Biophysics of sphingolipids II. Glycosphingolipids: An assortment of multiple structural information transducers at the membrane surface

Maggio, Bruno; Fanani, María Laura; Rosetti, Carla M.; Wilke, Natalia

doi:10.1016/j.bbamem.2006.04.020

Cited by 64 publications

(68 citation statements)

References 246 publications

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“…Sphingolipids are ubiquitous components of animal cell membranes and are also associated with intracellular organelles [14]. They have been found to play an important role in both intracellular and extra-cellular signaling pathways and are a key component of membrane rafts or microdomains [15].…”

Section: Sphingolipid Classificationmentioning

confidence: 99%

Sphingolipid therapy in myocardial ischemia–reperfusion injury

Gundewar

Lefer

2008

Biochimica et Biophysica Acta (BBA) - General Subjects

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Sphingolipids are known to play a significant physiological role in cell growth, cell differentiation, and critical signal transduction pathways. Recent studies have demonstrated a significant role of sphingolipids and their metabolites in the pathogenesis of myocardial ischemia-reperfusion injury. Our laboratory has investigated the cytoprotective effects of N,N,N-Trimethylsphingosine chloride (TMS), a stable N-methylated synthetic sphingolipid analogue on myocardial and hepatic ischemia reperfusion injury in clinically relevant in vivo murine models of ischemia-reperfusion injury. TMS administered intravenously at the onset of ischemia reduced myocardial infarct size in the wild-type and obese (ob/ob) mice. Following myocardial I/R, there was an improvement in cardiac function in the wild-type mice. Additionally, TMS also decreased serum liver enzymes following hepatic I/R in wild-type mice. The cytoprotective effects did not extend to the ob/ob mice following hepatic I/R or to the db/db mice following both myocardial and hepatic I/R. Our data suggests that although TMS is cytoprotective following I/R in normal animals, the cytoprotective actions of TMS are largely attenuated in obese and diabetic animals which may be due to altered signaling mechanisms in these animal models. Here we review the therapeutic role of TMS and other sphingolipids in the pathogenesis of myocardial ischemia reperfusion injury and their possible mechanisms of cardioprotection.

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Section: Sphingolipid Classificationmentioning

confidence: 99%

Sphingolipid therapy in myocardial ischemia–reperfusion injury

Gundewar

Lefer

2008

Biochimica et Biophysica Acta (BBA) - General Subjects

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“…Models of mammalian SC have suggested that the covalently bound and intercellular lipids interdigitate at their respective head groups to create links between adjacent corneocytes . However, if cerebrosides are packed too tightly together, the lamellar structure breaks down (Maggio et al, 2006). Therefore, if intercellular lipids and CBLs interdigitate in avian SC, the cerebrosides must be spaced far enough apart to prevent disruptions of the lamellar structure.…”

Section: Discussionmentioning

confidence: 99%

Lipid composition of the stratum corneum and cutaneous water loss in birds along an aridity gradient

Champagne

Muñoz‐Garcia

Shtayyeh

et al. 2012

Journal of Experimental Biology

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SUMMARYIntercellular and covalently bound lipids within the stratum corneum (SC), the outermost layer of the epidermis, are the primary barrier to cutaneous water loss (CWL) in birds. We compared CWL and intercellular SC lipid composition in 20 species of birds from desert and mesic environments. Furthermore, we compared covalently bound lipids with CWL and intercellular lipids in the lark family (Alaudidae). We found that CWL increases in birds from more mesic environments, and this increase was related to changes in intercellular SC lipid composition. The most consistent pattern that emerged was a decrease in the relative amount of cerebrosides as CWL increased, a pattern that is counterintuitive based on studies of mammals with Gaucher disease. Although covalently bound lipids in larks did not correlate with CWL, we found that covalently bound cerebrosides correlated positively with intercellular cerebrosides and intercellular cholesterol ester, and intercellular cerebrosides correlated positively with covalently bound free fatty acids. Our results led us to propose a new model for the organization of lipids in the avian SC, in which the sugar moieties of cerebrosides lie outside of intercellular lipid layers, where they may interdigitate with adjacent intercellular cerebrosides or with covalently bound cerebrosides.

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“…sphingomyelin) enrichment. Despite the reports saying that sphingomyelin is more easily solubilized than phosphatidylcholine (Helenius and Simons 1975;Sot et al 2002) and that self-aggregation of sphingolipids is not favorable (Uragami et al 2001;Maggio et al 2006), TX-100-DRMs obtained from intact erythrocytes are enriched in sphingomyelin (Casadei et al 2014b). Curiously, Brij 98-and Brij 58-DRMs were also enriched in cholesterol but they did not show increased order parameters, as observed for TX-100 and -C 1 2 E 8 DR Ms. Brij-DRMs were not enriched in sphingomyelin and exhibited a phospholipid composition very similar to that observed in erythrocyte membrane ghosts (Casadei et al 2014b).…”

Section: Order Parameter Of Membrane Lipids Resistant To Detergentsmentioning

confidence: 99%

Biophysical approaches in the study of biomembrane solubilization: quantitative assessment and the role of lateral inhomogeneity

et al. 2017

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Detergents are amphiphilic molecules widely used to solubilize biological membranes and/or extract their components. Nevertheless, because of the complex composition of biomembranes, their solubilization by detergents has not been systematically studied. In this review, we address the solubilization of erythrocytes, which provide a relatively simple, robust and easy to handle biomembrane, and of biomimetic models, to stress the role of the lipid composition on the solubilization process. First, results of a systematic study on the solubilization of human erythrocyte membranes by different series of non-ionic (Triton, CxEy, Brij, Renex, Tween), anionic (bile salts) and zwitterionic (ASB, CHAPS) detergents are shown. Such quantitative approach allowed us to propose R e sat -the effective detergent/lipid molar ratio in the membrane for the onset of hemolysis as a new parameter to classify the solubilization efficiency of detergents. Second, detergent-resistant membranes (DRMs) obtained as a result of the partial solubilization of erythrocytes by TX-100, C 12 E 8 and Brij detergents are examined. DRMs were characterized by their cholesterol, sphingolipid and specific proteins content, as well as lipid packing. Finally, lipid bilayers of tuned lipid composition forming liposomes were used to investigate the solubilization process of membranes of different compositions/phases induced by Triton X-100. Optical microscopy of giant unilamellar vesicles revealed that pure phospholipid membranes are fully solubilized, whereas the presence of cholesterol renders the mixture partially or even fully insoluble, depending on the composition. Additionally, Triton X-100 induced phase separation in raft-like mixtures, and selective solubilization of the fluid phase only.

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Biophysics of sphingolipids II. Glycosphingolipids: An assortment of multiple structural information transducers at the membrane surface

Cited by 64 publications

References 246 publications

Sphingolipid therapy in myocardial ischemia–reperfusion injury

Sphingolipid therapy in myocardial ischemia–reperfusion injury

Lipid composition of the stratum corneum and cutaneous water loss in birds along an aridity gradient

Biophysical approaches in the study of biomembrane solubilization: quantitative assessment and the role of lateral inhomogeneity

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