Bioreactor-based advances in plant tissue and cell culture: challenges and prospects

Valdiani, Alireza; Hansen, Jon Kehlet; Nielsen, Ulrik B.; Johannsen, Vivian Kvist; Shariat, Maryam; Georgiev, Milen I.; Omidvar, Vahid; Ebrahimi, Mortaza; Dinanai, Elham Tavakoli; Abiri, Rambod

doi:10.1080/07388551.2018.1489778

Cited by 78 publications

(54 citation statements)

References 136 publications

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“…The application of a suitable bioreactor system is crucial for plant cell cultivation in respect to biomass accumulation, cell viability and product formation (Eibl and Eibl 2008;Valdiani et al 2019). While stirred-tank reactors are routinely used for plant cell culture (Arias et al 2017;Pérez-Hernández et al 2019), the mechanical stirring poses a source of hydrodynamic stress for the plant cells, decreasing cell viability during the process (Takeda et al 1994).…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Establishment and triterpenoid production of Ocimum basilicum cambial meristematic cells

Mehring

Haffelder

Chodorski

et al. 2020

Plant Cell Tiss Organ Cult

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The application of plant suspension culture to produce valuable compounds, such as the triterpenoids oleanolic acid and ursolic acid, is a well-established alternative to the cultivation of whole plants. Cambial meristematic cells (CMCs) are a growing field of research, often showing superior cultivation properties compared to their dedifferentiated cell (DDC) counterparts. In this work, the first-time establishment of O. basilicum CMCs is demonstrated. DDCs and CMCs were cultivated in shake flasks and wave-mixed disposable bioreactors (wDBRs) and evaluated regarding triterpenoid productivity and biomass accumulation. CMCs showed characteristic small vacuoles and were found to be significantly smaller than DDCs. Productivities of oleanolic and ursolic acid of CMCs were determined at 3.02 ± 0.76 mg/(l*d) and 4.79 ± 0.48 mg/(l*d) after 19 days wDBR cultivation, respectively. These values were consistently higher than any productivities determined for DDCs over the observed cultivation period of 37 days. Elicitation with methyl jasmonate of DDCs and CMCs in shake flasks resulted in increased product contents up to 48 h after elicitor addition, with the highest increase found in CMCs at 232.30 ± 19.33% (oleanolic acid) and 192.44 ± 18.23% (ursolic acid) after 48 h.

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Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Establishment and triterpenoid production of Ocimum basilicum cambial meristematic cells

Mehring

Haffelder

Chodorski

et al. 2020

Plant Cell Tiss Organ Cult

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show abstract

“…The scaling-up of plant in vitro systems cultivation into large-scale bioreactors is the final step of a bioprocess for continuous and sustainable production of low-volume and high-value bioactive molecules. Economically feasible bioreactor cultivation must guarantee high SMs productivity, yield and concentration by selecting optimal bioreactor design and operating conditions [36], thus ensuring adequate mixing (facilitate nutrient transfer, prevent accumulation of toxic metabolites and simultaneously maintain a low shear stress environment), gas exchange (deliver sufficient O 2 or CO 2 for respiration) and homogeneity (prevent cell sedimentation) [37].…”

Section: The Production Of Plant Secondary Metabolites In Bioreactorsmentioning

confidence: 99%

Green (cell) factories for advanced production of plant secondary metabolites

Marchev

Yordanova

Georgiev

2020

Critical Reviews in Biotechnology

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120

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For centuries plants have been intensively utilized as reliable sources of food, flavoring, agrochemical and pharmaceutical ingredients. However, plant natural habitats are being rapidly lost due to climate change and agriculture. Plant biotechnology offers a sustainable method for the bioproduction of plant secondary metabolites using plant in vitro systems. The unique structural features of plant-derived secondary metabolites, such as their safety profile, multi-target spectrum and "metabolite likeness," have led to the establishment of many plant-derived drugs, comprising approximately a quarter of all drugs approved by the Food and Drug Administration and/or European Medicinal Agency. However, there are still many challenges to overcome to enhance the production of these metabolites from plant in vitro systems and establish a sustainable large-scale biotechnological process. These challenges are due to the peculiarities of plant cell metabolism, the complexity of plant secondary metabolite pathways, and the correct selection of bioreactor systems and bioprocess optimization. In this review, we present an integrated overview of the possible avenues for enhancing the biosynthesis of high-value marketable molecules produced by plant in vitro systems. These include metabolic engineering and CRISPR/Cas9 technology for the regulation of plant metabolism through overexpression/repression of single or multiple structural genes or transcriptional factors. The use of NMR-based metabolomics for monitoring metabolite concentrations and additionally as a tool to study the dynamics of plant cell metabolism and nutritional management is discussed here. Different types of bioreactor systems, their modification and optimal process parameters for the labor industrial-scale production of plant secondary metabolites are specified.

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“…Brief information about the bioreactors most frequently used for the propagation of trees is given below. For detailed information on these topics, readers are referred to several comprehensive reviews [21,[24][25][26][27][32][33][34][35].…”

Section: Bioreactors Systems For the Propagation Of Treesmentioning

confidence: 99%

Use of bioreactor systems in the propagation of forest trees

Vidal

Sánchez

2019

Engineering in Life Sciences

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Plant biotechnology can be used to conserve the germplasm of natural forests, and to increase the productivity and sustainability of plantations. Both goals imply working with mature trees, which are often recalcitrant to micropropagation. Conventional in vitro culture uses closed containers and gelled medium with sugar supplementation. Bioreactor culture uses liquid medium and usually incorporates aeration. The increased absorption of nutrients via the liquid medium together with the renewal of the air inside the bioreactors may improve the physiological state of the explants. In this review, we will explore the feasibility of using bioreactors to overcome the recalcitrance of many trees to micropropagation and/or to decrease the cost of large‐scale propagation. We will focus on the recent use of bioreactors during the multiplication, rooting (plant conversion in the case of somatic embryos), and acclimation stages of the micropropagation of axillary shoots and somatic embryos of forest trees (including some shrubs of commercial interest), in both temporary and continuous immersion systems. We will discuss the advantages and the main obstacles limiting the widespread implementation of bioreactor systems in woody plant culture, considering published scientific reports and contributions from the business sector.

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Bioreactor-based advances in plant tissue and cell culture: challenges and prospects

Cited by 78 publications

References 136 publications

Establishment and triterpenoid production of Ocimum basilicum cambial meristematic cells

Establishment and triterpenoid production of Ocimum basilicum cambial meristematic cells

Green (cell) factories for advanced production of plant secondary metabolites

Use of bioreactor systems in the propagation of forest trees

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