Bioproduction, structure elucidation and in vitro antiproliferative effect of eumelanin pigment from Streptomyces parvus BSB49

Bayram, Sinan; Dengiz, Çağatay; Gerçek, Yusuf Can; Çeti̇n, İdi̇l; Topçul, Mehmet

doi:10.1007/s00203-020-01956-2

Cited by 29 publications

(36 citation statements)

References 31 publications

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“…The free radicals and other reactive species are easily scooped up by melanin pigment due to valence electrons in their molecules. They are used as antioxidants to facilitate their use in cosmetic products, which reduces tissue damage due to toxins [ 20 ]. Melanin acts as a potent anticancer agent that kills the cancer cells with low concentrations without causing damage to normal cells.…”

Section: Discussionmentioning

confidence: 99%

“…Extracted melanin pigment obtained above was dissolved in 0.5 M NaOH solution and then analyzed in UV-visible (near-infrared wavelengths of 200–800 nm) spectrophotometer and NaOH (0.5 M) solution was used as blank [ 20 , 24 ].…”

Section: Methodsmentioning

confidence: 99%

“…Water-soluble melanin is widely used in sunscreens, solid plastic films, lenses, lacquer paints, and other formulations to provide greater protection from UV [ 16 ]. Significant biological activities of melanin include protection to liver [ 17 ] antibacterial [ 18 ] antitumor [ 19 ] antioxidant [ 20 ] and anti-inflammatory [ 21 ]. The present study was aimed to extract melanin produced from Streptomyces puniceus RHPR9.…”

Section: Introductionmentioning

confidence: 99%

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Melanin pigment of Streptomyces puniceus RHPR9 exhibits antibacterial, antioxidant and anticancer activities

et al. 2022

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The present study reveals the production of dark, extracellular melanin pigment (386 mg/L) on peptone yeast extract iron agar medium by Streptomyces puniceus RHPR9 using the gravimetric method. UV-Visible, Fourier Transform Infrared (FTIR), and Nuclear Magnetic Resonance (1H) (NMR) spectroscopy confirmed the presence of melanin. Extracted melanin showed antibacterial activity against human pathogens such as Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli except for Klebsiella pneumoniae. A potent free radical scavenging activity was observed at 100 μg/mL of melanin by the DPPH method with a concentration of 89.01±0.05% compared with ascorbic acid 96.16±0.01%. Antitumor activity of melanin was evaluated by MTT assay against HEK 293, HeLa, and SK-MEL-28 cell lines with IC50 values of 64.11±0.00, 14.43±0.02, and 13.31±0.01 μg/mL respectively. Melanin showed maximum anti-inflammatory activity with human red blood cells (hRBC) (78.63 ± 0.01%) and minimum hemolysis of 21.37±0.2%. The wound healing potential of the pigment was confirmed on HeLa cells, cell migration was calculated, and it was observed that cell migration efficiency decreased with an increase in the concentration of melanin. To our knowledge, this is the first evidence of melanin produced from S. puniceus RHPR9 that exhibited profound scavenging, anti-inflammatory and cytotoxic activities.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Melanin pigment of Streptomyces puniceus RHPR9 exhibits antibacterial, antioxidant and anticancer activities

et al. 2022

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“…Bacterial cells produce a huge number of biologically active compounds, including vitamins, amino acids, antibiotics, and enzymes [1]. At present, the biotechnological significance of one more group of secondary metabolites of bacteria, pigments, has been estimated [2]. The ability to synthesize pigments that differ in chemical composition, color, and solubility is characteristic of many microorganisms [3].…”

Section: Introductionmentioning

confidence: 99%

A Blue-Purple Pigment-Producing Bacterium Isolated from the Vezelka River in the City of Belgorod

et al. 2021

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Violacein is a biotechnologically significant secondary metabolite due to its antibacterial, antifungal, and other properties. Isolation, research, and identification of violacein producing strains are of interest for the development of biotechnological processes, in order to enhance the biosynthesis of this compound. The purpose of the present work was to study the properties of a newly isolated bacterium capable of synthesizing blue-purple pigment. An aboriginal bacterium was isolated from the coastal zone of the Vezelka River in the city of Belgorod. Based on chemical and spectrophotometric studies of the crude ethanol extract, the pigment was identified as violacein, and the isolate was assigned to the group of violacein-forming bacteria, which includes bacteria of the genera Chromobacterium, Iodobacter, Janthinobacterium, Duganella, Collimonas, and Massilia. Based on cultural, morphological, tinctorial, physiological, and biochemical properties, as well as analysis of the 16S rRNA gene sequence, the new isolated strain was assigned to the genus Janthinobacterium. The isolated strain is capable of suppressing the growth of a number of fungal and bacterial phytopathogens. For representatives of the genus Janthinobacterium, their inhibitory influence on cyanobacteria was shown for the first time.

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“…The cytotoxicity of Cetuximab and Parp inhibitor (Parp 1 inhibitor) on the cells as a result of the application of scheduled concentrations was researched with the MTT test (18). Absorbance values of the experimental groups were measured by spectrophotometer at 570 nm by taking the 690 nm wavelength as reference.…”

Section: Cell Viabilitymentioning

confidence: 99%

Antiproliferative effects of EGFR inhibitor Cetuximab and parp inhibitor combination on non-small cell lung cancer cell line A549 and cervical cancer cell line HeLa

Çeti̇n

Topçul

2022

Cell Mol Biol (Noisy-le-grand)

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Bioproduction, structure elucidation and in vitro antiproliferative effect of eumelanin pigment from Streptomyces parvus BSB49

Cited by 29 publications

References 31 publications

Melanin pigment of Streptomyces puniceus RHPR9 exhibits antibacterial, antioxidant and anticancer activities

Melanin pigment of Streptomyces puniceus RHPR9 exhibits antibacterial, antioxidant and anticancer activities

A Blue-Purple Pigment-Producing Bacterium Isolated from the Vezelka River in the City of Belgorod

Antiproliferative effects of EGFR inhibitor Cetuximab and parp inhibitor combination on non-small cell lung cancer cell line A549 and cervical cancer cell line HeLa

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