Biopolymer Molecular Weight Can Modulate the Wound Healing Efficacy of Multivalent Sonic Hedgehog–Hyaluronic Acid Conjugates

Holstlaw, Taylor A.; Mahomed, Mavish; Brier, Livia W.; Young, David M.; Boudreau, Nancy; Jackson, Wesley M.

doi:10.1021/acs.biomac.7b00553

Cited by 6 publications

(3 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Multimodal imaging-guided multistage targeted chemo-photothermal therapy [335] Optimized HA-methotrexate conjugates OA therapy [336] Steroids Steroid grafted on to HA Antioxidant delivery [337] EGF HA-EGF conjugate Chronic wound healing [338] Skin wound healing and regeneration [339] Anti-tumor necrosis factor-α Anti-tumor necrosis factor-α HA conjugate Early healing effects in a rat burn model [340] Epigallocatechin-3-O-Gallate HA-Epigallocatechin-3-O-Gallate conjugates injectable hydrogel Free radical scavenging [341] Sonic hedgehog Sonic hedgehog-HA conjugates Wound healing [342] Multivalent Sonic hedgehog conjugate Diabetic wound healing [343] Catechin Hyaluronic acid-green tea catechin nanogels Targeted intracellular protein delivery [344] Quercetin HA-quercetin conjugated micelles A tumor cell-targeted prodrug [345] Opiods HA-opioid conjugate Controlled delivery treatment for pain alleviation applications [346] Adv. Therap.…”

Section: Methotrexate Coated Magnetic Polydopamine Nanoparticlesmentioning

confidence: 99%

Glycosaminoglycan and Proteoglycan Biotherapeutics in Articular Cartilage Protection and Repair Strategies: Novel Approaches to Visco‐supplementation in Orthobiologics

Hayes

Melrose

2019

Advanced Therapeutics

View full text Add to dashboard Cite

The aim of this study is to review developments in glycosaminoglycan and proteoglycan research relevant to cartilage repair biology and in particular the treatment of osteoarthritis (OA). Glycosaminoglycans decorate a diverse range of extracellular matrix and cell associated proteoglycans conveying structural organization and physico-chemical properties to tissues. They play key roles mediating cellular interactions with bioactive growth factors, cytokines, and morphogenetic proteins, and structural fibrillar collagens, cell interactive and extracellular matrix proteoglycans, and glycoproteins which define tissue function. Proteoglycan degradation detrimentally affects tissue functional properties. Therapeutic strategies have been developed to counter these degenerative changes. Neo-proteoglycans prepared from chondroitin sulfate or hyaluronan and hyaluronan or collagen-binding peptides emulate the interactive, water imbibing, weight bearing, and surface lubricative properties of native proteoglycans. Many neo-proteoglycans outperform native proteoglycans in terms of water imbibition, matrix stabilization, and resistance to proteolytic degradation. The biospecificity of recombinant proteoglycans however, provides precise attachment to native target molecules. Visco-supplements augmented with growth factors/therapeutic cells, hyaluronan, and lubricin (orthobiologicals) have the capacity to lubricate and protect cartilage, control inflammation, and promote cartilage repair and regeneration of early cartilage lesions and may represent a more effective therapeutic approach to the treatment of mild to moderate OA and deserve further study.Similar protective perineural net structures assembled from the lectican proteoglycan family and HA also occur in the CNS/PNS. These so called perineuronal nets are visualized by immunolocalization of MAb 1B5 (+) epitope in rat brain (e) and pericellular 1B5(+) epitope expression by isolated neurons (f). Scale bars 100 µm.

show abstract

Section: Methotrexate Coated Magnetic Polydopamine Nanoparticlesmentioning

confidence: 99%

Glycosaminoglycan and Proteoglycan Biotherapeutics in Articular Cartilage Protection and Repair Strategies: Novel Approaches to Visco‐supplementation in Orthobiologics

