Bioprinting in Vascularization Strategies

Jafarkhani, Mahboubeh; Salehi, Zeinab; Aidun, Amir; Shokrgozar, Mohammad Ali

doi:10.29252/ibj.23.1.9

Cited by 40 publications

(22 citation statements)

References 60 publications

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“…Layer by layer printed scaffolds showed higher cell proliferation and growth when compared to conventional non-printed 3D scaffold cultures 35 . Facilitated media diffusion, easy waste removal and relatively homogenous drug diffusion can be achieved by using 3D bioprinting of cell laden scaffolds with designed pore size and good rheological properties when compared to non-printed 3D cultures [36][37][38][39] . Bioprinting can also prevent core necrosis which is the common problem of non-printed polymer-based 3D models 40 .…”

Section: Discussionmentioning

confidence: 99%

Polysaccharide hydrogel based 3D printed tumor models for chemotherapeutic drug screening

Gebeyehu

Surapaneni

Huang³

et al. 2021

Sci Rep

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A series of stable and ready-to-use bioinks have been developed based on the xeno-free and tunable hydrogel (VitroGel) system. Cell laden scaffold fabrication with optimized polysaccharide-based inks demonstrated that Ink H4 and RGD modified Ink H4-RGD had excellent rheological properties. Both bioinks were printable with 25–40 kPa extrusion pressure, showed 90% cell viability, shear-thinning and rapid shear recovery properties making them feasible for extrusion bioprinting without UV curing or temperature adjustment. Ink H4-RGD showed printability between 20 and 37 °C and the scaffolds remained stable for 15 days at temperature of 37 °C. 3D printed non-small-cell lung cancer (NSCLC) patient derived xenograft cells (PDCs) showed rapid spheroid growth of size around 500 µm in diameter and tumor microenvironment formation within 7 days. IC50 values demonstrated higher resistance of 3D spheroids to docetaxel (DTX), doxorubicin (DOX) and erlotinib compared to 2D monolayers of NSCLC-PDX, wild type triple negative breast cancer (MDA-MB-231 WT) and lung adenocarcinoma (HCC-827) cells. Results of flow property, shape fidelity, scaffold stability and biocompatibility of H4-RGD suggest that this hydrogel could be considered for 3D cell bioprinting and also for in-vitro tumor microenvironment development for high throughput screening of various anti-cancer drugs.

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Section: Discussionmentioning

confidence: 99%

Polysaccharide hydrogel based 3D printed tumor models for chemotherapeutic drug screening

Gebeyehu

Surapaneni

Huang³

et al. 2021

Sci Rep

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“…When examining the printed tissue's physiological needs, we see that the limit of diffusion for O 2 in tissue is generally accepted to be limited to 100-200 μm (Krogh, 1919;Carmeliet and Jain, 2000;Jain et al, 2005). This printed vascular network is essential for the delivery of nutrients and oxygen along with the removal of waste products and CO 2 (Jafarkhani et al, 2019). The vasculature in the human body ranges from very large (mm size) to microscopic (μm; Figure 2; Schöneberg et al, 2018;Tomasina et al, 2019).…”

Section: Preparation Of Vascular Conduitsmentioning

confidence: 99%

3D Bioprinting of Vascularized Tissues for in vitro and in vivo Applications

Chen

Toksoy

Davis

et al. 2021

Front. Bioeng. Biotechnol.

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With a limited supply of organ donors and available organs for transplantation, the aim of tissue engineering with three-dimensional (3D) bioprinting technology is to construct fully functional and viable tissue and organ replacements for various clinical applications. 3D bioprinting allows for the customization of complex tissue architecture with numerous combinations of materials and printing methods to build different tissue types, and eventually fully functional replacement organs. The main challenge of maintaining 3D printed tissue viability is the inclusion of complex vascular networks for nutrient transport and waste disposal. Rapid development and discoveries in recent years have taken huge strides toward perfecting the incorporation of vascular networks in 3D printed tissue and organs. In this review, we will discuss the latest advancements in fabricating vascularized tissue and organs including novel strategies and materials, and their applications. Our discussion will begin with the exploration of printing vasculature, progress through the current statuses of bioprinting tissue/organoids from bone to muscles to organs, and conclude with relevant applications for in vitro models and drug testing. We will also explore and discuss the current limitations of vascularized tissue engineering and some of the promising future directions this technology may bring.

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“…Printing is also an important step. (d) Postprocessing of printed objects, it is worth mentioning that in 3D printing of anatomical models and tissues and organs, doctors can use medical imaging data obtained from CT and MRI to construct STL without drawing 3D models themselves (Bucking et al, 2017; Datta et al, 2018; Jafarkhani, Salehi, Aidun, & Shokrgozar, 2019).…”

Section: Overview Of 3d Printing Technologiesmentioning

confidence: 99%

Three‐dimensional printing: The potential technology widely used in medical fields

Fan

Zhu

2020

J Biomedical Materials Res

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Three‐dimensional (3D) printing technology is one of the hottest research fields nowadays, which has influenced the treatment methods in the medical industry. In order to explore the progress of 3D printing technology in medical applications, the authors conducted English retrieval with the keywords “three‐dimensionalprinting,” “bioprinting,” and “3D printing” in PubMed, and extracted information related to this exploration. This review firstly introduces the 3D printing materials, types of technology, and printing procedures in medical fields, then elaborates the current applications of 3D printing in medical devices, in vitro anatomical models, organ printing, implants, tissue engineering scaffolds, and the leap from 3D medical printing to four‐dimensional (4D) medical printing, finally put forward the shortage of the medical 3D/4D printing and possible solutions of them.

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Bioprinting in Vascularization Strategies

Cited by 40 publications

References 60 publications

Polysaccharide hydrogel based 3D printed tumor models for chemotherapeutic drug screening

Polysaccharide hydrogel based 3D printed tumor models for chemotherapeutic drug screening

3D Bioprinting of Vascularized Tissues for in vitro and in vivo Applications

Three‐dimensional printing: The potential technology widely used in medical fields

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