Biophysical studies of the effect of high power ultrasound on the DNA solution

Ali, Maha; Al‐Saad, Khalid; Ali, Carmen M.

doi:10.1016/j.ejmp.2013.06.002

Cited by 7 publications

(2 citation statements)

References 39 publications

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“…Furusawa et al. have reported that damage on genetic materials can be induced low intensity ultrasound whose intensity is 0.3 W/cm 2 [68–70]. As can be observed in Figure 6, sonication of 1 W/cm 2 on pure siRNA reduced the measured amount of siRNA approximately 15%.…”

Section: Discussionmentioning

confidence: 97%

Synthesis of echogenic liposomes for sonoporation

Park

Jung

Park

et al. 2022

Micro & Nano Letters

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Ultrasound contrast agents (UCAs), which are groups of engineered microbubbles, have been recently studied for drug delivery applications, since the cavitation of bubbles can increase the temporary permeability of nearby cells. However, the internal volume of UCAs is generally filled with gas, hence loading drug molecules into UCAs is limited. In this study, an echogenic liposome with a liquid and gas core is proposed as an alternative carrier of genetic material for ultrasound‐mediated drug delivery. The structure of the synthesized echogenic liposome was analysed via transmission electron microscopy and confocal microscopy with fluorescent labels. The protection of siRNA by the echogenic liposomes was also verified by exposure to RNase. The results indicate that at least 10% of the total siRNA used in the experiment was successfully protected by the proposed echogenic liposome. Additionally, the release of siRNA from the liposomes could be successfully achieved with 1 W/cm2 ultrasound sonication at 1 MHz; parameters low enough to be used in generic ultrasound therapeutic systems. Although further studies to clarify the responses to incident ultrasound fields and to quantitatively analyse the internal liquid volume for drug loading are required, the proposed echogenic liposome has great potential for ultrasound‐mediated gene delivery.

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Section: Discussionmentioning

confidence: 97%

Synthesis of echogenic liposomes for sonoporation

Park

Jung

Park

et al. 2022

Micro & Nano Letters

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“…We used relatively low-frequency,t hatis, severaltens of kHz, ultrasound,partlysothatwe cans et thee xperimentalc onditionsi nawell-controlled manner to evaluate non-thermale ffects.L ow-frequencyu ltrasoundh as been appliedt ol ithotripsy,p hacoemulsification,u ltrasound-assisted liposuction, tissue cuttinga nd vessel sealing, ands kin permeabilization. [4,[15][16][17][18] In thesea pplications, then on-thermal effect,t hati s, cavitation effect,p lays them ajor role.A lthough severalp reviousp apersh aved iscussed thec avitation effect on DNA molecules, [19][20][21][22][23][24] there have been few studies on the quantitative evaluation of damage to "genome-sized"D NA molecules. It is important to unveil the effects of ultrasound from the standpoints of both safety and basic science.…”

Section: Introductionmentioning

confidence: 99%

Double‐Strand Breaks in Genome‐Sized DNA Caused by Ultrasound

et al. 2017

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DNA double‐strand breaks (DSBs) caused by ultrasound were evaluated in a quantitative manner by single‐molecule fluorescence microscopy. We compared the effect of time‐interval (or pulse) sonication to that of continuous wave (CW) sonication at a fixed frequency of 30 kHz. Pulses caused fewer DSBs than CW sonication under the same total input ultrasound energy when the pulse repetition period was above the order of a second. In contrast, pulses caused more DSBs than CW sonication for pulse widths shorter than a second. These effect of ultrasound on DNA were interpreted in terms of the time‐dependent decay in the probability of breakage during the duration of a pulse. We propose a simple phenomenological model by considering a characteristic decay in the probability of DSBs during single‐pulse sonication, which reproduces the essence of the experimental trend. In addition, a data analysis revealed a characteristic scaling behavior between the number of pulses and the number of DSBs.

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Molecular regulatory mechanisms of Escherichia coli O157:H7 in response to ultrasonic stress revealed by proteomic analysis

Zhang

Ashokkumar

et al. 2020

Ultrasonics Sonochemistry

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Biophysical studies of the effect of high power ultrasound on the DNA solution

Cited by 7 publications

References 39 publications

Synthesis of echogenic liposomes for sonoporation

Synthesis of echogenic liposomes for sonoporation

Double‐Strand Breaks in Genome‐Sized DNA Caused by Ultrasound

Molecular regulatory mechanisms of Escherichia coli O157:H7 in response to ultrasonic stress revealed by proteomic analysis

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