Biophysical study of the effect of ovalbumin and lysozyme in DMPC/sphingomyelin/cholesterol bilayers

The disordering effect of SARMs on a biomembrane model

“…35–37 It has been reported that the drop evaporation process leads to the assembly of phospholipid mixtures for liposome preparation 35 and we have taken advantage of this methodology to successfully assemble biohybrid systems as described in previous works. 37–39 Here, to obtain DMPC/SARMs films, DMPC, and SARMs were weighted and hydrated together with deionized water at 40 °C under stirring (100–1000 rpm) and mixed for 5 h (1000 rpm) at 40 °C. After that, droplets of the DMPC/SARMs suspension were subjected to a drop evaporation process to generate DMPC/SARMs films.…”

Biophysical investigation of liposome systems decorated with bioconjugated copolymers in the presence of amantadine

“…This capability has been previously observed in other biological systems where lipid domains can function as protein-anchoring platforms, 35,36,38 and along these lines, we have recently reported important features derived from non-specific lipid–protein interactions of a lipid model composed of 1,2-dimyristoyl- sn-glycero -3-phosphocholine (DMPC), sphingomyelin (SM), and cholesterol (chol) under the influence of lysozyme and ovalbumin. 40 Thereafter, we combined the peptide-functionalized block -copolymers with large unilamellar vesicles (LUVs) made of DMPC/SM/chol (herein denoted as MSC) to obtain biohybrid liposomes decorated with such bioconjugated copolymers, where the MSC lipid mixture was employed as a platform for anchoring the bioconjugated copolymers. Then, biophysical properties of such biohybrid liposomes decorated with bioconjugated copolymers were thoroughly evaluated by means of differential scanning calorimetry (DSC), dynamic light scattering (DLS), cryogenic transmission electron microscopy (cryoTEM), cytotoxicity assays, and fluorescence microscopy (Scheme 1).…”