Bioprocess Developments in the Production of Bioinsecticides by<i>Bacillus Thuringiensis</i>

Rowe, Gerald E.; Margaritis, Argyrios; Dulmage, Howard T.

doi:10.3109/07388558709086986

Cited by 84 publications

(59 citation statements)

References 84 publications

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“…As discussed by Burgerjon & Martouret (1971) many species of larvae did not exhibit any toxaemia from B. thuringiensis due to the fact that the pH in their gut is alkaline. Rowe et al (1987) reported on the annual worldwide distribution of B. thuringiensis amount to be 2.3 × 10 6 kg. Worldwide sales of B. thuringiensis were $90 million, representing about 2% of the total global insecticide market.…”

Section: Resultsmentioning

confidence: 99%

Insecticidal effects of some biological agents on Agelastica alni (Coleoptera: Chrysomelidae)

Sezen

Demirbağ

2006

Biologia

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Abstract:The alder leaf beetle (Agelastica alni L., Coleoptera: Chrysomelidae) causes approximately 10% of total economic damage to hazelnut product per year in Turkey. A. alni larvae are susceptible to several pathogens indigenous to the area in which these insects occur in Turkey. In the present study, in order to find a more effective and safer biological control agent against this common pest, we evaluated the various biological agents' insecticidal activity during the four hazelnut seasons from 2002 to 2005 on the larvae of the alder leaf beetle collected from the vicinity of Trabzon, Turkey. The tested agents are 25 insect-originating bacteria, 2 bacterial toxins and 1 viral preparation. The results showed that the highest insecticidal activity was obtained by bacterial isolates at 1.8 × 10 9 bacteria/mL dose, within ten days on the larvae of A. alni. These are 90% for Bacillus thuringiensis biovar tenebrionis (4AA1), Bacillus sphaericus (Ar4, isolated from Anoplus roboris L., Col.: Curculionidae), and Bacillus thuringiensis (Mm2, isolated from Melolontha melolontha L., Col.: Scarabaeidae). Our results indicate that these isolates may be valuable as biological control agent.

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Section: Resultsmentioning

confidence: 99%

Insecticidal effects of some biological agents on Agelastica alni (Coleoptera: Chrysomelidae)

Sezen

Demirbağ

2006

Biologia

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“…It produces different insecticidal toxic proteins in parasporal crystals during the stationary phase of its growth cycle (Rowe et al 1987). The genes coding for cry proteins are mostly carried on plasmids ranging from 3-4 to 150 Mda (Gonzales & Carlton 1980;Aronson 2002).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of Bacillus thuringiensis strains from different grain habitats in Turkey

Apaydin

Yenidünya

Harsa

et al. 2005

World J Microbiol Biotechnol

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SummaryBacillus thuringiensis (Bt) is a gram-positive, spore-forming bacterium and it produces insecticidal crystal (cry) proteins during sporulation. Because the genetic diversity and toxic potential of Bt strains differ from region to region, strains have been collected and characterized all over the world. The aim of this study is to isolate Bt strains in grain-related habitats in Turkey and to characterize them on the basis of crystal morphology, cry gene content, and chromosomal and plasmid DNA profiles. Four approaches were taken: analysis with phase contrast (PC) microscopy, polymerase chain reaction (PCR), pulsed field gel electrophoresis (PFGE) and plasmid isolation. Ninety-six samples were collected from Central Anatolia and the Aegean region. Bt was isolated from 61 of 96 samples (63.5%) and 500 Bt-like colonies were obtained. One hundred and sixty three of the colonies were identified as Bt based on cry protein formation using PC microscopy. Among the examined colonies, the overall proportion identified (as Bt index) was 0.33. We found that 103 isolates were positive for the five different cry genes (cry1, cry2, cry3, cry4 and cry9) examined with PCR. In addition, plasmid profiling of 37 cry gene-positive isolates indicated that the 15 kb plasmid band was present in all isolates; however, 11 of 37 isolates had more than one plasmid band at different sizes. Finally, chromosomal DNA profiling by PFGE gave rise to different DNA patterns for isolates containing the same cry gene which suggests a high level of diversity among the Bt strains isolated.

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“…Bacillus thuringiensis (Berliner) (Bacillales: Bacillaceae) is a Gram positive, spore-forming bacterium that produces parasporal crystal inclusions during sporulation (Rowe et al, 1987;. These parasporal crystals, also known as delta-endotoxins or insecticidal crystal proteins (ICPs), are mostly composed of one or more crystal (Cry) proteins and cytolytic proteins (Cyt) (Crickmore et al, 1998;Schnepf et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Bioactivities of cry gene positive Bacillus thuringiensis (Berliner) (Bacillales: Bacillaceae) strains on Ephestia kuehniella Zeller, 1879 and Plodia interpunctella (Hübner, 1813) (Lepidoptera: Pyralidae)

Alper

Güneş

Çöl

et al. 2016

Turkish Journal of Entomology

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Bioprocess Developments in the Production of Bioinsecticides byBacillus Thuringiensis

Cited by 84 publications

References 84 publications

Insecticidal effects of some biological agents on Agelastica alni (Coleoptera: Chrysomelidae)

Insecticidal effects of some biological agents on Agelastica alni (Coleoptera: Chrysomelidae)

Isolation and characterization of Bacillus thuringiensis strains from different grain habitats in Turkey

Bioactivities of cry gene positive Bacillus thuringiensis (Berliner) (Bacillales: Bacillaceae) strains on Ephestia kuehniella Zeller, 1879 and Plodia interpunctella (Hübner, 1813) (Lepidoptera: Pyralidae)

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