“…Using a direct plating method, the isolates used in this study are isolated from Bunaken Island, North Sulawesi. An educational video on the sampling method of mangrove leaves from several mangrove forests in Indonesia has been shown by Suhendra [15]. Equipment used in the study were orbital shaker, Erlenmeyer 100 ml, 250 ml, and 2000 ml, laminar air flow, microscope and digital camera, personal computer (PC), stirring glass, pro pipette, micropipette, plastic bottle, petri dish, bunsen, aluminum foil, cotton, gauze, ose needle, centrifuge, oven, digital balance sheet, vacuum evaporator, sonicator, beaker glass 100 and 250 ml, distillation device, tube/vial, label paper.…”