Bioprospecting potential of microbial communities in solid waste landfills for novel enzymes through metagenomic approach

Community diversity metrics, interactions, and metabolic functions of bacteria associated with municipal solid waste landfills at different maturation stages

“…This landfill depth was dominated by phyla Firmicutes, Epsilonbacteraeota, Proteobacteria, and Bacteroidetes in both the 14-and 36-year-old landfills. Several studies have also indicated the dominant presence of Firmicutes, Proteobacteria, and Bacteroidetes in different maturing landfills (Gomez et al, 2011;He et al, 2014;Song et al, 2015;Thakur et al, 2020;Wang et al, 2017;Xu et al, 2017). These mixotrophic phyla are among the most metabolically diverse bacterial groups that metabolize a variety of organic and inorganic substrates (Aislabie & Deslippe, 2013;Vetrovsky, Steffen, & Baldrian, 2014;Ximenes, Cowie, & Barlaz, 2018), as such are functionally key in the degradation of the heterogeneous MSW in landfills.…”

“…Due to their heterogeneous nature, MSW landfills present an intricate and challenging environment for the investigation of in situ microbial dynamics and interactions. For the most part, studies in MSW landfills have been limited to bacterial identification, especially at the phylum level, with the majority of studies reporting Firmicutes , Proteobacteria , Bacteroidetes , and Actinobacteria as most dominant phyla (He et al, 2014; Mwaikono et al, 2016; Song et al, 2015; Stanley, de los Reyes, & Barlaz, 2011; Thakur et al, 2020; Xu et al, 2017; Zainun & Simarani, 2018). However, identification at the genera level has revealed a greater diversity of the bacterial populations from one landfill to another as highlighted in a review by Sekhohola‐Dlamini and Tekere (2019).…”

“…However, identification at the genera level has revealed a greater diversity of the bacterial populations from one landfill to another as highlighted in a review by Sekhohola-Dlamini and Tekere (2019). Nonetheless, recently published literature indicates that there still exists a significant population of uncharacterized microorganisms in MSW landfills (Thakur et al, 2020). It is therefore pertinent for further research to focus on unraveling microbial populations of landfills, particularly at lower levels of classification.…”

Metagenomic Analysis of Microbial Communities From Acidic Marshland Habitat of India

“…For degradation of xenobiotic compounds, novel genes related to pathways can be identi ed. These potential microbes and genes could be used for the purpose of bioremediation, biodegradation and isolation of potential enzymes and their use at an industrial scale [8].The soil sample were obtained from the Pallikaranai wetland which is a freshwater marsh in the city of Chennai, India (12°56′15.72″N 80°12′55.08″E). Metagenomic DNA was extracted from the soil sample by DNeasy PowerMax Soil Kit (Qiagen, Germany) according to the manual instructions [10].…”

Control of synthetic microbial consortia in time, space, and composition

“…In natural biological systems these are more often referred to as microbial communities, and such systems make up the vast majority of all microbial systems on the planet [11]. Microbial consortia have massive impacts on human society through direct interactions, such as the gut microbiome [12], and indirect interactions, such as the spread of pathogens, waste degradation, and agriculture [13][14][15]. Indeed, control of these natural communities is key to the modern world in biotechnological applications such as wastewater treatment [16].…”