Bioprocessing strategies for cost-effective large-scale production of bacterial laccase from Lysinibacillus macroides LSO using bio-waste

Abdelgalil, Soad A.; Soliman, Nadia A.; Abo-Zaid, Gaber Attia; Abdel-Fattah, Yasser R.

doi:10.1007/s13762-021-03231-3

“…The total genomic DNA of the overnight grown promising bacterial isolate was extracted using the methodology of Marahatta et al 33 . PCR and sequencing were carried out in accordance with the method of Abdelgalil et al 34 .…”

Section: Methodsmentioning

confidence: 99%

Biovalorization of raw agro-industrial waste through a bioprocess development platform for boosting alkaline phosphatase production by Lysinibacillus sp. strain APSO

Abdelgalil

¹

,

Soliman

²

,

Abo-Zaid

³

et al. 2021

Sci Rep

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This study highlighted the exploitation of mathematical models for optimizing the growth conditions that give the highest phosphatase productivity from a newfound Lysinibacillus sp. strain APSO isolated from a slime sample. Mathematical models facilitate data interpretation and provide a strategy to solve fermentation problems. Alkaline phosphatase (ALP) throughput was enhanced by 16.5-fold compared to basal medium based on a sequential optimization strategy that depended on two-level Plackett–Burman design and central composite design. The additional improvement for volumetric productivity and specific production yield was followed in a 7 L bench-top bioreactor to evaluate microbial growth kinetics under controlled and uncontrolled pH conditions. The pH-controlled batch cultivation condition neither supported cell growth nor enhanced ALP productivity. In contrast, the uncontrolled pH batch cultivation condition provided the highest ALP output (7119.4 U L−1) and specific growth rate (µ = 0.188 h−1) at 15 h from incubation time, which was augmented > 20.75-fold compared to the basal medium. To the authors’ knowledge, this study is the second report that deals with how to reduce the production cost of the ALP production process via utilization of agro-industrial waste, such as molasses and food waste (eggshell), as a nutrimental source for the improvement of the newfound Lysinibacillus sp. strain APSO ALP throughput.

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“…Hence, the strain becomes potent producer of the lignocellulosic enzymes utilizing LCAWs as this study is rst to be reported for the high titer of cocktail of lignocellulolytic enzymes production with this strain. However, some authors stated the lignocellulolytic enzymes production using various LCAWs by several fungi [3,5,[11][12] and certain bacteria [10,21]. But none of these authors reported all the lignocellulolytic enzymes reported in this study.…”

Section: Optimization For the Cocktail Of Lignocellulolytic Enzymes Productionmentioning

confidence: 62%

Production of Cocktail of Lignolytic, Cellulolytic and Hemicellulolytic Enzymes by the Novel Bacterium Burkholderia Sp SMB1 Utilizing Rice Bran and Straw: Application in the Saccharification of Untreated Agro-Wastes for Bioethanol Production

Beladhadi

¹

,

Shankar

²

,

Jayalakshmi

³

et al. 2021

Preprint

0

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Purpose: This study focuses to assess the potentiality of the locally isolated Burkholderia sp SMB1. It was aimed for the cocktail of lignocellulolytic enzyme production involved in the depolymerization of the agro-wastes to release sugars for bioethanol production. Methods: The optimization of enzyme cocktail (lignolytic, cellulolytic, hemicellulolytic) production by isolated bacterium utilizing inexpensive substrates like bran and straw of rice was done using response surface methodology. Further this cocktail secreted by the bacterium at optimized conditions was employed for saccharifying untreated agro-wastes for sugars production which were fermented to bioethanol by S. cerevisiae.Results: This strain produced high titer of cellulase (10.8 U/mL), xylanase (76 U/mL), mannanase (14.23 U/mL), pectinase (62.18 U/mL) and laccase (24.25 U/mL) using rice bran at 7 pH and 40 oC using 10% (w/v) of rice bran. The production of cocktail of enzymes was slightly increased by adding CaCO3 (2mM) in the growth medium. This cocktail was able to hydrolyze untreated agro-wastes to release highest reducing sugars 28.5 g/L using untreated corn husk after 24 h of saccharification process. These sugars of corn husk were fermented by S. cerevisiae to produce highest 9.04 gL-1 bioethanol. Conclusion: This paper represents the exploitation of the locally isolated strain for the production of lignocellulolytic enzymes using rice bran and straw for the first time promising the novelty of the study. This study helps in waste disposal process by valorizing the waste biomass to produce value added products like sugars, bioethanol and hence increases agricultural economy.

