Bioprinting-associated pulsatile hydrostatic pressure elicits a mild proinflammatory response in epi- and endothelial cells

Nasehi, Ramin; Schieren, Jana; Grannemann, Caroline; Palkowitz, Alena L.; Babendreyer, Aaron; Schwarz, Nicole; Aveic, Sanja; Ludwig, Andreas; Leube, Rudolf E.; Fischer, Horst

doi:10.1016/j.bioadv.2023.213329

Cited by 1 publication

(1 citation statement)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 172 ] In addition, bioprinting‐associated pulsatile hydrostatic pressure can elicit a proinflammatory response in cells. [ 173 ] Therefore, new printing technologies are being developed to enhance cell survival and integrity, such as drop‐on‐demand and nozzle‐free 3D acoustic bioprinting techniques based on the principle of acoustic droplet ejection (Figure 2d‐i), [ 121 ] or based on laser‐induced forward transfer. [ 174 ] Drop‐on‐demand 3D bioprinting, which employs an electromagnetic microvalve for the precise delivery of bioink droplets with tunable volume and speed, was used to produce functional mimetic 3D corneal models with optical properties similar to those of real corneal stromal tissue using a bioink composed of collagen and agarose incorporating corneal stromal keratocytes.…”

Section: Bioprinting Of Tissue Modelsmentioning

confidence: 99%

Transformative Materials to Create 3D Functional Human Tissue Models In Vitro in a Reproducible Manner

Gerardo‐Nava,

Jansen,

Günther

et al. 2023

Adv Healthcare Materials

Self Cite

View full text Add to dashboard Cite

Recreating human tissues and organs in the petri dish to establish models as tools in biomedical sciences has gained momentum. These models can provide insight into mechanisms of human physiology, disease onset, and progression, and improve drug target validation, as well as the development of new medical therapeutics. Transformative materials play an important role in this evolution, as they can be programmed to direct cell behavior and fate by controlling the activity of bioactive molecules and material properties. Using nature as an inspiration, scientists are creating materials that incorporate specific biological processes observed during human organogenesis and tissue regeneration. This article presents the reader with state‐of‐the‐art developments in the field of in vitro tissue engineering and the challenges related to the design, production, and translation of these transformative materials. Advances regarding (stem) cell sources, expansion, and differentiation, and how novel responsive materials, automated and large‐scale fabrication processes, culture conditions, in situ monitoring systems, and computer simulations are required to create functional human tissue models that are relevant and efficient for drug discovery, are described. This paper illustrates how these different technologies need to converge to generate in vitro life‐like human tissue models that provide a platform to answer health‐based scientific questions.

show abstract