Bioprospecting potential and secondary metabolite profile of a novel sediment-derived fungus <i>Penicillium</i> sp. ArCSPf from continental slope of Eastern Arabian Sea

Farha, Arakkaveettil Kabeer; Hatha, A. A. Mohamed

doi:10.1080/21501203.2019.1572034

Cited by 23 publications

(12 citation statements)

References 22 publications

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“…Though there are reports of secondary metabolites with antitumor activity from Penicillium sp. (Bladt et al 2013;Chavez et al 2015;Nicoletti and Trincone 2016;Feng et al 2018;Farha and Hatha 2019;Orfali and Perveen 2019), a metabolite from P. rubens having promising anticancer properties has been reported for the first time in this present work.…”

Section: Discussionmentioning

confidence: 53%

Modulation of Bax and Bcl-2 genes by secondary metabolites produced byPenicillium rubensJGIPR9 causes the apoptosis of cancer cell lines

Venkatachalam

Nadumane

2019

Mycology

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Search for an efficient anti-cancer compound of natural origin with well-defined mechanisms of action is an important scientific pursuit today, due to cancer being the second leading cause for the death of affected people. The members of the genus Penicillium are one of the important sources of bioactive compounds. In the present study, Penicillium rubens, isolated from a garden soil in Madurai district of Tamil Nadu, was found to produce a highly promising anti-cancer metabolite. The percentage viabilities of HepG2, HeLa and MCF-7 cancer cells treated with the bioactive fraction (P5) isolated from P. rubens, ranged between 40-50% after 96 h. Apoptosis induction was found to be the major reason for the observed reduction in cancer cell proliferation and cell count which was confirmed by caspase activity, DNA fragmentation, clonogenic assay, cell cycle analysis and LDH assays. The upregulation of proapoptotic Bax, coupled with the downregulation of anti-apoptotic Bcl-2 expressions were confirmed by RT-qPCR and flow cytometry methods. The current study also indicated an upregulation of p53 which further strengthened the apoptogenic property of P5 fraction. Non-toxicity of P5 was demonstrated on normal peripheral lymphocytes. The analysis of P5 fraction through GC-MS indicated the presence of indole-2, 3-(4,4-dimethyl-3-thiosemicarbazone) as one of the major compounds. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 53%

Modulation of Bax and Bcl-2 genes by secondary metabolites produced byPenicillium rubensJGIPR9 causes the apoptosis of cancer cell lines

Venkatachalam

Nadumane

2019

Mycology

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“…Representatives of the genera Aspergillus, Penicillium and Cladosporium are frequently retrieved in marine habitats, from coastal waters to the deep biosphere, for example, hydrothermal vents and deep subseafloor sediments [ 8 , 11 , 21 ]. Such taxa appear to be of great interest in terms of biotechnological potential thanks to their abilities to produce a diversity of secondary metabolites, including antimicrobial compounds [ 22 , 23 , 24 , 25 ]. Compared with Aspergillus , Penicillium and Cladosporium, marine members of the genera Microscypha and Sistotrema appear to be more limited in their diversity of secondary metabolites produced [ 26 ].…”

Section: Resultsmentioning

confidence: 99%

Highlighting the Biotechnological Potential of Deep Oceanic Crust Fungi through the Prism of Their Antimicrobial Activity

et al. 2021

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Among the different tools to address the antibiotic resistance crisis, bioprospecting in complex uncharted habitats to detect novel microorganisms putatively producing original antimicrobial compounds can definitely increase the current therapeutic arsenal of antibiotics. Fungi from numerous habitats have been widely screened for their ability to express specific biosynthetic gene clusters (BGCs) involved in the synthesis of antimicrobial compounds. Here, a collection of unique 75 deep oceanic crust fungi was screened to evaluate their biotechnological potential through the prism of their antimicrobial activity using a polyphasic approach. After a first genetic screening to detect specific BGCs, a second step consisted of an antimicrobial screening that tested the most promising isolates against 11 microbial targets. Here, 12 fungal isolates showed at least one antibacterial and/or antifungal activity (static or lytic) against human pathogens. This analysis also revealed that Staphylococcus aureus ATCC 25923 and Enterococcus faecalis CIP A 186 were the most impacted, followed by Pseudomonas aeruginosa ATCC 27853. A specific focus on three fungal isolates allowed us to detect interesting activity of crude extracts against multidrug-resistant Staphylococcus aureus. Finally, complementary mass spectrometry (MS)-based molecular networking analyses were performed to putatively assign the fungal metabolites and raise hypotheses to link them to the observed antimicrobial activities.

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“…was shown [35]. Antibacterial and anticancer activity of a sediment-derived fungus fraction that contains oleamide was also identified [36].…”

Section: Discussionmentioning

confidence: 96%

Isolation, identification, and cytotoxicity evaluation of phytochemicals from chloroform extract of Spathodea campanulata

Wagh

Butle

Raut³

2021

Futur J Pharm Sci

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Background Spathodea campanulata P. Beauv. known as the African tulip tree has potential medicinal properties that have been shown traditionally for the treatment of various ailments. The aim of the present study was isolation, identification, and evaluation of the cytotoxic activity of phytochemicals from the chloroform extract of S. campanulata. Result Three compounds were isolated by using column chromatography and preparative TLC from chloroform extract of leaves of S. campanulata. The structures of the isolated compounds were elucidated by using spectroscopic methods, including, FTIR, ESI-TOF MS, 1H NMR, and 13C NMR spectroscopy. In vitro cytotoxic activity of compounds was evaluated by using SRB assay against human leukemia cancer cell lines (HL-60). Results were expressed in IC50 values. Stigmasta-5,22-dien-3-ol, octadecenamide, and umbelliferone were isolated and identified from chloroform extract. The isolated compounds showed cytotoxicity with decreasing cell viability in a dose-dependent manner, but it was found low as compared to positive control, i.e., Adriamycin against HL-60 cell lines. Conclusion The results indicate that isolated compounds, i.e., stigmasta-5,22-dien-3-ol (44.12μg/ml), octadecenamide (35.65μg/ml), and umbelliferone (80.60μg/ml) showed antiproliferative activity, but it was low compared to positive control Adriamycin (10.09 μg/ml). Also, according to our knowledge, this study is the first report on the isolation and identification of octadecenamide and umbelliferone from the leaves of S. campanulata. Graphical abstract

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Bioprospecting potential and secondary metabolite profile of a novel sediment-derived fungus Penicillium sp. ArCSPf from continental slope of Eastern Arabian Sea

Cited by 23 publications

References 22 publications

Modulation of Bax and Bcl-2 genes by secondary metabolites produced byPenicillium rubensJGIPR9 causes the apoptosis of cancer cell lines

Modulation of Bax and Bcl-2 genes by secondary metabolites produced byPenicillium rubensJGIPR9 causes the apoptosis of cancer cell lines

Highlighting the Biotechnological Potential of Deep Oceanic Crust Fungi through the Prism of Their Antimicrobial Activity

Isolation, identification, and cytotoxicity evaluation of phytochemicals from chloroform extract of Spathodea campanulata

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