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“…Hence, the strain becomes potent producer of the lignocellulosic enzymes utilizing LCAWs as this study is rst to be reported for the high titer of cocktail of lignocellulolytic enzymes production with this strain. However, some authors stated the lignocellulolytic enzymes production using various LCAWs by several fungi [3,5,[11][12] and certain bacteria [10,21]. But none of these authors reported all the lignocellulolytic enzymes reported in this study.…”

Section: Optimization For the Cocktail Of Lignocellulolytic Enzymes P...mentioning

confidence: 63%

Production of Cocktail of Lignolytic, Cellulolytic and Hemicellulolytic Enzymes by the Novel Bacterium Burkholderia sp SMB1 Utilizing Rice Bran and Straw: Application in the Saccharification of Untreated Agro-wastes for Bioethanol Production

Beladhadi

¹

,

Shankar

²

,

Jayalakshmi

³

et al. 2021

Waste Biomass Valor

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Purpose: This study focuses to assess the potentiality of the locally isolated Burkholderia sp SMB1. It was aimed for the cocktail of lignocellulolytic enzyme production involved in the depolymerization of the agro-wastes to release sugars for bioethanol production.Methods: The optimization of enzyme cocktail (lignolytic, cellulolytic, hemicellulolytic) production by isolated bacterium utilizing inexpensive substrates like bran and straw of rice was done using response surface methodology. Further this cocktail secreted by the bacterium at optimized conditions was employed for saccharifying untreated agro-wastes for sugars production which were fermented to bioethanol by S. cerevisiae.Results: This strain produced high titer of cellulase (10.8 U/mL), xylanase (76 U/mL), mannanase (14.23 U/mL), pectinase (62.18 U/mL) and laccase (24.25 U/mL) using rice bran at 7 pH and 40 o C using 10% (w/v) of rice bran. The production of cocktail of enzymes was slightly increased by adding CaCO 3 (2mM) in the growth medium. This cocktail was able to hydrolyze untreated agro-wastes to release highest reducing sugars 28.5 g/L using untreated corn husk after 24 h of sacchari cation process. These sugars of corn husk were fermented by S. cerevisiae to produce highest 9.04 gL -1 bioethanol.Conclusion: This paper represents the exploitation of the locally isolated strain for the production of lignocellulolytic enzymes using rice bran and straw for the rst time promising the novelty of the study. This study helps in waste disposal process by valorizing the waste biomass to produce value added products like sugars, bioethanol and hence increases agricultural economy. Statement Of NoveltyThe production of the cocktail of lignolytic, cellulolytic and hemicellulolytic enzymes by the microorganisms has being the interesting topic because of its potentiality of utilizing the lignocellulosic agrowastes for the production of fermentable sugars. These sugars can be directly fermented to bioethanol without any detoxi cation as there may be less or no production of yeast inhibitors during the sacchari cation process through microbial or enzymatic process. Hence, the process becomes economically feasible as the steps required during fermentation (detoxi cation) may be reduced.Moreover, the isolated Burkholderia sp SMB1 becomes novel as it secretes high titer of enzymes after 24 h. This work represents to be the rst study on production of several lignocellulolytic enzymes by Burkholderia sp SMB1 strain utilizing several lignocellulosic agro-wastes. Thus, Burkholderia sp SMB1 strain shows the potentiality for its industrially application in biofuel industries as the cocktail of enzymes were utilized for the sacchari cation of agro-wastes to release fermentable sugars which are fermented to bioethanol by yeast strain.

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Bioprocessing strategies for cost-effective large-scale production of bacterial laccase from Lysinibacillus macroides LSO using bio-waste

Cited by 13 publications

References 30 publications

Biovalorization of raw agro-industrial waste through a bioprocess development platform for boosting alkaline phosphatase production by Lysinibacillus sp. strain APSO

Biovalorization of raw agro-industrial waste through a bioprocess development platform for boosting alkaline phosphatase production by Lysinibacillus sp. strain APSO

Production of Cocktail of Lignolytic, Cellulolytic and Hemicellulolytic Enzymes by the Novel Bacterium Burkholderia Sp SMB1 Utilizing Rice Bran and Straw: Application in the Saccharification of Untreated Agro-Wastes for Bioethanol Production

Production of Cocktail of Lignolytic, Cellulolytic and Hemicellulolytic Enzymes by the Novel Bacterium Burkholderia sp SMB1 Utilizing Rice Bran and Straw: Application in the Saccharification of Untreated Agro-wastes for Bioethanol Production

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