RITA® bioreactors can serve as an alternative source of bioactive compounds with valuable biological properties.

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“…Advances in in vitro culture techniques such as TIS have recently been developed to improve mass propagation efficiency that was difficult to accomplish using conventional propagation systems such as SSS or CIS [17]. TIS mass propagation success has previously been reported for various plant species [18,19]-including orchids [11,20,21]. Prior reports revealed that different culture systems resulted in diverse growth and morphogenesis of cultured plants [11,14,22].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Propagation of Rheophytic Orchid, Epipactis flava Seidenf.—A Comparison of Semi-Solid, Continuous Immersion and Temporary Immersion Systems

Kunakhonnuruk

Inthima

Kongbangkerd

2019

Biology

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Epipactis flava Seidenf. is an endangered Thai rheophytic orchid that has recently shown a rapid decrease in its natural habitat, prompting an urgent need for conservation using ex situ reintroduction methods. Temporary immersion system (TIS) has been successfully applied for large-scale propagation in various plants species. Propagation efficiency of E. flava using TIS was investigated and compared with conventional semi-solid system (SSS) and liquid continuous immersion system (CIS). The highest percentage of new shoot and shoot bud formation was obtained from TIS, followed by CIS and SSS, respectively. Growth parameters as indicated by number of new shoots, shoot buds, shoot height and leaves per explant were significantly higher using TIS than with SSS and CIS. Moreover, the maximum number of new shoots and shoot buds per replication were reliably obtained from TIS higher than SSS and CIS. After acclimatization, the highest survival percentage of plantlets was observed in TIS (76.7%), with 60% surviving after eight weeks of transplantation in artificial stream. TIS was determined as the most suitable culture system for in vitro mass propagation of E. flava compared to CIS and SSS.

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Bioreactor shoot cultures of Rhododendron tomentosum (Ledum palustre) for a large-scale production of bioactive volatile compounds

Cited by 32 publications

References 47 publications

Various in vitro systems of Ragged Robin (Lychnis flos-cuculi L.): a new potential source of phytoecdysteroids?

Various in vitro systems of Ragged Robin (Lychnis flos-cuculi L.): a new potential source of phytoecdysteroids?

Phytochemical and Biological Activity Studies on Nasturtium officinale (Watercress) Microshoot Cultures Grown in RITA® Temporary Immersion Systems

In Vitro Propagation of Rheophytic Orchid, Epipactis flava Seidenf.—A Comparison of Semi-Solid, Continuous Immersion and Temporary Immersion Systems

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