Bioprospecting of wild type ethanologenic yeast for ethanol fuel production from wastewater-grown microalgae.

Abstract

Background: Wild type yeasts have been successfully used to obtain food products, yet their full potential as fermenting microorganisms for large-scale ethanol fuel production has to be determined. In this study, wild type ethanologenic yeasts isolated from a secondary effluent were assessed for their capability to ferment saccharified microalgae sugars. Results: Yeast species in wastewater were identified sequencing the Internal Transcribed Spacers 1 and 2 regions of the ribosomal cluster. Concurrently, microalgae biomass sugars were saccharified via acid hydrolysis, producing 5.0 ± 0.3 g L-1 of fermentable sugars. Glucose consumption and ethanol production of yeasts in hydrolyzed-microalgae liquor were tested at different initial sugar concentrations and fermentation time. The predominant ethanologenic yeast species was identified as Candida sp., and glucose consumption for this strain and S. cerevisiae achieved 75% and 87% of the initial concentration at optimal conditions, respectively. Relatively similar ethanol yields were determined for both species, achieving 0.45 ± 0.05 (S. cerevisiae) and 0.46 ± 0.05 g ethanol per g glucose (Candida sp.). Conclusion: Overall, the results provide a first insight of the fermentation capacities of specific wild type Candida species, and their potential role in ethanol industries seeking to improve their cost-efficiency.

Direct biohydrogen production from chitosan harvested microalgae biomass and an isolated yeast

“…The yeast Candida sp. for biohydrogen assays was genetically characterized in a previous study detailing [35].…”

Análise do uso de Biocombustíveis como Fonte Alternativa para Combustíveis Fósseis

“…No caso dos alimentos, principalmente após a guerra em curso entre a Rússia e a Ucrânia, é necessário antecipar uma futura escassez, ainda mais que a questão da fome no mundo é um tema que está presente há séculos e tende a piorar. Portanto, avanços tecnológicos e científicos são a chave para contornar qualquer problemática que venha a surgir, bem como acelerar esse uso de energia limpa no mundo [51][52][53][54][55][56][57][58][59][60].…”