Bioprocess Development for the Production of an Antifungal Molecule by Bacillus licheniformis BC98

PATEL, VAISHALI J.; Tendulkar, Sachin R; Chattoo, Bharat B.

doi:10.1263/jbb.98.231

Cited by 9 publications

(12 citation statements)

References 14 publications

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“…Assays for isolation of antifungal antagonists was devised based on morphological changes induced in the germinating spores of M. grisea , leading to a number of bacterial antagonists, and one of these highly effective against development of appressoria in this fungus, was selected for further studies. The conditions for maximum yield of antifungal peptide had been optimized earlier (Patel et al. 2004).…”

Section: Resultsmentioning

confidence: 99%

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Isolation, purification and characterization of an antifungal molecule produced by Bacillus licheniformis BC98, and its effect on phytopathogen Magnaporthe grisea

Tendulkar

Saikumari

Patel

et al. 2007

Journal of Applied Microbiology

152

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Aims: Isolation of bacterial antagonist for use in the biological control of phytopathogenic fungi like rice blast fungus, Magnaporthe grisea, and to further purify and characterize the antifungal molecule produced by the antagonist. Methods and Results: Bacterial antagonist exhibiting highest antifungal activity against the rice blast fungus M. grisea was isolated from soil and identified as Bacillus licheniformis BC98. Besides M. grisea, the isolate also inhibited the growth of other phytopathogens such as Curvularia lunata and Rhizoctonia bataticola. Biologically active fractions were isolated from the culture filtrate and further fractionated by reverse‐phase high‐performance liquid chromatography (HPLC) enabling detailed structural characterization of a component of molecular mass 1035 Da. The active peptide was identified as surfactin after 500 MHz 1H NMR analysis. Microscopic analysis of the effect of the antagonist on M. grisea revealed bulbous hyphae showing patchy and vacuolated cytoplasm when observed under the electron microscope. Conclusions: The antagonistic lipopeptide secreted by B. licheniformis BC98 and identified as surfactin, induced morphological changes in M. grisea, inhibiting its further growth, and thus exhibiting fungicidal activity. Significance and Impact of the Study: The antagonist inhibits germination of M. grisea, a potent rice phytopathogen, and therefore appears to be a potential candidate for control of rice blast disease.

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Section: Resultsmentioning

confidence: 99%

“…Media and process parameters for better production and purification of antifungal molecule from B. licheniformis were performed as reported (Patel et al. 2004).…”

Section: Methodsmentioning

confidence: 99%

Isolation, purification and characterization of an antifungal molecule produced by Bacillus licheniformis BC98, and its effect on phytopathogen Magnaporthe grisea

Tendulkar

Saikumari

Patel

et al. 2007

Journal of Applied Microbiology

152

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“…1994) and fungi (Lebbadi et al. 1994; Patel, Tendulkar & Chattoo 2004). Thus, microorganisms from nest‐lining feathers and antimicrobial chemicals producers might be transferred or migrate to the eggshell preventing colonization of pathogenic bacteria of embryos by directly coming in contact with incubated eggs.…”

Section: Introductionmentioning

confidence: 99%

Number and colour composition of nest lining feathers predict eggshell bacterial community in barn swallow nests: an experimental study

et al. 2010

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Summary 1. The use of feathers as nest lining material has traditionally been explained by the thermoregulatory properties of feathers. Feather nest lining could additionally affect nest detectability by predators, or play a role in a sexually selected context. Furthermore, feather nest lining harbours microorganisms that may influence environmental conditions where eggs and nestlings develop. 2. Microorganisms growing on nest lining feathers could affect the bacterial load of eggshells because they occupy space and/or produce antimicrobial substances against other bacteria, including egg pathogens. Feathers of different colours are known to differ in their bacterial community (i.e. feather degrading bacteria) and, thus, colour composition of nest lining feather could also affect the bacterial environment of avian nests. 3. Here we tested this hypothesis in the barn shallow (Hirundo rustica) by exploring the relationship between eggshell bacterial loads and number of feathers, and the effect of experimentally modified colour composition of nest lining feathers on eggshell bacterial load. 4. In agreement with the hypothesis we found that, before treatment, the number of nest lining feathers (mainly that of unpigmented‐white colour) predicted eggshell bacterial load, and that, at the end of the incubation period, eggshells of experimental nests with white feathers had a lower bacterial density than those in experimental nests with black feathers. 5. We failed to detect a relationship between bacterial load and hatching success. However, since evidence of that relationship exists for other species, these results would explain the previously detected experimental effect of colour composition of nest lining feathers on hatching success of swallows. 6. Nest design in general, and the use of nest‐lining white feathers in particular, may therefore have important consequences for reproductive success of birds. The reduced eggshell bacterial loads of experimental white nests would explain preferences by barn swallows for feathers of white colour.

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“…Many strains of bacteria degrade feathers with Bacillus licheniformis being particularly important (Williams et al 1990;Burtt & Ichida 1999). Bacillus licheniformis degrades feathers (Williams et al 1990;Burtt & Ichida 1999), but also produces antimicrobial substances (Simlot, Specht & Pfaender 1972;Haavik 1974) that are active against bacteria of the genera Bacillus, Corynebacterium, Enterococcus, Micobacterium, amoebae ) and fungi Patel, Tendulkar & Chattoo 2004). Thus, because of the antimicrobial properties of such substances B. licheniformis may impede the establishment of other more virulent bacteria.…”

Section: Introductionmentioning

confidence: 99%

Feather micro‐organisms and uropygial antimicrobial defences in a colonial passerine bird

2009

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Summary 1.The relationship between the composition of communities of micro-organisms and their hosts remains poorly understood. We conducted extensive field studies of feather-degrading bacteria, other cultivable bacteria, and fungi on the plumage of a migratory bird, the barn swallow Hirundo rustica Linnaeus, to understand the association between micro-organisms, host sociality and host antimicrobial defences, as reflected by the size of the uropygial gland. 2. The abundance of feather-degrading bacteria, but not other cultivable bacteria or fungi, decreased with increasing size of the uropygial gland. 3. Females had more feather-degrading bacteria than males. 4. Barn swallows living in larger colonies had more feather-degrading bacteria than less social conspecifics. 5. These findings suggest that the uropygial gland plays a specific role in regulating the abundance of feather-degrading bacteria that furthermore depends on the social environment of the host.

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Bioprocess Development for the Production of an Antifungal Molecule by Bacillus licheniformis BC98

Cited by 9 publications

References 14 publications

Isolation, purification and characterization of an antifungal molecule produced by Bacillus licheniformis BC98, and its effect on phytopathogen Magnaporthe grisea

Isolation, purification and characterization of an antifungal molecule produced by Bacillus licheniformis BC98, and its effect on phytopathogen Magnaporthe grisea

Number and colour composition of nest lining feathers predict eggshell bacterial community in barn swallow nests: an experimental study

Feather micro‐organisms and uropygial antimicrobial defences in a colonial passerine bird

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