Bioprospection of Eugenia brasiliensis, a Brazilian native fruit, as a source of anti-inflammatory and antibiofilm compounds

Lazarini, Josy Goldoni; Sardi, Janaína de Cássia Orlandi; Franchin, Marcelo; Nani, Bruno Dias; Freires, Irlan Almeida; Infante, Juliana; Paschoal, Jonas Augusto Rizzato; Alencar, Severino Matias de; Rosalen, Pedro Luiz

doi:10.1016/j.biopha.2018.03.034

Cited by 53 publications

(45 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although this extract showed in vitro antibacterial activity (MIC and MBC) against Staphylococcus aureus ATCC 25923 (MSSA), Staphylococcus aureus ATCC 33591 (MRSA), Pseudomonas aeruginosa ATCC 27853, Streptococcus mutans ATCC 700610, Escherichia coli ATCC 43895 and Lactobacillus acidophilus ATCC 4356 together with an in vitro effect on mature biofilm survival, it did not exert toxic effects on the larvae when administered at antibiofilm concentrations (10 × MIC −625 μg/mL, which corresponds to doses of 0.025 g/kg of extract). Moreover, the authors did not find the lethal doses able to kill 50% (LD 50 ) of the larvae [97].…”

Section: In Vivo Toxicity Screeningmentioning

confidence: 95%

See 1 more Smart Citation

Galleria mellonella as a consolidated in vivo model hosts: New developments in antibacterial strategies and novel drug testing

et al. 2019

View full text Add to dashboard Cite

A greater ethical conscience, new global rules and a modified perception of ethical consciousness entail a more rigorous control on utilizations of vertebrates for in vivo studies. To cope with this new scenario, numerous alternatives to rodents have been proposed. Among these, the greater wax moth Galleria mellonella had a preponderant role, especially in the microbiological field, as demonstrated by the growing number of recent scientific publications. The reasons for its success must be sought in its peculiar characteristics such as the innate immune response mechanisms and the ability to grow at a temperature of 37°C. This review aims to describe the most relevant features of G. mellonella in microbiology, highlighting the most recent and relevant research on antibacterial strategies, novel drug tests and toxicological studies. Although solutions for some limitations are required, G. mellonella has all the necessary host features to be a consolidated in vivo model host.

show abstract

Section: In Vivo Toxicity Screeningmentioning

confidence: 95%

“…with a high phenol content. The authors failed to find an LD 50 using G. mellonella as in vivo model [97].…”

Section: Limitationmentioning

confidence: 99%

Galleria mellonella as a consolidated in vivo model hosts: New developments in antibacterial strategies and novel drug testing

et al. 2019

View full text Add to dashboard Cite

show abstract

“…In another study on Australian Syzygium species, the methanol fruit extracts of S. australe had a MIC of 2.5 mg/mL against E. coli while the ethyl acetate extract of S. leuhmannii had an inhibitory concentration of 0.8 mg/mL against E. coli [66] . The hydro-alcoholic crude extract of Eugenia brasiliensis had an MIC of > 2 mg/mL against E. coli (ATCC 43895) [67].…”

Section: Discussionmentioning

confidence: 99%

Antibacterial activity and mode of action of acetone crude leaf extracts of under-investigated Syzygium and Eugenia (Myrtaceae) species on multidrug resistant porcine diarrhoeagenic Escherichia coli

