Biopriming in bean seeds

Abstract

The incorporation of biological control organisms in seeds may help control pathogens and improve seedling performance. The aim of this study was to determine the effects of biopriming bean seeds with Trichoderma spp. and Bacillus subtilis using physiological conditioning, suspensions of biological structures and film coating techniques. Biopriming treatments with suspensions of biological structures were performed using the commercial products Agrotrich plus ® and Rhizoliptus ® . Water restriction was achieved using a PDA + Manitol (−0.7 MPa) medium for both Trichoderma spp. and Bacillus subtilis. Fifty disinfected bean seeds were placed in each petri dish. When the first radicle protrusion appeared in a seed, the other seeds were removed and dried in the laboratory environment for 48 h. Film coating was performed with the addition of the Color Seed ® (150 mL kg −1 ) polymer to a treatment solution containing either the Trichoderma spp. or the Rhizoliptus product. Seeds were dried for 48 h in the laboratory. Seeds were covered and treated with one or both organisms. Biopriming with spore or bacterial cell suspensions promoted bean seedling growth, and the other techniques were not required to potentiate these benefits. Covering the primed bean seeds reduced seed quality. Both Trichoderma spp. and Bacillus subtilis promote bean seedling growth, benefitting the seedling even after it becomes independent of the seed reserves. ARTICLE HISTORY

Métodos de inoculação de Colletotrichum lindemuthianum em sementes de feijão e danos em plântulas

“…Metodologias de inoculação são primordiais para o desenvolvimento de pesquisas relacionadas à avaliação de resistência genética e ao desenvolvimento de métodos de controle (GALLI et al, 2007;CORRÊA et al, 2008;GARCIA;JULIATTI, 2012;JUNGES et al, 2015). Os métodos de inoculação devem garantir a reprodução e expressão dos sintomas típicos da doença nas plântulas ou plantas (FALCÃO et al, 2005;SOUSA et al, 2008).…”

Determinação Do Fator De Efetividade Para Enzimas Imobilizadas Usando Métodos De Regressão Simbólica via Programação Genética

“…However, in this study, fresh and dry mass of plants at the beginning of infection was not affected (data not shown), even with the increase of protein levels and enzyme activities. One of the reasons may the fact that species of Trichoderma act as growth promoter increasing the fresh and dry mass of plants (CHACÓN et al, 2007) and seedlings (JUNGES, et al, 2015).…”

Recent Advances in Seed Enhancements

“…Besides diseases control, the application of PGPR as a biopriming agent for biofertilization is an attractive option to reduce the use of chemical fertilizers [80,81]. PGPR that have been tested as co-inoculants with rhizobia include strains of the following rhizobacteria: Azotobacter [82], Azospirillum [83], Bacillus [84], Pseudomonas [85,86], Serratia [86] and Streptomyces [87].…”

“…under stress conditions over control. Very recently, Junges et al [84] compared the potential of biopriming (Trichoderma and Bacillus spp.) with commercial available products Agrotrich plus ® and Rhizoliptus ® for enhancing growth and yield of beans.…”