Bioprospecting of Microbes for Valuable Compounds to Mankind

Mutalib, Nurul Syakima Ab; Wong, Sunny H.; Ser, Hooi‐Leng; Duangjai, Acharaporn; Law, Jodi Woan‐Fei; Ratnakomala, Shanti; Tan, Loh Teng‐Hern; Letchumanan, Vengadesh

doi:10.36877/pmmb.a0000088

Cited by 32 publications

(30 citation statements)

References 168 publications

(186 reference statements)

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“…The abundance of species can be the root of the enormous supply of structurally diverse natural products. By harvesting these compounds from microbial sources, it will be relatively cost-efficient compared to the chemical synthesis approach [ 36 , 37 , 38 ]. As a result, many microorganisms have been screened for the production of anticancer compounds/leads.…”

Section: Microbial Sources: Why the Streptomyces mentioning

confidence: 99%

Anticancer Drug Discovery from Microbial Sources: The Unique Mangrove Streptomycetes

Law

Letchumanan

et al. 2020

Molecules

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Worldwide cancer incidence and mortality have always been a concern to the community. The cancer mortality rate has generally declined over the years; however, there is still an increased mortality rate in poorer countries that receives considerable attention from healthcare professionals. This suggested the importance of the prompt detection, effective treatment, and prevention strategies. The genus Streptomyces has been documented as a prolific producer of biologically active secondary metabolites. Streptomycetes from mangrove environments attract researchers’ attention due to their ability to synthesize diverse, interesting bioactive metabolites. The present review highlights research on mangrove-derived streptomycetes and the production of anticancer-related compounds from these microorganisms. Research studies conducted between 2008 and 2019, specifically mentioning the isolation of streptomycetes from mangrove areas and described the successful purification of compound(s) or generation of crude extracts with cytotoxic activity against human cancer cell lines, were compiled in this review. It is anticipated that there will be an increase in prospects for mangrove-derived streptomycetes as one of the natural resources for the isolation of chemotherapeutic agents.

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Section: Microbial Sources: Why the Streptomyces mentioning

confidence: 99%

Anticancer Drug Discovery from Microbial Sources: The Unique Mangrove Streptomycetes

Law

Letchumanan

et al. 2020

Molecules

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“…Bacteria and bacterially produced bioactive compounds have recently emerged as a promising alternative for cancer therapeutics [ 30 ]. Bacteria like Actinobacteria are a particularly rich source of compounds with antimicrobial, anticancer, antioxidant, neuroprotective, enzymatic, and immunosuppressive functions [ 31 ]. Bacterial anticancer agents such as antibiotics, bacteriocins, non-ribosomal peptides, and polyketides showed different mechanisms of actions such as apoptosis, necrosis, reduced angiogenesis, inhibition of translation, and splicing and obstructing essential signaling pathways to kill cancer cells [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Streptomyces griseus KJ623766: A Natural Producer of Two Anthracycline Cytotoxic Metabolites β- and γ-Rhodomycinone

et al. 2021

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Background: This study aimed to produce, purify, structurally elucidate, and explore the biological activities of metabolites produced by Streptomyces (S.) griseus isolate KJ623766, a recovered soil bacterium previously screened in our lab that showed promising cytotoxic activities against various cancer cell lines. Methods: Production of cytotoxic metabolites from S. griseus isolate KJ623766 was carried out in a 14L laboratory fermenter under specified optimum conditions. Using a 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium-bromide assay, the cytotoxic activity of the ethyl acetate extract against Caco2 and Hela cancer cell lines was determined. Bioassay-guided fractionation of the ethyl acetate extract using different chromatographic techniques was used for cytotoxic metabolite purification. Chemical structures of the purified metabolites were identified using mass, 1D, and 2D NMR spectroscopic analysis. Results: Bioassay-guided fractionation of the ethyl acetate extract led to the purification of two cytotoxic metabolites, R1 and R2, of reproducible amounts of 5 and 1.5 mg/L, respectively. The structures of R1 and R2 metabolites were identified as β- and γ-rhodomycinone with CD50 of 6.3, 9.45, 64.8 and 9.11, 9.35, 67.3 µg/mL against Caco2, Hela and Vero cell lines, respectively. Values were comparable to those of the positive control doxorubicin. Conclusions: This is the first report about the production of β- and γ-rhodomycinone, two important scaffolds for synthesis of anticancer drugs, from S. griseus.

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“…Harnessing the genomic potential of microbes, including Streptomyces sp. is indeed one way forward in drug discovery [78][79][80] . Combined with the conventional methods, including optimization of fermentation media and improvement in extraction processes, the activation of biosynthetic gene clusters via heterologous expression or introduction of promoters in the native host can potentially yield bioactive compound(s) of interest in high purity and quantity, allowing its large-scale production for pharmaceutical use [81][82][83][84][85] .…”

Section: Data Descriptionmentioning

confidence: 99%

Whole-genome sequence of bioactive streptomycete derived from mangrove forest in Malaysia, Streptomyces sp. MUSC 14

Ser¹,

Tan²,

Tan³

et al. 2021

Prog Micobes Mol Biol

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The contribution of streptomycetes to human health is undeniably important and significant, given that these filamentous microbes can produce interesting compounds that can be used to cure deadly infections and even cancer. Isolated from the east coast of Peninsular Malaysia, Streptomyces sp. MUSC 14 has shown significant antioxidant capacity. The current study explores the genomic potential of MUSC 14 via a genome mining approach. The genome size of MUSC 14 is 10,274,825 bp with G + C content of 71.3 %. AntiSMASH analysis revealed a total of nine biosynthetic gene clusters (with more than 80 % similarities to known gene clusters). This information serves as an important foundation for subsequent studies, particularly the purification and isolation of bioactive compounds by genetic manipulation techniques.

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Bioprospecting of Microbes for Valuable Compounds to Mankind

Cited by 32 publications

References 168 publications

Anticancer Drug Discovery from Microbial Sources: The Unique Mangrove Streptomycetes

Anticancer Drug Discovery from Microbial Sources: The Unique Mangrove Streptomycetes

Streptomyces griseus KJ623766: A Natural Producer of Two Anthracycline Cytotoxic Metabolites β- and γ-Rhodomycinone

Whole-genome sequence of bioactive streptomycete derived from mangrove forest in Malaysia, Streptomyces sp. MUSC 14

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