Bioprocess Engineering Aspects of Biopolymer Production by the Cyanobacterium<i>Spirulina</i>Strain LEB 18

Martins, Roberta Guimarães; Gonçalves, Ígor Severo; Morais, Michele Greque de; Costa, Jorge Alberto Vieira

doi:10.1155/2014/895237

Cited by 33 publications

(7 citation statements)

References 20 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These biocomposites are promising because they present applications in drugs, food and environmental protection . Biopolymers have similar thermoplastic characteristics to petrochemical polymers and have properties such as biodegradability and biocompatibility with cells and tissues (Martins et al, 2014).…”

Section: Characterization Of Biomass Composition Centesimal Compositionmentioning

confidence: 99%

INDUSTRIAL PLANT FOR PRODUCTION OF Spirulina sp. LEB 18

Uebel

Costa

Olson³

et al. 2019

Braz. J. Chem. Eng.

View full text Add to dashboard Cite

Biorefineries based on microalgae produce biofuels and co-products with added value. Microalgae mainly require water, carbon dioxide and sunlight for growth. The bioproducts of the cultivation of these microorganisms can be fully used in a microalgae photobiorefinery. The objective of this work was to study the behavior of physico-chemical variables and kinetic and biological responses in industrial cultivation of Spirulina sp. LEB 18, aiming at the operation of a microalgal photobiorefinery. The maximum specific growth rate (0.133 1/d), the minimum generation time (5.2 d) and maximum productivity (14.9 g/m².d) were obtained in the first 9 d of microalgae growth. The maximum biomass concentration (1.64 g/L) was obtained in 37 d of cultivation. The highest levels of carbohydrates, proteins and lipids in the biomass were 10.6, 57.0 and 11.7%, respectively. The plant monitoring demonstrated that the microalgae produced biomass with high quality for application as biofuels, energy, health and nutrition human.

show abstract

Section: Characterization Of Biomass Composition Centesimal Compositionmentioning

confidence: 99%

INDUSTRIAL PLANT FOR PRODUCTION OF Spirulina sp. LEB 18

Uebel

Costa

Olson³

et al. 2019

Braz. J. Chem. Eng.

View full text Add to dashboard Cite

show abstract

“…Samples were collected aseptically every 24 h to monitor the cell concentration, which was calculated by measuring the optical density at 670 nm in a spectrophotometer (Q7980RM, Quimis, Brazil), with a calibration curve that relate the optical density and dry biomass for each microalgae (Martins et al, 2014). The pH of the cultures was monitored daily with a digital pH meter (pH221, Lutron, Brazil).…”

Section: Monitoring Of the Growth And Responsesmentioning

confidence: 99%

“…The precipitate was then washed with distilled water, centrifuged and washed with acetone while stirring for 10 min. The precipitate was centrifuged to separate the liquid and taken to the oven (Quimis, Q317 M, Brazil) at 35°C for 48 h. The obtained biopolymer was then weighed (Martins et al, 2014).…”

Section: Extraction Of Biopolymer By Digestionmentioning

confidence: 99%

Polyhydroxybutyrate production by Spirulina sp. LEB 18 grown under different nutrient concentrations

Coelho¹,

Silva²,

Terra³

et al. 2015

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

In response to the environmental problems caused by plastics of petrochemical origin, a reduction in the use of these materials and their replacement by biodegradable polymers have been sought. Polyhydroxybutyrate (PHB), a biopolymer of biological origin that belongs to the polyhydroxyalkanoates (PHAs), is similar to polypropylene in terms of its mechanical properties, thermodegradability and melting temperature. Various microorganisms, including cyanobacteria, can synthesize this biopolymer. The objective of this study was to stimulate biopolymer synthesis by Spirulina sp. LEB 18 that was grown under different nutritional conditions. Initially, the growth was conducted with Spirulina sp. LEB 18 without the adaptation of the inoculum. In these assays, the concentrations of the carbon, nitrogen and phosphorus sources were varied. The assay that showed the maximum concentration of biopolymers was reproduced with the adaptation of the inoculum for 45 days. There was an inverse relationship between the cell growth and biopolymer synthesis. The assay that contained 0.25 g L-1 sodium nitrate, 4.4 g L-1 sodium bicarbonate and 0.5 g L-1 potassium phosphate showed the maximum cell concentration (0.6 g L-1) and a low biopolymer accumulation (13.4%). In addition, the assay that contained 0.05 g L-1 sodium nitrate, 8.4 g L-1 sodium bicarbonate and 0.5 g L-1 potassium phosphate produced a high biopolymer concentration (30.7%) and a low cell concentration (0.5 g L-1). The adaptation of the inoculum increased the cell concentration by 7.0% and the biopolymer yield by 20.5%. The biopolymer production was more efficient in assays in which the nitrogen was restricted and had maximum carbon consumption.

show abstract

“…Some cultures are used in wastewater treatment [5] , in fixing carbon dioxide and in biocompound synthesis [6,7] . The biomass of Spirulina has been investigated for its hypocholesterolemic potential [8] , as a source of biofuels [9] and for biopolymer production [10][11][12] . Several genera and species of cyanobacteria, such as Dunaliella tertiolecta [11] , Aulosira fertilissima [12] , Nostoc muscorum [13] , Spirulina subsalsa [14] , Synechocystis sp.…”

Section: Introductionmentioning

confidence: 99%