Bioprinting of Collagen: Considerations, Potentials, and Applications

Lee, Jia Min; Suen, Sean Kang Qiang; Ng, Wei Long; Cheung, William K.; Yeong, Wai Yee

doi:10.1002/mabi.202000280

Cited by 75 publications

(50 citation statements)

References 273 publications

(358 reference statements)

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“…Generally, ECM proteins enhance cell attachment and proliferation by interacting via integrins or other cell adhesion molecules presented on a cell’s membrane [ 11 , 12 , 13 , 14 , 15 ]. This means that cell receptors recognize the sequence of polypeptide chains in collagen molecules [ 16 ]. Another approach is to coat an ECM-based component such as collagen or fibronectin by physical adsorption that occurs as the result of weak electrostatic and van der Waals interactions between the ECM-based material and PDMS.…”

Section: Introductionmentioning

confidence: 99%

Surface Modification of PDMS-Based Microfluidic Devices with Collagen Using Polydopamine as a Spacer to Enhance Primary Human Bronchial Epithelial Cell Adhesion

et al. 2021

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Polydimethylsiloxane (PDMS) is a silicone-based synthetic material used in various biomedical applications due to its properties, including transparency, flexibility, permeability to gases, and ease of use. Though PDMS facilitates and assists the fabrication of complicated geometries at micro- and nano-scales, it does not optimally interact with cells for adherence and proliferation. Various strategies have been proposed to render PDMS to enhance cell attachment. The majority of these surface modification techniques have been offered for a static cell culture system. However, dynamic cell culture systems such as organ-on-a-chip devices are demanding platforms that recapitulate a living tissue microenvironment’s complexity. In organ-on-a-chip platforms, PDMS surfaces are usually coated by extracellular matrix (ECM) proteins, which occur as a result of a physical and weak bonding between PDMS and ECM proteins, and this binding can be degraded when it is exposed to shear stresses. This work reports static and dynamic coating methods to covalently bind collagen within a PDMS-based microfluidic device using polydopamine (PDA). These coating methods were evaluated using water contact angle measurement and atomic force microscopy (AFM) to optimize coating conditions. The biocompatibility of collagen-coated PDMS devices was assessed by culturing primary human bronchial epithelial cells (HBECs) in microfluidic devices. It was shown that both PDA coating methods could be used to bind collagen, thereby improving cell adhesion (approximately three times higher) without showing any discernible difference in cell attachment between these two methods. These results suggested that such a surface modification can help coat extracellular matrix protein onto PDMS-based microfluidic devices.

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Section: Introductionmentioning

confidence: 99%

Surface Modification of PDMS-Based Microfluidic Devices with Collagen Using Polydopamine as a Spacer to Enhance Primary Human Bronchial Epithelial Cell Adhesion

et al. 2021

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“…In addition, collagen fibrillogenesis and its physical crosslinking becomes effective when the collagen is neutralized and at 37 °C by forming thicker and stronger fibrous structure. [29,30] To determine whether HT-ink was appropriate for EBB, we evaluated its rheo-logical properties. As IOB of the HT-ink was performed without cell encapsulation, the rheology study was performed with the acellular HT-ink.…”

Section: Resultsmentioning

confidence: 99%

Intra‐Operative Bioprinting of Hard, Soft, and Hard/Soft Composite Tissues for Craniomaxillofacial Reconstruction

Moncal

Gudapati

Godzik

et al. 2021

Adv Funct Materials

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Reconstruction of complex craniomaxillofacial (CMF) defects is challenging dueto the highly organized layering of multiple tissue types. Such compartmentalization necessitates the precise and effective use of cells and other biologics to recapitulate the native tissue anatomy. In this study, intra-operative bioprinting (IOB) of different CMF tissues, including bone, skin, and composite (hard/soft) tissues, is demonstrated directly on rats in a surgical setting. A novel extrudable osteogenic hard tissue ink is introduced, which induced substantial bone regeneration, with ≈80% bone coverage area of calvarial defects in 6 weeks. Using droplet-based bioprinting, the soft tissue ink accelerated the reconstruction of full-thickness skin defects and facilitated up to 60% wound closure in 6 days. Most importantly, the use of a hybrid IOB approach is unveiled to reconstitute hard/soft composite tissues in a stratified arrangement with controlled spatial bioink deposition conforming the shape of a new composite defect model, which resulted in ≈80% skin wound closure in 10 days and 50% bone coverage area at Week 6. The presented approach will be absolutely unique in the clinical realm of CMF defects and will have a significant impact on translating bioprinting technologies into the clinic in the future.

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“…All methods are considered as additive biomanufacturing technologies when complex shapes with internal structures without molds or shaping tools are produced [ 84 ]. In the first two groups, the material is deposited through the nozzle, when the final structure is created in the resolution which is given: (1) flow rate ratio between the extrudate and printing platform, (2) the nozzle size (3) height of the nozzle above the platform [ 85 ]. The own extrusion is driven pneumatically or mechanically by a piston or by a screw [ 86 ].…”

Section: Resultsmentioning

confidence: 99%

Collagen Bioinks for Bioprinting: A Systematic Review of Hydrogel Properties, Bioprinting Parameters, Protocols, and Bioprinted Structure Characteristics

et al. 2021

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Bioprinting is a modern tool suitable for creating cell scaffolds and tissue or organ carriers from polymers that mimic tissue properties and create a natural environment for cell development. A wide range of polymers, both natural and synthetic, are used, including extracellular matrix and collagen-based polymers. Bioprinting technologies, based on syringe deposition or laser technologies, are optimal tools for creating precise constructs precisely from the combination of collagen hydrogel and cells. This review describes the different stages of bioprinting, from the extraction of collagen hydrogels and bioink preparation, over the parameters of the printing itself, to the final testing of the constructs. This study mainly focuses on the use of physically crosslinked high-concentrated collagen hydrogels, which represents the optimal way to create a biocompatible 3D construct with sufficient stiffness. The cell viability in these gels is mainly influenced by the composition of the bioink and the parameters of the bioprinting process itself (temperature, pressure, cell density, etc.). In addition, a detailed table is included that lists the bioprinting parameters and composition of custom bioinks from current studies focusing on printing collagen gels without the addition of other polymers. Last but not least, our work also tries to refute the often-mentioned fact that highly concentrated collagen hydrogel is not suitable for 3D bioprinting and cell growth and development.

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Bioprinting of Collagen: Considerations, Potentials, and Applications

Cited by 75 publications

References 273 publications

Surface Modification of PDMS-Based Microfluidic Devices with Collagen Using Polydopamine as a Spacer to Enhance Primary Human Bronchial Epithelial Cell Adhesion

Surface Modification of PDMS-Based Microfluidic Devices with Collagen Using Polydopamine as a Spacer to Enhance Primary Human Bronchial Epithelial Cell Adhesion

Intra‐Operative Bioprinting of Hard, Soft, and Hard/Soft Composite Tissues for Craniomaxillofacial Reconstruction

Collagen Bioinks for Bioprinting: A Systematic Review of Hydrogel Properties, Bioprinting Parameters, Protocols, and Bioprinted Structure Characteristics

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