Bioprospecting for culturable actinobacteria with antimicrobial properties isolated from rivers in Colombian Orinoquia

Pastrana-Camacho, Natalia; Suárez, Zulma; Acosta-González, Alejandro; Arango, Carolina; Haltli, Brad; Correa, Hebelin; Kerr, Russel; Duque, Carmenza; Díaz, Luis Eduardo

doi:10.4314/tjpr.v15i6.19

Cited by 9 publications

(19 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Petri dishes were incubated in an Innova ® 42 Incubator at 37 o C for 24 h and bacterial growth against the actinobacterial isolate seeded, was observed. Antibiosis was indicated by visually observable growth inhibition on target organisms and results were recorded as follows: inhibition zone > 2 cm: +++ (good activity), inhibition zone between 1-2 cm: ++ (moderate activity), inhibition zone < 1 mm: + (weak activity) and – (no activity) [ 47 ]. Negative and positive controls using rifampicin, vancomycin, ciprofloxacin and nalidixic acid (50mg/mL) (Sigma-Aldrich ® ) for pathogenic bacteria were assessed in a petri dish with Muller-Hinton agar (MH-Oxoid ® ) and were incubated in an Innova ® 42 Incubator at 37 o C for 24h.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Actinobacterial Communities from Arauca River Sediments (Colombia) Reveals Antimicrobial Potential Presented in Low Abundant Isolates

Arango¹,

Acosta-González²,

Parra-Giraldo³

et al. 2018

TOMICROJ

Self Cite

View full text Add to dashboard Cite

Introduction:New strategies have been arisen to set a rapid and effective screening for selection of microorganism with bioactive potential. This study suggests that combination of physicochemical pretreatments and taxonomic dereplication of microbial collections through MALDI-TOF MS, facilitates the detection of low abundance actinobacteria with potential as a source of antimicrobial agents.Material and Methods:An unstudied microbial community from a tropical river sediment in Colombian Orinoquía is described, applying an extended cultivation strategy using physicochemical pretreatments, biological screenings and taxonomic dereplication through MALDI-TOF MS approach.Results:Actinobacteria-like isolates (790) were growth and their antimicrobial activity was assessed against methicillin-resistant Staphylococcus aureus, Vancomycin-resistant Enterococcus faecium, extended-spectrum β-lactamase Klebsiella pnumoniae, and clinical isolates of Cladosporium cladosporioides and Epicoccum nigrum. Seventy-eight isolates, belonging to the Streptomycetaceae family according to 16S rDNA analysis were found to have antimicrobial activity and were categorized as low abundance actinobacteria by MALDI-TOF MS.Conclusion:The results suggest that combination of physicochemical pretreatments and taxonomic dereplication of microbial collections through MALDI-TOF MS, facilitates the detection of low abundance actinobacteria with potential as a source of antimicrobial agents.

show abstract

Section: Methodsmentioning

confidence: 99%

Characterization of Actinobacterial Communities from Arauca River Sediments (Colombia) Reveals Antimicrobial Potential Presented in Low Abundant Isolates

Arango¹,

Acosta-González²,

Parra-Giraldo³

et al. 2018

TOMICROJ

Self Cite

View full text Add to dashboard Cite

show abstract

“…Taking into account that the production of L-asparaginase is influenced by physical and chemical factors [26], six carbon sources at 1% (lactose, xylose, ribose, inositol and maltose) were evaluated in twenty-five selected strains with high production of L-asparaginase [21,26,27]. Glucose (1%) was used as a positive control and medium without carbon source as a negative control.…”

Section: Determination Of the Effect Of The Carbon And Nitrogen Sourcmentioning

confidence: 99%

“…Glucose (1%) was used as a positive control and medium without carbon source as a negative control. Likewise, the effect of nitrogen source at 0.05% (meat extract, malt extract and peptone in combination with L-asparagine (0.05%)) [21,26,27] was evaluated. Lasparagine (0.1%) was used as a positive control and medium without a nitrogen source as a negative control.…”

Section: Determination Of the Effect Of The Carbon And Nitrogen Sourcmentioning

confidence: 99%

Production and Characterization of L-Asparaginases of Streptomyces Isolated from the Arauca Riverbank (Colombia)

