Bioprinting-Based High-Throughput Fabrication of Three-Dimensional MCF-7 Human Breast Cancer Cellular Spheroids

Ling, Kai; Huang, Guoman; Liu, Juncong; Zhang, Xiaohui; Ma, Yufei; Lu, Tianjian; Xu, Feng

doi:10.15302/j-eng-2015062

Cited by 98 publications

(62 citation statements)

References 33 publications

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“… 122 , 123 Additionally, BC cells cultured in a custom-built bioprinting platform demonstrated more in vivo - lik e and uniform cellular spheroids in situ on a hydrogel substrate, representing a controllable and high-throughput approach for modelling PTM. 124 …”

Section: Three-dimensional Approaches To Model Cancer Bone Metastasismentioning

confidence: 99%

Engineering 3D approaches to model the dynamic microenvironments of cancer bone metastasis

Qiao

Tang

2018

Bone Res

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Cancer metastasis to bone is a three-dimensional (3D), multistep, dynamic process that requires the sequential involvement of three microenvironments, namely, the primary tumour microenvironment, the circulation microenvironment and the bone microenvironment. Engineered 3D approaches allow for a vivid recapitulation of in vivo cancerous microenvironments in vitro, in which the biological behaviours of cancer cells can be assessed under different metastatic conditions. Therefore, modelling bone metastasis microenvironments with 3D cultures is imperative for advancing cancer research and anti-cancer treatment strategies. In this review, multicellular tumour spheroids and bioreactors, tissue engineering constructs and scaffolds, microfluidic systems and 3D bioprinting technology are discussed to explore the progression of the 3D engineering approaches used to model the three microenvironments of bone metastasis. We aim to provide new insights into cancer biology and advance the translation of new therapies for bone metastasis.

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Section: Three-dimensional Approaches To Model Cancer Bone Metastasismentioning

confidence: 99%

Engineering 3D approaches to model the dynamic microenvironments of cancer bone metastasis

Qiao

Tang

2018

Bone Res

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“…High cost for the microfabrication; HTS options limited [80,81] 3D bioprinting 3D bioprinting fibers embedded in hydrogels to recreate microvessels and study cancer-related angiogenesis [82]; Proliferation and invasion ability [83]; Modeling tumor microenvironment [84]; Migration of osteotropic cells during bone metastasis [85];…”

Section: Required Expertise;mentioning

confidence: 99%

Trends in Bone Metastasis Modeling

Laranga

Duchi

Ibrahim

et al. 2020

Cancers

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Bone is one of the most common sites for cancer metastasis. Bone tissue is composed by different kinds of cells that coexist in a coordinated balance. Due to the complexity of bone, it is impossible to capture the intricate interactions between cells under either physiological or pathological conditions. Hence, a variety of in vivo and in vitro approaches have been developed. Various models of tumor–bone diseases are routinely used to provide valuable information on the relationship between metastatic cancer cells and the bone tissue. Ideally, when modeling the metastasis of human cancers to bone, models would replicate the intra-tumor heterogeneity, as well as the genetic and phenotypic changes that occur with human cancers; such models would be scalable and reproducible to allow high-throughput investigation. Despite the continuous progress, there is still a lack of solid, amenable, and affordable models that are able to fully recapitulate the biological processes happening in vivo, permitting a correct interpretation of results. In the last decades, researchers have demonstrated that three-dimensional (3D) methods could be an innovative approach that lies between bi-dimensional (2D) models and animal models. Scientific evidence supports that the tumor microenvironment can be better reproduced in a 3D system than a 2D cell culture, and the 3D systems can be scaled up for drug screening in the same way as the 2D systems thanks to the current technologies developed. However, 3D models cannot completely recapitulate the inter- and intra-tumor heterogeneity found in patients. In contrast, ex vivo cultures of fragments of bone preserve key cell–cell and cell–matrix interactions and allow the study of bone cells in their natural 3D environment. Moreover, ex vivo bone organ cultures could be a better model to resemble the human pathogenic metastasis condition and useful tools to predict in vivo response to therapies. The aim of our review is to provide an overview of the current trends in bone metastasis modeling. By showing the existing in vitro and ex vivo systems, we aspire to contribute to broaden the knowledge on bone metastasis models and make these tools more appealing for further translational studies.

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“…Ling et al investigated tumor spheroid formation using MCF-7s encapsulated in sacrificial gelatin arrays. Later, the arrays were used to fabricate PEG-dimethacrylate concave wells (Ling et al, 2015 ). The combination of gelatin and PEG allowed for a single tumor spheroid formation step, eliminating the need for an additional seeding step.…”

Section: D Bioprintingmentioning

confidence: 99%

Engineering Breast Cancer Microenvironments and 3D Bioprinting

Belgodere

King

Bursavich

et al. 2018

Front. Bioeng. Biotechnol.

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The extracellular matrix (ECM) is a critical cue to direct tumorigenesis and metastasis. Although two-dimensional (2D) culture models have been widely employed to understand breast cancer microenvironments over the past several decades, the 2D models still exhibit limited success. Overwhelming evidence supports that three dimensional (3D), physiologically relevant culture models are required to better understand cancer progression and develop more effective treatment. Such platforms should include cancer-specific architectures, relevant physicochemical signals, stromal–cancer cell interactions, immune components, vascular components, and cell-ECM interactions found in patient tumors. This review briefly summarizes how cancer microenvironments (stromal component, cell-ECM interactions, and molecular modulators) are defined and what emerging technologies (perfusable scaffold, tumor stiffness, supporting cells within tumors and complex patterning) can be utilized to better mimic native-like breast cancer microenvironments. Furthermore, this review emphasizes biophysical properties that differ between primary tumor ECM and tissue sites of metastatic lesions with a focus on matrix modulation of cancer stem cells, providing a rationale for investigation of underexplored ECM proteins that could alter patient prognosis. To engineer breast cancer microenvironments, we categorized technologies into two groups: (1) biochemical factors modulating breast cancer cell-ECM interactions and (2) 3D bioprinting methods and its applications to model breast cancer microenvironments. Biochemical factors include matrix-associated proteins, soluble factors, ECMs, and synthetic biomaterials. For the application of 3D bioprinting, we discuss the transition of 2D patterning to 3D scaffolding with various bioprinting technologies to implement biophysical cues to model breast cancer microenvironments.

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Bioprinting-Based High-Throughput Fabrication of Three-Dimensional MCF-7 Human Breast Cancer Cellular Spheroids

Cited by 98 publications

References 33 publications

Engineering 3D approaches to model the dynamic microenvironments of cancer bone metastasis

Engineering 3D approaches to model the dynamic microenvironments of cancer bone metastasis

Trends in Bone Metastasis Modeling

Engineering Breast Cancer Microenvironments and 3D Bioprinting

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