Biopriming of maize seeds with plant growth-promoting bacteria isolated from the earthworm Aporrectodea molleri: effect on seed germination and seedling growth

Abstract

Earthworms have become a potential source of multi‐beneficial bacteria and effective bioinoculants. Seed biopriming is an efficient inoculation method to apply bacteria prior to sowing, which enhances the chances of bacterial candidates to colonize the rhizosphere and/or establish a liaison with the plant. In this study, we evaluated plant growth‐promoting traits of bacterial strains isolated from the earthworm’s Aporrectodea molleri chloragogenous tissue. In addition, we investigated their prospective use as biopriming agents to enhance Zea mays germination and seedling growth. Results were subjected to principal component analysis for potential correlations between the studied parameters. The bacterial strains displayed different in vitro plant growth‐promoting characteristics and were efficient when applied in vivo as they significantly increased maize germination rate (26–78%), root elongation (67–84%), seedlings fresh weight and dry weight. Aeromonas encheleia TC22 was the most significant strain to influence germination due to its high ability to produce indole‐3‐acetic acid, and along with Pseudomonas azotoformans TC1, they were the most proficient at enhancing seedling root elongation and biomass, which was significantly correlated with their in vitro plant growth‐promoting traits. Our findings indicate that isolates TC22 and TC1 are potent bio‐primers for maize seeds and should be tested further for their use as biopriming inoculants.

Influence of Fertilization Methods and Types on Wheat Rhizosphere Microbiome Community and Functions

“…These effects may be related to how these rhizobacterial strains express multiple plant growthpromoting traits, including N 2 fixation, production of indole-3acetic acid, solubilization of nutrients (P, K, and Zn), and production of ammonia, siderophores, ACC deaminase, catalases, lipases, cellulases, and proteases. 51 More research is necessary to clarify the effects of PGPR, but in the future using growth-promoting bacteria could promote sustainable intensification of crop production and limit environmental pollution resulting from excessive nutrient application. [1][2][3]38 Journal of Agricultural and Food Chemistry…”

“…azotoformans and P. cedrina are PGPR, such as tomato, corn, wheat, and Jerusalem artichoke plants . It also plays an active role in biotic , and abiotic ,− stresses.…”

“…These bacteria include the genera mainly identified as P. azotoformans and P. cedrina. Many studies have shown that P. azotoformans and P. cedrina are PGPR, such as tomato, 51 corn, 52 wheat, 53 and Jerusalem artichoke plants. 54 It also plays an active role in biotic 51,55 and abiotic 51,56−58 stresses.…”

Plant Growth-Promoting Bacterium from Non-Agricultural Soil Improves Okra Plant Growth

“…Various microbial groups, including Aeromonas encheleia and Pseudomonas azotoformans isolated from earthworms, are affiliated with PGPB [7]. The biostimulation properties of PGPB isolated from Al-Ahsa, Saudi Arabia, have been investigated.…”

Biopriming benih dalam meningkatkan pertumbuhan dan hasil tanaman kacang tanah (Arachis hypogeae L.)

“…Oleh karena itu, diperlukan teknologi yang tepat guna berupa biopriming benih untuk menghasilkan benih bervigor tinggi yang selanjutnya mampu meningkatkan pertumbuhan dan hasil tanaman. Perlakuan benih menggunakan teknik biopriming benih dilaporkan mampu meningkatkan mutu benih (Hussain et al, 2019;Houida et al, 2022). Selain meningkatkan mutu benih, perlakuan biopriming juga dilaporkan mampu meningkatkan pertumbuhan dan hasil tanaman (Mudi et al, 2021).…”