Biophysical studies with AICD-47 reveal unique binding behavior characteristic of an unfolded domain

Das, Samir; Ghosh, Saptaparni; Dasgupta, Dipak; Sen, U.; Mukhopadhyay, Debashis

doi:10.1016/j.bbrc.2012.07.067

Cited by 2 publications

(1 citation statement)

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“…In the 3DXC binary complex dimer of AICD with Fe65, however, the first soliton is held in place because of interactions with Fe65. Current work is involved to develop and apply our approach to analyze and understand conformational transitions in proteins, in particular amyloid formation, as in AICD (Das et al 2012), Fe65 and A β 42.…”

Section: Solitons and Protein Foldingmentioning

confidence: 99%

My 65 years in protein chemistry

Scheraga

2015

Quart. Rev. Biophys.

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This is a tour of a physical chemist through 65 years of protein chemistry from the time when emphasis was placed on the determination of the size and shape of the protein molecule as a colloidal particle, with an early breakthrough by James Sumner, followed by Linus Pauling and Fred Sanger, that a protein was a real molecule, albeit a macromolecule. It deals with the recognition of the nature and importance of hydrogen bonds and hydrophobic interactions in determining the structure, properties, and biological function of proteins until the present acquisition of an understanding of the structure, thermodynamics, and folding pathways from a linear array of amino acids to a biological entity. Along the way, with a combination of experiment and theoretical interpretation, a mechanism was elucidated for the thrombin-induced conversion of fibrinogen to a fibrin blood clot and for the oxidative-folding pathways of ribonuclease A. Before the atomic structure of a protein molecule was determined by x-ray diffraction or nuclear magnetic resonance spectroscopy, experimental studies of the fundamental interactions underlying protein structure led to several distance constraints which motivated the theoretical approach to determine protein structure, and culminated in the Empirical Conformational Energy Program for Peptides (ECEPP), an all-atom force field, with which the structures of fibrous collagen-like proteins and the 46-residue globular staphylococcal protein A were determined. To undertake the study of larger globular proteins, a physics-based coarse-grained UNited-RESidue (UNRES) force field was developed, and applied to the protein-folding problem in terms of structure, thermodynamics, dynamics, and folding pathways. Initially, single-chain and, ultimately, multiple-chain proteins were examined, and the methodology was extended to protein–protein interactions and to nucleic acids and to protein–nucleic acid interactions. The ultimate results led to an understanding of a variety of biological processes underlying natural and disease phenomena.

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Section: Solitons and Protein Foldingmentioning

confidence: 99%