Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization

Thayer, Patrick; Orrhult, Linnea Stridh; Martínez, Héctor

doi:10.3791/56372

Cited by 15 publications

(16 citation statements)

References 32 publications

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“…The biomaterial and mixing technique described here could also be beneficially implemented in combination with automation techniques like 3D bioprinting or injection molding. In the extrusion printing of high-collagen dense constructs, cells need to be mixed thoroughly (e.g., 40 aspiration cycles) with the scaffold material used in advance to create the bioink [29,30]. In our mixing technique, the biomaterial is only mixed gently with the cells at the moment of extrusion.…”

Section: Discussionmentioning

confidence: 99%

A simplified fabrication technique for cellularized high-collagen dermal equivalents

et al. 2019

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Human autologous bioengineered skin has been successfully developed and used to treat skin injuries in a growing number of cases. In current clinical studies, the biomaterial used is fabricated via plastic compression of collagen hydrogel to increase the density and stability of the tissue. To further facilitate clinical adoption of bioengineered skin, the fabrication technique needs to be improved in terms of standardization and automation. Here, we present a one-step mixing technique using highly concentrated collagen and human fibroblasts to simplify fabrication of stable dermal equivalents. As controls, we prepared cellularized dermal equivalents with three varying collagen compositions. We found that the dermal equivalents produced using the simplified mixing technique were stable and pliable, showed viable fibroblast distribution throughout the tissue, and were comparable to highly concentrated manually produced collagen gels. Because no subsequent plastic compression of collagen is required in the simplified mixing technique, the fabrication steps and production time for dermal equivalents are consistently reduced. The present study provides a basis for further investigations to optimize the technique, which has significant promise in enabling efficient clinical production of bioengineered skin in the future.

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Section: Discussionmentioning

confidence: 99%

A simplified fabrication technique for cellularized high-collagen dermal equivalents

et al. 2019

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“…Needle-based bioprinting techniques are more popular than other methods due to accessibility and ease of using Luer-lock syringe tips [35,36]. However, needle tips are prone to clogging, especially at diameters under 150 μm [37].…”

Section: 3d Bioprinting Techniquesmentioning

confidence: 99%

iPSC Bioprinting: Where are We at?

2019

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Here, we present a concise review of current 3D bioprinting technologies applied to induced pluripotent stem cells (iPSC). iPSC have recently received a great deal of attention from the scientific and clinical communities for their unique properties, which include abundant adult cell sources, ability to indefinitely self-renew and differentiate into any tissue of the body. Bioprinting of iPSC and iPSC derived cells combined with natural or synthetic biomaterials to fabricate tissue mimicked constructs, has emerged as a technology that might revolutionize regenerative medicine and patient-specific treatment. This review covers the advantages and disadvantages of bioprinting techniques, influence of bioprinting parameters and printing condition on cell viability, and commonly used iPSC sources, and bioinks. A clear distinction is made for bioprinting techniques used for iPSC at their undifferentiated stage or when used as adult stem cells or terminally differentiated cells. This review presents state of the art data obtained from major searching engines, including Pubmed/MEDLINE, Google Scholar, and Scopus, concerning iPSC generation, undifferentiated iPSC, iPSC bioprinting, bioprinting techniques, cartilage, bone, heart, neural tissue, skin, and hepatic tissue cells derived from iPSC.

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“…Several companies are currently involved in producing 3D bioprinters and commercializing the printing of large tissues. These include CellInk and Organovo [184][185][186][187][188][189][190]. Though faced with many challenges including scaling up of the processes, regenerative medicine technologies are expanding and are likely to impact on patients' treatment in future.…”

Section: Manufacturing and Regulatory Process Considerationsmentioning

confidence: 99%

Trends in Decellularized Extracellular Matrix Bioinks for 3D Printing: Inspiring a new Kind of Medicine

Dzobo¹,

Motaung²,

Adesida³

2019

Preprint

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The promise of regenerative medicine and tissue engineering is founded on the ability to regenerate diseased or damaged tissues and organs into functional tissues and organs or the creation of new tissues and organs altogether. In theory, all damaged and diseased tissues and organs can be regenerated or created using different configurations and combinations of extracellular matrix, cells and inductive biomolecules. Currently, regenerative medicine and tissue engineering can allow the improvement of patients’ quality of life through availing novel treatment options. Tissues and organs have a specific ECM, with specific proteins and factors released by cells residing within the local microenvironment. The coupling of regenerative medicine and tissue engineering field with 3D printing is revolutionizing the treatment of patients in a huge way. 3D bioprinting allows the proper placement of cells and ECMs, allowing the recapitulation of native microenvironments of tissues and organs. 3D bioprinting utilizes different bioinks made up of different formulations of ECM/biomaterials, biomolecules and even cells. The choice of the bioink used during 3D bioprinting is very important as properties such as printability, compatibility and physical strength influence the final construct printed. The extracellular matrix (ECM) provides both physical and mechanical microenvironment needed by cells to survive and proliferate. Decellularized ECM bioink contains biochemical cues from the original native ECM and also the right proportions of ECM proteins. Different techniques and characterization methods are used to derive bioinks from several tissues and organs and to evaluate their quality. This review discusses the uses of decellularized ECM bioinks and argues that they represent the most biomimetic bioinks available. In addition, we briefly discuss some polymer-based bioinks utilized in 3D bioprinting.

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Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization

Cited by 15 publications

References 32 publications

A simplified fabrication technique for cellularized high-collagen dermal equivalents

A simplified fabrication technique for cellularized high-collagen dermal equivalents

iPSC Bioprinting: Where are We at?

Trends in Decellularized Extracellular Matrix Bioinks for 3D Printing: Inspiring a new Kind of Medicine

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