Bioprospecting foliar endophytic fungi of Vitis labrusca Linnaeus, Bordô and Concord cv.

Felber, Aretusa Cristina; Orlandelli, Ravely Casarotti; Rhoden, Sandro Augusto; García, Adriana; Costa, Alessandra Tenório; Azevedo, João Lúcio de; Pamphile, João Alencar

doi:10.1007/s13213-015-1162-6

Cited by 19 publications

(25 citation statements)

References 37 publications

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“…Fourteen fungal strains of genera Alternaria, Bipolaris, Cochliobolus, Diaporthe, Fusarium and Phoma (Table 1) were used. Fungi were isolated from healthy leaves of Bordô and Concord cultivars of V. labrusca (FELBER et al, 2016) and belong to the fungal culture collection of the Laboratório de Biotecnologia Microbiana, Universidade Estadual de Maringá, Maringá PR Brazil. Molecular identification was based on the sequencing of ITS1-5.8S-ITS2 region of rDNA.…”

Section: Endophytic Fungimentioning

confidence: 99%

“…Molecular identification was based on the sequencing of ITS1-5.8S-ITS2 region of rDNA. Sequences were deposited in the GenBank database (FELBER et al, 2016).…”

Section: Endophytic Fungimentioning

confidence: 99%

“…Endophytic fungi colonize intra-or intercellular spaces of healthy plant tissues (FELBER et al, 2016;STONE et al, 2000) without causing any apparent damage; they are ubiquitous organisms found inside all plants (RODRIGUEZ et al, 2009). During their long coexistence process with host plants, these fungi developed important characteristics to maintain a stable symbiosis.…”

Section: Introductionmentioning

confidence: 99%

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Endoglucanase production by endophytic fungi isolated from Vitis labrusca L. with peanut hull and sawdust as substrates

Felber¹,

Specian²,

Orlandelli³

et al. 2019

Biosci. J.

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Endoglucanases are enzymes widely employed in different industrial fields, albeit with high production costs. Studies on new microbial sources and low-cost substrates are highly relevant, including those on agro-industrial. Current analysis evaluates peanut hull (PH) and sawdust (SD) as substrates for submerged cultures of 14 endophytic fungi isolated from grapevine (Vitis labrusca L.) cultivars Bordô and Concord. Endophytes were grown on a carboxymethylcellulose (CMC) medium and the cup plate assay showed that eight strains (belonging to genera Cochliobolus, Diaporthe, Fusarium and Phoma) had positive results: enzymatic halos ranged from 10.8±0.02 to 15.5±0.07 mm in diameter. Diaporthe sp. strains (GenBank accession codes KM362392, KM362368 and KM362378) and Fusarium culmorum KM362384 were highlighted as the most promising sources. Further, PH and SD as substrates for the fermentation of these fungi were evaluated by the cup plate assay and endoglucanase activity assay. Highest halo diameters were obtained for Diaporthe sp. KM362392: 16.1±0.01 mm (CMC), 14.5±0.01 mm (PH) and 14.7±0.03 mm (SD). The fungus also presented the highest levels of endoglucanase activity: analysis of variance revealed that CMC (3.52±0.98 µmol/min), PH (2.93±0.23 µmol/min) and SD (3.26±0.38 µmol/min) were similarly efficient as substrates. Results deepen knowledge on V. labrusca endophytes that may be endoglucanase sources, even though further optimizations in submerged cultures with PH and SD should be undertaken to increase the enzymatic production from these wastes.

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Section: Endophytic Fungimentioning

confidence: 99%

“…Molecular identification was based on the sequencing of ITS1-5.8S-ITS2 region of rDNA. Sequences were deposited in the GenBank database (FELBER et al, 2016).…”

Section: Endophytic Fungimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Endoglucanase production by endophytic fungi isolated from Vitis labrusca L. with peanut hull and sawdust as substrates

Felber¹,

Specian²,

Orlandelli³

et al. 2019

Biosci. J.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Genomic DNA was extracted according to Felber et al (2015). DNA concentration and integrity were analyzed by electrophoresis in 1% agar gel with Lambda DNA digested by HindIII (Gibco-Invitrogen, Carlsbad, CA, USA) as molecular weight standard.…”