Arévalo-Tristancho¹,

Díaz²,

Cortázar³

et al. 2019

TOMICROJ

View full text Add to dashboard Cite

Introduction: L-asparaginase, is known as an anti-cancer agent, mainly used in acute lymphoblastic leukemia, which prevents the proliferation of tumor cells. This study shows that there are unexplored regions in Colombia that can be sources of obtaining this enzyme and that the optimization of the production of L-asparaginase from native isolates can be determined in the search for alternatives to commercial drugs. Materials and Methods: Selection and identification of Streptomyces among Actinobacteria isolated from the Arauca riverbank for L-asparaginase producers are described. In addition, the effect of carbon and nitrogen sources, pH, temperature and agitation rate are studied for L-asparaginase activity in liquid culture using Plackett-Burman design and Taguchi methodology. Kinetic characterization of a purified L-asparaginase and its cytotoxic potential are evaluated too. Results: Seven of seventy-eight actinobacterial strains were selected as L-asparaginase producing Streptomyces showing a high L-asparaginase/L-glutaminase ratio in liquid culture with lactose as substrate. The strain 112 identified as Streptomyces lacticiproducens was chosen for L-asparaginase production at these culture conditions: lactose 0.25%, L-asparagine 0,015%, malt extract 0,015%, pH 7.36, 32°C and 130 rpm. Enzymatic studies of the purified L-asparaginase showed that the optimal pH and temperature were 6 and 37.5°C, respectively. This purified enzyme had an IC50 of 36.74 µg/mL on THP-1 cells. Conclusion: S. lacticiproducens isolated from the Arauca riverbank is a new source for the production of a high activity L-asparaginase, creating expectation of its availability as a drug for the acute lymphoblastic leukemia treatment.

show abstract

“…The strains analyzed belonged to the Actinobacteria biobank of La Sabana University and were isolated from sediments of the Guaviare river [18].…”

Section: Biological Materialsmentioning

confidence: 99%

“…The 16S rRNA gene was amplified using polymerase chain reaction (PCR) in a thermocycler (BIO-RAD iCycler, USA) with extracted DNA as the template [18]. The PCR reaction was confirmed by electrophoresis in 1× Tris-Borate-EDTA (TBE) buffer and compared with a ladder of molecular markers (HyperLadder™ IV, Bioline, London).…”

Section: Molecular Identification Of Actinobacteria Strainsmentioning

confidence: 99%

Optimization of L-asparaginase activity of Actinobacteria isolated from Guaviare river sediments in Colombia

Morales-González¹,

Martinez²,

Ramírez-Rodríguez³

et al. 2019

Trop. J. Pharm Res

View full text Add to dashboard Cite

Purpose: To optimize the L-asparaginase activity of Actinobacteria isolated from Guaviare river sediments in Colombia. Methods: Actinobacterial strains were evaluated for their L-asparaginase activity using phenol red plates and Nessler's assays. Strains with L-asparaginase activity were identified based on 16S ribosomal rRNA sequencing, and a central composite design was used to study nutritional and growth factors that could improve L-asparaginase activity. L-asparaginase protein was detected using western blotting and the cytotoxicity of L-asparaginase preparations was evaluated against MDA-MB231 and L929 cell lines. Results: Kitasatospora atroaurantiaca, Streptomyces griseoluteus, and Streptomyces panaciradicis were cultured in medium with lactose as a carbon source and a combination of asparagine and malt extract as nitrogen sources.

show abstract

Bioprospecting for culturable actinobacteria with antimicrobial properties isolated from rivers in Colombian Orinoquia

Cited by 9 publications

References 26 publications

Characterization of Actinobacterial Communities from Arauca River Sediments (Colombia) Reveals Antimicrobial Potential Presented in Low Abundant Isolates

Characterization of Actinobacterial Communities from Arauca River Sediments (Colombia) Reveals Antimicrobial Potential Presented in Low Abundant Isolates

Production and Characterization of L-Asparaginases of Streptomyces Isolated from the Arauca Riverbank (Colombia)

Optimization of L-asparaginase activity of Actinobacteria isolated from Guaviare river sediments in Colombia

Contact Info

Product

Resources

About