Biophysical Studies of the Structure, Stability, and Ligand Binding Properties of G-Quadruplex DNA: Thoughts and Comparisons of the K-ras, c-MYC, and Bcl-2 Oncogene Promoter Sequence Quadruplexes

Dettler, Jamie M.; Lewis, Edwin A.

doi:10.1021/bk-2011-1082.ch003

Cited by 2 publications

(1 citation statement)

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“…The CD profile of k-RAS and c-KIT2 in PBS at pH 7.4 displayed characteristics of a parallel topology, with large positive Cotton effects around 260 nm, and smaller negative peaks at shorter wavelengths. The folding of k-RAS and c-KIT2 into parallel conformations is consistent with other studies reported in the literature [6,65,66,67,68]. The only telomeric sequence in this study—h-TELO—folded in a hybrid manner, and its CD fingerprint was characterized by a positive peak at 290 nm and a small negative peak at 260 nm.…”

Section: Resultssupporting

confidence: 90%

Importance of Chiral Recognition in Designing Metal-Free Ligands for G-Quadruplex DNA

et al. 2019

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Four pairs of amino acid-functionalized naphthalenediimide enantiomers (d- and l-lysine derived NDIs) were screened toward G-quadruplex forming sequences in telomeres (h-TELO) and oncogene promoters: c-KIT1, c-KIT2, k-RAS and BCL-2. This is the first study to address the effect of point chirality toward G-quadruplex DNA stabilization using purely small organic molecules. Enantioselective behavior toward the majority of ligands was observed, particularly in the case of parallel conformations of c-KIT2 and k-RAS. Additionally, Nε-Boc-l-Lys-NDI and Nε-Boc-d-Lys-NDI discriminate between quadruplexes with parallel and hybrid topologies, which has not previously been observed with enantiomeric ligands.

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Section: Resultssupporting

confidence: 90%

Importance of Chiral Recognition in Designing Metal-Free Ligands for G-Quadruplex DNA

et al. 2019

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Calorimetry

Emerson¹,

Le²,

Lewis³

2012

Encyclopedia of Life Sciences

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In biology, particularly in studies relating the structure of biopolymers to their functions, two of the most important questions are: (1) how tightly does a small molecule bind to a specific interaction site? and (2) if the small molecule is a substrate and is converted to a product, how fast does the reaction take place? Because almost any chemical reaction or physical change is accompanied by a change in heat or enthalpy, an isothermal titration calorimeter is an ideal instrument to measure either how much of a reaction has taken place or the rate at which a reaction is occurring. In contrast to optical methods, calorimetric measurements can be done with reactants that are spectroscopically silent (a chromophore or fluorophore tag is not required), can be done on opaque, turbid or heterogeneous solutions (e.g. cell suspensions) and can be done over a range of biologically relevant conditions (temperature, salt, pH, etc ). Key Concepts: Almost all chemical reactions are accompanied by a change in heat or enthalpy. Calorimetry can determine the complete set of thermodynamic parameters that characterise binding reactions, for example K, Δ G °, Δ H °, Δ S ° and Δ C p . Binding reactions are typically pH, salt and temperature dependant.

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Biophysical Studies of the Structure, Stability, and Ligand Binding Properties of G-Quadruplex DNA: Thoughts and Comparisons of the K-ras, c-MYC, and Bcl-2 Oncogene Promoter Sequence Quadruplexes

Cited by 2 publications

References 23 publications

Importance of Chiral Recognition in Designing Metal-Free Ligands for G-Quadruplex DNA

Importance of Chiral Recognition in Designing Metal-Free Ligands for G-Quadruplex DNA

Calorimetry

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