Section: Molecular Identificationmentioning

confidence: 99%

Endophytes isolated from passion fruit plants: molecular identification, chemical characterization and antibacterial activity of secondary metabolites

2017

J App Pharma Sci

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Endophytic fungi inhabit the interior of healthy plants without any apparent harm and may synthesize bioactive compounds, which constitute an alternative for the control of human pathogens. Current study reports the molecular identification of two endophytes from passion fruit plants: isolate PE1-13 (from Passiflora edulis f. flavicarpa) has been identified as Phyllosticta sp. and isolate PA8-2 (from Passiflora alata) is a Cercospora beticola strain. Thin layer chromatography showed that steroids and triterpenoids were present in the secondary metabolites produced by the two endophytes. The antibacterial assay against Enterococcus faecalis, Salmonella typhi and Streptococcus pyogenes indicated that the fungal metabolites reduced up to 98.1±0.5% of bacterial growth. Results suggest that these endophytes are capable of producing antibacterial compounds.

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“…In fact, the bulk of FEF research is represented by studies focusing on a specific host plant (Jumpponen & Jones, 2010;Zimmerman & Vitousek, 2012;Mejía et al, 2014;Saucedo-García et al, 2014;Oono et al, 2015;Polonio et al, 2015;Felber et al, 2016;González-Teuber, 2016;Kato et al, 2017). Studies that have surveyed FEF or other leaf-associated fungi across multiple plant species have found significant host effects (Unterseher et al, 2012;Kembel & Mueller, 2014;Massimo et al, 2015;Huang et al, 2016;Vincent et al, 2016), suggesting that host identity likely interacts with the abiotic environment to structure FEF community composition.…”

Section: Introductionmentioning

confidence: 99%

Fungal communities living within leaves of native Hawaiian dicots are structured by landscape-scale variables as well as by host plants

Darcy

Cobian

Swift

et al. 2019

Preprint

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AIM: A phylogenetically diverse array of fungi live within healthy leaf tissue of dicotyledonous plants. Many studies have examined these endophytes within a single plant species and/or at small spatial scales, but landscape-scale variables that determine their community composition are not well understood, either across geographic space, across climatic conditions, or in the context of host plant phylogeny. Here, we evaluate the contributions of these variables to endophyte community composition using our survey of foliar endophytic fungi in native Hawaiian dicots sampled across the Hawaiian archipelago. LOCATION: Hawaiʻi.METHODS: The Hawaiian archipelago offers a uniquely tractable system to study biogeography of foliar endophytic fungi, because the islands harbor a wide array of climatic conditions, and native plant species are often found across wide elevational and climactic ranges. We used Illumina technology to sequence fungal ITS1 amplicons in order to characterize foliar endophyte communities in the leaves of 896 plants across 5 islands and 80 host plant genera. Using Generalized Dissimilarity Modeling (GDM) we tested the effect of landscape-scale variables on observed differences in foliar endophyte communities. Bipartite network analysis was used to examine the extent to which each island harbored specialized or cosmopolitan foliar endophytes.RESULTS: Communities of foliar endophytic fungi in the Hawaiian archipelago are structured most strongly by evapotranspiration, elevation, vegetation/habitat type, and by the phylogeny of host plants. The five islands we sampled each harbored significantly specialized endophyte communities as well.MAIN CONCLUSIONS: Factors that structure foliar endophyte communities at small geographic and narrow host phylogenetic scales are broadly generalizable to the larger scales we studied here, although not universally. Evapotranspiration, a variable with resolution 250 m 2 , was the most robust predictor of endophyte community dissimilarity in our study, although it had not previously been considered an important determinant of FEF communities.

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Bioprospecting foliar endophytic fungi of Vitis labrusca Linnaeus, Bordô and Concord cv.

Cited by 19 publications

References 37 publications

Endoglucanase production by endophytic fungi isolated from Vitis labrusca L. with peanut hull and sawdust as substrates

Endoglucanase production by endophytic fungi isolated from Vitis labrusca L. with peanut hull and sawdust as substrates

Endophytes isolated from passion fruit plants: molecular identification, chemical characterization and antibacterial activity of secondary metabolites

Fungal communities living within leaves of native Hawaiian dicots are structured by landscape-scale variables as well as by host plants

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