et al. 2019

View full text Add to dashboard Cite

Background Diarrhoea, a global economically important disease burden affecting swine and, especially piglets, is commonly caused by infection with entero-toxigenic E. coli (ETEC). Adherence of ETEC to porcine intestinal epithelial cells following infection, is necessary for its pathogenesis. While antimicrobials are commonly given as therapy or as feed additives for prophylaxis against microbial infections, the concern over increased levels of antimicrobial resistance necessitate the search for safe and effective alternatives in livestock feed. Attention is shifting to natural products including plants as suitable alternatives to antimicrobials. The activity of acetone crude leaf extracts of nine under-explored South African endemic plants from the Myrtaceae family with good antimicrobial activity were tested against pathogenic E. coli of porcine origin using a microplate serial dilution method. Bioautography, also with p-iodonitrotetrazolium violet as growth indicator was used to view the number of bioactive compounds in each extract. In vitro toxicity of extracts was determined against Caco-2 cells using the 3-(4,5-dimethythiazolyl-2)-2,5-diphenyltetrazolium bromide reduction assay. The antimicrobial susceptibility of E. coli isolates was tested on a panel of antimicrobials using the Kirby-Bauer agar diffusion method while the anti-adherence mechanism was evaluated using a Caco-2 cell enterocyte anti-adhesion model. Results The MIC of the extracts ranged from 0.07–0.14 mg/mL with S. legatii having the best mean MIC (0.05 mg/mL). Bioautography revealed at least two active bands in each plant extract. The 50% lethal concentration (LC 50 ) values ranged between 0.03–0.66 mg/mL. Eugenia zeyheri least cytotoxic (LC 50 = 0.66 mg/ml) while E. natalitia had the highest cytotoxicity (LC 50 = 0.03 mg/mL). All the bacteria were completely resistant to doxycycline and colistin sulphate and many of the plant extracts significantly reduced adhesion of E. coli to Caco-2 cells. Conclusions The extracts of the plants had good antibacterial activity as well as a protective role on intestinal epithelial cells against enterotoxigenic E. coli bacterial adhesion. This supports the potential use of these species in limiting infection causes by E. coli . Some of these plants or extracts may be useful as phytogenic feed additives but it has to be investigated by animal feed trials.

show abstract

“…By comparing the polyphenol content and antioxidant capacity with other fruits, grumixama is a highly valuable fruit due to its polyphenol content, mainly anthocyanin, which is responsible for its purple colour (LAZARINI et al, 2018), and tannins (Table 2). Thus, in vivo studies should be conducted to investigate the beneficial properties of grumixama and its by-products on different metabolic systems, as well as on the gut microbiota.…”

Section: ------------------------------------------------------------mentioning

confidence: 99%

Grumixama (Eugenia brasiliensis Lam.) cultivated in the Cerrado has high content of bioactive compounds and great antioxidant potential

Alves‐Santos

Dias

et al. 2020

Cienc. Rural

View full text Add to dashboard Cite

Grumixama (Eugenia brasiliensis Lam.) is a Brazilian berry native to the Atlantic Rainforest. Information on nutritional and bioactive profiles of the fruit cultivated in the Brazilian Savannah (Cerrado) is not available in literature. The aim of this study was to investigate the physical and proximate composition; mineral, total phenolics, total tannins and total carotenoids contents; and antioxidant capacity (DPPH and FRAP assays) of the dark purple grumixama cultivated in the Cerrado area (Goiás State). Results showed a similar physical and proximate composition to those of grumixama native to the Atlantic Rainforest. However, grumixama from Cerrado presented the highest dietary fiber and magnesium contents. In addition, the Cerrado grumixama showed higher content of total phenolics (with a large amount of tannins), carotenoids and antioxidant capacity than those of grumixama native to the Atlantic Rainforest, cherry, blueberry, and fruits native to the Cerrado. Thus, the consumption of whole fruit (peel, pulp and seed) in healthy diets and its use as an ingredient for functional food products should be encouraged.

show abstract

Bioprospection of Eugenia brasiliensis, a Brazilian native fruit, as a source of anti-inflammatory and antibiofilm compounds

Cited by 53 publications

References 44 publications

Galleria mellonella as a consolidated in vivo model hosts: New developments in antibacterial strategies and novel drug testing

Galleria mellonella as a consolidated in vivo model hosts: New developments in antibacterial strategies and novel drug testing

Antibacterial activity and mode of action of acetone crude leaf extracts of under-investigated Syzygium and Eugenia (Myrtaceae) species on multidrug resistant porcine diarrhoeagenic Escherichia coli

Grumixama (Eugenia brasiliensis Lam.) cultivated in the Cerrado has high content of bioactive compounds and great antioxidant potential

Contact Info

Product

Resources

About