Abstract:Ureolysis-driven microbially induced carbonate precipitation (MICP) is a naturally occurring process facilitated through microbial activities and biogeochemical reactions to produce calcium carbonate (CaCO 3) mineral. MICP serves as an alternative ground improvement binder method to conventional technologies which is sustainable, requires low energy for its treatment process, results in a minimal carbon footprint and could offer economic benefits. In the last two decades, MICP has drawn great interest from the… Show more
“…The effectiveness of MICP for soil stabilisation depends on several factors including the type of bacteria used, nutrient concentrations, condition favourability, approach, and soil properties [40][41][42][43]. The ureolytic bacteria Bacillus pasteurii is the most commonly used bacteria for MICP due to its high production of the urease enzyme that drives urea hydrolysis [44].…”
Section: Microbially Induced Calcite Precipitation In Soilmentioning
In line with the recent promotion of biocementation as an environmentally friendly ground improvement method, this study presents an investigation into microbially induced calcite precipitation (MICP) as a method of improving the engineering properties of soft clay. Bacillus pasteurii bacterium in vegetative cell and bacterial spore forms were used to induce MICP in clay specimens. Untreated and treated clay specimens were tested for their mechanical properties and microstructures through unconfined compression (UC) tests, free-free resonance (FFR) tests, X-ray diffraction (XRD) tests, and scanning electron microscopy with energy dispersive X-ray (SEM/EDX) tests. Results showed that both vegetative cells and bacterial spores can effectively enhance the strength and modulus of clays by inducing MICP to generate calcite crystals. Clays treated with vegetative cells exhibited earlier improvements in their strength than clays treated with bacterial spores due to earlier activity availability; however, the clays treated with bacterial spores exhibited greater strength improvements in the long term. Bacterial spores may also prove more convenient to use in geotechnical engineering practice.
“…The effectiveness of MICP for soil stabilisation depends on several factors including the type of bacteria used, nutrient concentrations, condition favourability, approach, and soil properties [40][41][42][43]. The ureolytic bacteria Bacillus pasteurii is the most commonly used bacteria for MICP due to its high production of the urease enzyme that drives urea hydrolysis [44].…”
Section: Microbially Induced Calcite Precipitation In Soilmentioning
In line with the recent promotion of biocementation as an environmentally friendly ground improvement method, this study presents an investigation into microbially induced calcite precipitation (MICP) as a method of improving the engineering properties of soft clay. Bacillus pasteurii bacterium in vegetative cell and bacterial spore forms were used to induce MICP in clay specimens. Untreated and treated clay specimens were tested for their mechanical properties and microstructures through unconfined compression (UC) tests, free-free resonance (FFR) tests, X-ray diffraction (XRD) tests, and scanning electron microscopy with energy dispersive X-ray (SEM/EDX) tests. Results showed that both vegetative cells and bacterial spores can effectively enhance the strength and modulus of clays by inducing MICP to generate calcite crystals. Clays treated with vegetative cells exhibited earlier improvements in their strength than clays treated with bacterial spores due to earlier activity availability; however, the clays treated with bacterial spores exhibited greater strength improvements in the long term. Bacterial spores may also prove more convenient to use in geotechnical engineering practice.
“…As an alternative, microbial-induced carbonate precipitation (MICP), a newly developed soil improvement technology, has drawn a great deal of interest among researchers (Achal and Kawasaki, 2016;Ivanov et al, 2019;Omoregie et al, 2020). The mechanism of MICP involves nonpathogenic bacteria and their metabolism for stabilization, in a way similar to naturally occurring biomineralization process.…”
Peat is one of the most challenging and problematic soils in the fields of geotechnical and environmental engineering. The most critical problems related to peat soils are extremely low strength and high compressibility, resulting in poor inhabitancy and infrastructural developments in their vicinity. Thus far, peat soils were stabilized using Portland cement; however, the production of Portland cement causes significant emission of greenhouse gases, which is not environmentally desirable. Microbial-induced carbonate precipitation (MICP) is an innovative technique for improving the mechanical properties of soil through potentially environmentally friendly processes. This article presents a laboratory study carried out with the aim of investigating the viability and effect of scallop shell powder (SSP) on enhancing the mechanical properties of the MICP-treated amorphous peat. The hypothesis was that the distribution of SSP (as-derived calcite particles) would (i) provide more nucleation sites to precipitates and (ii) increase the connectivity of MICP bridges to facilitate mineral skeleton to amorphous peat, accompanied by an increase in its compressive strength. Specimens were treated at varying combinations of SSP and MICP reagents, and the improvement was comprehensively assessed through a series of unconfined compression tests and supported by microscale and chemical analyses such as scanning electron microscopy, energy-dispersive X-ray analysis, and X-ray diffraction analysis. The outcomes showed that incorporating SSP in MICP treatment would be a promising approach to treat amorphous peat soils. The proposed approach could improve the unconfined compressive strength by over 200% after a 7-day curing period, while the conventional MICP could not exhibit any significant improvements.
“…This result confirms the observations of Dhami et al [86] that the urease pathway is faster and more efficient than the CA pathway in terms of extracellular enzyme production and calcium carbonate precipitation. Although the CA pathway was demonstrated herein to be less efficient in CaCO 3 production overall, this metabolism has a substantial advantage over the urease pathway, namely the absence of ammonia production, a toxic molecule that could limit the use of the ureolytic pathway [87].…”
Section: Analysis Of Biocalcification Capacitymentioning
Marine bacterial biomineralisation by CaCO3 precipitation provides natural limestone structures, like beachrocks and stromatolites. Calcareous deposits can also be abiotically formed in seawater at the surface of steel grids under cathodic polarisation. In this work, we showed that this mineral-rich alkaline environment harbours bacteria belonging to different genera able to induce CaCO3 precipitation. We previously isolated 14 biocalcifying marine bacteria from electrochemically formed calcareous deposits and their immediate environment. By microscopy and µ-Raman spectroscopy, these bacterial strains were shown to produce calcite-type CaCO3. Identification by 16S rDNA sequencing provided between 98.5 and 100% identity with genera Pseudoalteromonas, Pseudidiomarina, Epibacterium, Virgibacillus, Planococcus, and Bhargavaea. All 14 strains produced carbonic anhydrase, and six were urease positive. Both proteins are major enzymes involved in the biocalcification process. However, this does not preclude that one or more other metabolisms could also be involved in the process. In the presence of urea, Virgibacillus halodenitrificans CD6 exhibited the most efficient precipitation of CaCO3. However, the urease pathway has the disadvantage of producing ammonia, a toxic molecule. We showed herein that different marine bacteria could induce CaCO3 precipitation without urea. These bacteria could then be used for eco-friendly applications, e.g., the formation of bio-cements to strengthen dikes and delay coastal erosion.
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