Bioreactor-Grown Shoot Cultures for the Secondary Metabolite Production

Krol, Agata; Kokotkiewicz, Adam; Szopa, Agnieszka; Ekiert, Halina; Łuczkiewicz, Maria

doi:10.1007/978-3-030-11253-0_34-1

Cited by 6 publications

(5 citation statements)

References 128 publications

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“…The culture biomass can thus be used in pharmaceutical, cosmetological, and agricultural industries. Furthermore, the large-scale plant in vitro cultures allow for producing those compounds with limited natural resources or resources threatened by overexploitation [ 1 , 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…TIS enables the cultivation of plant in vitro cultures in submerged and nonsubmerged cycles. In this system, in vitro cultures are maintained by immersing in liquid medium without mechanical stress and with good gas exchange [ 1 , 2 , 3 ]. Several plant in vitro cultures have been cultured in the TIS bioreactors for optimizing the production of secondary metabolites.…”

Section: Introductionmentioning

confidence: 99%

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Phytochemical and Biological Activity Studies on Nasturtium officinale (Watercress) Microshoot Cultures Grown in RITA® Temporary Immersion Systems

et al. 2020

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The main compounds in both extracts were gluconasturtiin, 4-methoxyglucobrassicin and rutoside, the amounts of which were, respectively, determined as 182.93, 58.86 and 23.24 mg/100 g dry weight (DW) in biomass extracts and 640.94, 23.47 and 7.20 mg/100 g DW in plant herb extracts. The antioxidant potential of all the studied extracts evaluated using CUPRAC (CUPric Reducing Antioxidant Activity), FRAP (Ferric Reducing Ability of Plasma), and DPPH (1,1-diphenyl-2-picrylhydrazyl) assays was comparable. The anti-inflammatory activity of the extracts was tested based on the inhibition of 15-lipoxygenase, cyclooxygenase-1, cyclooxygenase-2 (COX-2), and phospholipase A2. The results demonstrate significantly higher inhibition of COX-2 for in vitro cultured biomass compared with the herb extracts (75.4 and 41.1%, respectively). Moreover, all the studied extracts showed almost similar antibacterial and antifungal potential. Based on these findings, and due to the fact that the growth of in vitro microshoots is independent of environmental conditions and unaffected by environmental pollution, we propose that biomass that can be rapidly grown in RITA® bioreactors can serve as an alternative source of bioactive compounds with valuable biological properties.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phytochemical and Biological Activity Studies on Nasturtium officinale (Watercress) Microshoot Cultures Grown in RITA® Temporary Immersion Systems

et al. 2020

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“…This involves in vitro cultivation of seedlings, stem cuttings, nodal segments, shoots, normal, adventitious or transformed “hairy” roots, somatic embryos, etc., widely known as differentiated plant in vitro systems. In contrast to undifferentiated plant in vitro systems, differentiated cultures are characterized by better genetic and biosynthetic stabilities and ability to produce and accumulate higher concentrations of certain specific metabolites whose biosynthesis occurs in specific plant tissues [6, 29‐31]. On the other hand, mass propagation of differentiated plant in vitro cultures is a costly and laborious process which requires specialized and expensive equipment [28, 32, 33].…”

Section: Cultivation Of Plant Cell Tissue and Organ Cultures With Emmentioning

confidence: 99%

“…To be cost‐effective, commercial production of plant cells or tissue biomass requires scale‐up of the process to the necessary volumes which can ensure a continuous supply. In this aspect, various bioreactor systems modified for growing undifferentiated or differentiated plant in vitro cultures can be adopted [28, 31–33, 35]. It is important to note that if the products are intended to be used in cosmetics, cultivation could be performed in small or medium volume bioreactors, or even in a solid medium (in multiple containers) or in small volumes agitated liquid medium (in multiple shaking flasks), whereas, if foods are the target market, cultivation should be conducted in large volume vessels.…”

Section: Bioreactor Systems Used For Large‐scale Production Of Foods mentioning

confidence: 99%

“…Obviously, different types of bioreactors need to be implemented depending on the type of in vitro system and the nature of the ingredient produced. However, for optimizing the initial investment made in the equipment, disposable bioreactors as well as unconventional decisions such as temporary immersion systems should be considered [14, 28, 31, 33–35, 38, 39].…”

Section: Bioreactor Systems Used For Large‐scale Production Of Foods mentioning

confidence: 99%

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Recent applications of plant cell culture technology in cosmetics and foods

Krasteva

Georgiev

Pavlov

2020

Engineering in Life Sciences

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Plants have been used as the main source of phytochemicals with nutritional, medicinal, cultural and cosmetic applications since times immemorial. Nowadays, achieving sustainable development, global climate change, restricted access to fresh water, limited food supply and growing energy demands are among the critical global challenges faced by humanity. Plant cell culture technology has the potential to address some of these challenges by providing effective tools for sustainable supply of phyto‐ingredients with reduced energy, carbon and water footprints. The main aim of this review is to discuss the recent trends in the development of plant cell culture technologies for production of plant‐derived substances with application in food products and cosmetic formulations. The specific technological steps and requirements for the final products are discussed in the light of the advances in cultivation technologies used for growing differentiated and undifferentiated plant in vitro systems. Future prospects and existing challenges of the commercialization of plant cell culture‐derived products have been outlined through the prism of the authors’ point of view. We expect this review will encourage scientists, policymakers and business enterprises to join efforts for speeding‐up the mass commercialization and popularization of plant cell culture technology as an eco‐friendly alternative method for sustainable production of plant‐derived additives with application in food and cosmetic products.

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