Bioprospecting from marine coastal sediments of Colombian Caribbean: screening and study of antimicrobial activity

Abstract

These results contribute to the knowledge of the biotechnological potential of the Colombian biodiversity for the development of pharmaceutical products that can counteract the increasing problem of pathogen resistance.

In vitro biological activity of extracts from marine bacteria cultures against Toxoplasma gondii and Mycobacterium tuberculosis

“…Bioprospecting in marine unexplored environments such as the deep‐sea can accelerate drug discovery. Earlier work from our group isolated several bacteria from the Colombian Caribbean Sea (Quintero et al, 2018; Santos‐Acevedo et al, 2018). In this study, seven strains isolated from estuarine, marine, coastal and deep‐sea were screened with the aim to evaluate the biological activity against T. gondii and M. tuberculosis to identify the most promissory bacteria.…”

“…Two millilitres of each suspension were placed in 50 ml of production medium (Table S1) and incubated for 72 h at 30°C in orbital shaker (Thermo Scientific™) at 140 rpm. From this initial culture, 8 ml were used for inoculation of 200 ml of production medium, incubated in same conditions for 168 h, with the exception of the strains INV PRT33 and INV FIR35, which were maintained for 72 and 24 h, respectively, according to the antimicrobial activity report in a previous study (Quintero et al, 2018). The cultures were centrifuged at 2706 g at 4°C for 30 min (Hettich centrifuge 320R), and the supernatants were filtered with 0.20 µm pore sterile syringe filters (Sartorius™).…”

“…In Colombia, microbial bioprospection has been mainly focused on terrestrial environments, whereas those organisms that inhabit marine environment have been little explored, and particularly the extremophiles found in fumaroles, hot springs, coastal salt lagoons and deep sea pits are of interest (Gómez et al, 2000). Recent reports have focused on bacteria isolated from marine sediments, to detect antimicrobial activity (Quintero et al, 2018), biofilm formation (López et al, 2019), biosurfactant production (Santos‐Acevedo et al, 2018), polyhydroxyalkanoate production (Blandón et al, 2020) and hydrocarbon degradation potential (Narváez‐Flórez et al, 2008).…”

Marine Sediment-Derived Streptomyces Strain Produces Angucycline Antibiotics against Multidrug-Resistant Staphylococcus aureus Harboring SCCmec Type 1 Gene

“…Efforts in searching for bioactive compounds from marine sediment-derived actinomycetes gained the attention of many researchers in the last five years [7,12,[37][38][39][40][41][42][43][44]. Some examples of marine actinomycetes that produce bioactive compounds include the marine sediment-derived Streptomyces sp.…”

Diversity, Ecology, and Prevalence of Antimicrobials in Nature

“…These compounds can be toxic to the surrounding community, providing a selective advantage for nutrients, carbon, and space (Bibb, 2005; Rigali et al, 2008). Many environments have been explored for novel antimicrobial discovery including continental soils (Wright, 1956; Gottlieb, 1976; Chander et al, 2005; Bundale et al, 2015), caves (Cheeptham and Saiz-Jimenez, 2015), desert soils (Hozzein et al, 2011; El-Deeb et al, 2013; Nithya et al, 2015; Ouchari et al, 2019), freshwater sediments and water (Cross, 1981; Cannell et al, 1988; Madhumathi et al, 2011), and marine sediments and water (Duff et al, 1966; Martins et al, 2008; Valli et al, 2012; Bose et al, 2015; Zinke et al, 2017; Quintero et al, 2018; Ser et al, 2018; Hook and Plante, 2019; Skočibušić et al, 2019). Terrestrial areas including soils, deserts, and freshwater lakes and rivers are typically easier to access relative to deep-sea environments and generate promising results in the hunt for antimicrobial activity, as natural products isolated from these environments have been used medicinally (Mcdonald et al, 1996; Haefner, 2003; Hughes et al, 2008; Dias et al, 2012; David et al, 2015).…”