Bioprocess Optimization of Microbial Biopolymer Production

Osman, Yehia A.; Elrazak, Ahmed Abd; Khater, Wesam

doi:10.1166/jbmb.2016.1582

Cited by 10 publications

(4 citation statements)

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“…Response surface methodology (RSM), most efficient and commonly used statistical tool (Osman et al 2016) was used to optimize the effects of four most influencing parameters namely, peptone, trace metal solution, yeast extract, (NH 4 ) 2 SO 4 on PHB production. PHB production was taken as the response in designing the experiments.…”

Section: Optimization Of the Significant Media Components By Rsmmentioning

confidence: 99%

Improved fed-batch production of high-purity PHB (poly-3 hydroxy butyrate) by Cupriavidus necator (MTCC 1472) from sucrose-based cheap substrates under response surface-optimized conditions

Dey

Rangarajan

2017

3 Biotech

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Experimental investigations were carried out for (MTCC 1472)-based improved production of poly-3 hydroxy butyrate (PHB) through induced nitrogen limiting fed-batch cultivation strategies. Initially Plackett-Burman design and response surface methodology were implemented to optimize most influencing process parameters. With optimized process parameter values, continuous feeding strategies ware applied in a 5-l fermenter with table sugar concentration of 100 g/l, nitrogen concentration of 0.12 g/l for fed-batch fermentation with varying dilution rates of 0.02 and 0.046 1/h. To get enriched production of PHB, concentration of the sugar was further increased to 150 and 200 g/l in feeding. Maximum concentrations of PHB achieved were 22.35 and 23.07 g/l at those dilution rates when sugar concentration maintains at 200 g/l in feeding. At maximum concentration of PHB (23.07 g/l), productivity of 0.58 g/l h was achieved with maximum PHB accumulation efficiency up to 64% of the dry weight of biomass. High purity of PHB, close to medical grade was achieved after surfactant hypochlorite extraction method, and it was further confirmed by SEM, EDX, and XRD studies.

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Section: Optimization Of the Significant Media Components By Rsmmentioning

confidence: 99%

Improved fed-batch production of high-purity PHB (poly-3 hydroxy butyrate) by Cupriavidus necator (MTCC 1472) from sucrose-based cheap substrates under response surface-optimized conditions

Dey

Rangarajan

2017

3 Biotech

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“…Prodigiosin ( Serratia AM8887 was previously isolated from deep sea core sediments of the Red Sea and was reported to be a potential high PG producer with approximately 100 mg/L when growing on a combination of wastes [7].…”

Section: Discussionmentioning

confidence: 99%

“…For PG production, the isolate was inoculated to the previously generated production medium which consisted of; Sucrose 6 g/l, Glycerol 6 g/l, Fertilizer Waste 12 g/l and NaCl 15 g/l [7]. The organism was cultivated at an agitation speed of 180 rpm and 25°C with a pH of 6.5 for 48hrs in a 250 mL Erlenmeyer flask containing 100 ml of the generated production medium.…”

Section: Strain and Cultivation Conditionsmentioning

confidence: 99%

“…The main target of this study was to optimize and scale up the production of PG from a local isolate of bacterial strain, Serratia AM8887, which has been documented to produce significant amounts of PG [7]. The production was optimized at Bioreactor scale (20L) in terms of pH, temperature and dissolved oxygen levels via applying a Taguchi matrix.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of Bioreactor Cultivation Parameters by Taguchi Orthogonal Array Design for Enhanced Prodigiosin Production

Elmenshawey¹,

Abdelrazak²,

Mowafey³

et al. 2017

Preprint

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One of the major steps toward the industrialization of microbial product(s) is to optimize the cultivation conditions at the large scale bioreactor and successfully control the microbial behavior within large scale production environment. Statistical Design of Experiment was proven to optimize a vast number of microbial processes to achieve robustness and explore possible interactions among the variables. In this research, Taguchi Orthogonal Array was applied to optimize the cultivation condition of a newly isolated Prodigiosin-producing marine bacterial strain, Serratia AM8887, at bioreactor level. Two steps fermentation process was applied; as the productivity was scaled up from shake flask level to a bench top bioreactor (5L) and subsequently to an in-situ sterilization bioreactor system (20L) leading to a yield of 7g/L compared to 100mg/L prior to optimization confirming that; applying Taguchi experimental design is a reliable and good positive option for the optimization of biotechnological processes..

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Microbial Polyhydroxyalkanoates (PHAs): A Review on Biosynthesis, Properties, Fermentation Strategies and Its Prospective Applications for Sustainable Future

2022

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Bioprocess Optimization of Microbial Biopolymer Production

Cited by 10 publications

References 0 publications

Improved fed-batch production of high-purity PHB (poly-3 hydroxy butyrate) by Cupriavidus necator (MTCC 1472) from sucrose-based cheap substrates under response surface-optimized conditions

Improved fed-batch production of high-purity PHB (poly-3 hydroxy butyrate) by Cupriavidus necator (MTCC 1472) from sucrose-based cheap substrates under response surface-optimized conditions

Optimization of Bioreactor Cultivation Parameters by Taguchi Orthogonal Array Design for Enhanced Prodigiosin Production

Microbial Polyhydroxyalkanoates (PHAs): A Review on Biosynthesis, Properties, Fermentation Strategies and Its Prospective Applications for Sustainable Future

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