Bioprospection of cellulolytic and lipolytic South Atlantic deep-sea bacteria

Abstract

Background: Cellulases and lipases have broad industrial application, which calls for an urgent exploration of microorganisms from extreme environments as valuable source of commercial enzyme. In this context, the present work describes the bioprospection and identification of deep-sea bacteria that produce cellulases and lipases, as well their optimal temperature of activity. Results: The first step of this study was the screening of cellulolytic and lipolytic deep-sea bacteria from sediment and water column, which was conducted with substrates linked with 4-Methylumbelliferyl. Among the 161 strains evaluated, 40 were cellulolytic, 23 were lipolytic and 5 exhibited both activities. Cellulolytic and lipolytic bacteria are more common in sediment than at the water column. Based on the ability to produce cellulases and lipases three isolates were selected and identified (16S rRNA sequencing) as Bacillus stratosphericus, B. aerophilus and B. pumilus. Lipases of strain B. aerophilus LAMA 582 exhibited activity at a wide temperature range (4º to 37ºC) and include psychrophilic behaviour. Strain Bacillus stratosphericus LAMA 585 can growth in a rich (Luria Bertani) and minimal (Marine Minimal) medium, and does not need an inducer to produce its mesophilic cellulases and lipases. Conclusions: Deepsea sediments have great potential for bioprospection of cellulase and lipase-producing bacteria. The strains LAMA 582 and LAMA 585 with their special features, exhibit a great potential to application at many biotechnology process.

Effect of Marine Microorganisms on Limestone as an Approach for Calcareous Soil

“…On the other hand, Muñoz [27] isolated 17 strains with autotrophic capabilities, which implies the possibility of metabolizing inorganic molecules by synthesis or hydrolysis. Likewise, Jussie et al [28] isolated 161 strains from the South Atlantic Ocean, of which 58 had cellulolytic and/or proteolytic properties. There are reports of microbial concentrations reaching up to 3.63 × 10 4 microbial cells per milliliter in the Colombian marine muddy snows [29].…”

Caracterización de bacterias cultivables aisladas de sitios de arrecifes mesofóticos de Cuba

“…Entre los grupos fenotípicos más representados para ambos niveles de los sitios mesofóticos se destacan los bacilos G+ esporulados; donde se incluye el género Bacillus, reconocido como productores de diferentes exoenzimas (Odisi et al 2012;Lailaja & Chandrasekaran, 2013;Gawas et al 2019;Jin et al 2019).…”

“…La literatura especializada refiere que las especies de este género son productores de sustancias biológicamente activas, entre las que se encuentran antimicrobianos, tensioactivos, enzimas hidrolíticas y degradadoras de los hidrocarburos del petróleo (Sardessai & Bhosle¸ 2002;Odisi et al 2012).…”