Biopolymeric Materials Used as Nonviral Vectors: A Review

Abstract

Bacterial transformation and gene transfection can be understood as being the results of introducing specific genetic material into cells, resulting in gene expression, and adding a new genetic trait to the host cell. Many studies have been carried out to investigate different types of lipids and cationic polymers as promising nonviral vectors for DNA transfer. The present study aimed to carry out a systematic review on the use of biopolymeric materials as nonviral vectors. The methodology was carried out based on searches of scientific articles and applications for patents published or deposited from 2006 to 2020 in different databases for patents (EPO, USPTO, and INPI) and articles (Scopus, Web of Science, and Scielo). The results showed that there are some deposits of patents regarding the use of chitosan as a gene carrier. The 16 analyzed articles allowed us to infer that the use of biopolymers as nonviral vectors is limited due to the low diversity of biopolymers used for these purposes. It was also observed that the use of different materials as nonviral vectors is based on chemical structure modifications of the material, mainly by the addition of cationic groups. Thus, the use of biopolymers as nonviral vectors is still limited to only a few polysaccharide types, emphasizing the need for further studies involving the use of different biopolymers in processes of gene transfer.

Effect of high hydrostatic pressure on the in vitro development and molecular quality of transgenic rabbit embryos derived from nano-transfected zygotes

“…In order to improve transgenesis efficiency, new carriers for nucleic acids are strongly desired. Nanoparticles, which combine a small size, a capability to bind and transfer substances inside cells, and a low toxicity, seem to be promising as new gene carriers (Santos et al, 2021). However, there are not many data considering their cytotoxicity and potential efficiency in terms of zygote transfection.…”