Hayes

Melrose

2019

Advanced Therapeutics

View full text Add to dashboard Cite

show abstract

“…Similarly, recent other studies have demonstrated that HA conjugation could increase the activity of proteins. 18,[22][23][24][25][26][27] The cytokine neutralizing HA-antibody conjugates appeared to strengthen the binding affinity for inflammation mediators with the simultaneous activation of CD44/ RHAMM and the suppression of cytokine-signaling networks. 25 The used HA with a MW of 100-300 kDa was also reported to promote wound healing.…”

Section: Effect Of Ha-egf Conjugates On Hacat Cellsmentioning

confidence: 99%

Synergistic effects of hyaluronate – epidermal growth factor conjugate patch on chronic wound healing

et al. 2018

View full text Add to dashboard Cite

The proteolytic microenvironment in the wound area reduces the stability and the half-life of growth factors in vivo, making difficult the topical delivery of growth factors. Here, epidermal growth factor (EGF) was conjugated to hyaluronate (HA) to improve the long-term stability against enzymatic degradation and the therapeutic effect by enhancing the biological interaction with HA receptors on skin cells. After the synthesis of HA-EGF conjugates, they were incorporated into a patch-type formulation for the facile topical application and sustained release of EGF. According to ELISA, the HA-EGF conjugates showed a long-term stability compared with native EGF. Furthermore, HA-EGF conjugates appeared to interact with skin cells through two types of HA and EGF receptors, resulting in a synergistically improved healing effect. Taken together, we could confirm the feasibility of HA-EGF conjugates for the transdermal treatment of chronic wounds.

show abstract

“…The protocol for synthesizing HA-SpyTag is a combination of previous methods. [22][23][24] Every step was performed at 25 °C unless stated. 400 mg of hyaluronic acid (HA) (242 kDa, sodium salt, Lifecore Biomedical LLC) was dissolved in 40 mL ddH2O.…”

Section: Introductionmentioning

confidence: 99%

Assembling and decorating hyaluronan hydrogels with twin protein superglues to mimic cell-cell interactions

Wieduwild

Howarth

2018

Biomaterials

View full text Add to dashboard Cite

Simple polymeric scaffolds have yielded dramatic effects on cell behavior. For more sophisticated phenotypes, precise and efficient chemistries are desired to incorporate proteins into these scaffolds. Here we derivatize hyaluronan with an elastin-like polypeptide containing telechelic SpyTags (HA-SpyTag). Our second network component, the TriCatcher protein, had two SpyCatchers and a terminal SnoopCatcher. Mixing HA-SpyTag with TriCatcher led to rapid hydrogel formation, via spontaneous amidation. SnoopCatcher allowed modular network decoration with SnoopTagJr-linked adhesion molecules, through orthogonal transamidation. This programmed scaffold enables the testing of how individual matrix-anchored protein interactions affect cell behavior. Epithelial cell adhesion molecule (EpCAM) regulates cell behavior and migration, with important effects in cancer. EpCAM-anchoring to the hydrogel induced disassembly of non-malignant mammary spheres in 3D culture. Integrating signaling proteins into biomaterials via efficient biocompatible chemistry should reveal key cues to control cell behavior.

show abstract

Biopolymer Molecular Weight Can Modulate the Wound Healing Efficacy of Multivalent Sonic Hedgehog–Hyaluronic Acid Conjugates

Cited by 6 publications

References 42 publications

Glycosaminoglycan and Proteoglycan Biotherapeutics in Articular Cartilage Protection and Repair Strategies: Novel Approaches to Visco‐supplementation in Orthobiologics

Glycosaminoglycan and Proteoglycan Biotherapeutics in Articular Cartilage Protection and Repair Strategies: Novel Approaches to Visco‐supplementation in Orthobiologics

Synergistic effects of hyaluronate – epidermal growth factor conjugate patch on chronic wound healing

Assembling and decorating hyaluronan hydrogels with twin protein superglues to mimic cell-cell interactions

Contact Info

Product

Resources

About