Biophysical Responses upon the Interaction of Nanomaterials with Cellular Interfaces

Wu, Yun; Putcha, Nirupama; Ng, Kee Woei; Leong, David Tai; Lim, Chwee Teck; Loo, Say Chye Joachim; Chen, Xiaodong

doi:10.1021/ar300046u

Cited by 128 publications

(97 citation statements)

References 57 publications

(103 reference statements)

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“…As NPs can interact with cultured cells through a wide variety of different mechanisms [49], it is important to verify the functionality of the cells after cell labeling. For C17.2 and PC12 cells, the functionality of the cells was not investigated by their cellular spreading, as these cells are more rounded and have a less well-defined cytoskeleton, which hardens any straightforward analysis.…”

Section: Effect Of Qdots On Cell Functionalitymentioning

confidence: 99%

The performance of gradient alloy quantum dots in cell labeling

Soenen¹,

Manshian²,

Himmelreich³

et al. 2014

Biomaterials

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The interest in using quantum dots (QDots) as highly fluorescent and photostable nanoparticles in biomedicine is vastly increasing. One major hurdle that slows down the (pre)clinical translation of QDots is their potential toxicity. Several strategies have been employed to optimize common coreshell QDots, such as the use of gradient alloy (GA)-QDots. These particles no longer have a sizedependent emission wavelength, but the emission rather depends on the chemical composition of the gradient layer. Therefore, particles of identical sizes but with emission maxima spanning the entire visible spectrum can be generated. In the present study, two types of GA-QDots are studied with respect to their cytotoxicity and cellular uptake. A multiparametric cytotoxicity approach reveals concentration-dependent effects on cell viability, oxidative stress, cell morphology and cell functionality (stem cell differentiation and neurite outgrowth), where the particles are very robust against environmentally-induced breakdown. Non-toxic concentrations are defined and compared to common core-shell QDots analyzed under identical conditions. Additionally, this value is translated into a functional value by analyzing the potential of the particles for cell visualization. Interestingly, these particles result in clear endosomal localization, where different particles result in identical intracellular distributions. This is in contrast with CdTe QDots with the same surface coating, which resulted in clearly distinct intracellular distributions as a result of differences in nanoparticle diameter.The GA-QDots are therefore ideal platforms for cell labeling studies given their high brightness, low cytotoxicity and identical sizes, resulting in highly similar intracellular particle distributions which offer a lot of potential for optimizing drug delivery strategies.

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Section: Effect Of Qdots On Cell Functionalitymentioning

confidence: 99%

The performance of gradient alloy quantum dots in cell labeling

Soenen¹,

Manshian²,

Himmelreich³

et al. 2014

Biomaterials

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“…The expressions of FAK, RhoA and Rac1 at 12 h were at similar levels on Planar, Nano and Nano-NH 2 . After 60 h, the expressions of FAK and RhoA were upregulated for the cells on Nano and Nano-NH 2 (Figures 6c and e). Therefore, we hypothesize that the upright nanosheets may promote the delivery of naked plasmid (in this case, GATA4) by enhancing the expression of integrins on the cell membrane.…”

Section: Resultsmentioning

confidence: 97%

“…1 The NPs with positive charge bind to the gelled areas of the cell membrane to induce fluidity for particle penetration and disrupt the intracellular microtubule assembly. 2,3 In addition to the vector-based delivery, a few physical or mechanical methods (for example, microinjection, gene gun, electroporation, sonoporation and laser irradiation) are used to deliver naked plasmid DNA, but they can also cause cell damage. 4 It remains very challenging to deliver naked plasmid DNA without any vector or physical stimulation into normal cells and stem cells, which are difficult to transfect.…”

Section: Introductionmentioning

confidence: 99%

Nanosheet transfection: effective transfer of naked DNA on silica glass

Huang

Ariga

et al. 2015

NPG Asia Mater

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Existing gene delivery technologies using nonviral vectors or physical stimulation are limited by cytotoxicity. Here, we reveal an easy and new method to fabricate silica upright nanosheets, which can be easily scaled up and used for gene delivery. We demonstrate that naked DNA can be transferred into difficult-to-transfect cells (for example, stem cells) by plating cells on silica glass with the upright nanosheets without using any vector. Gene entry is probably achieved through the integrin-FAK-Rho signaling axis, which can be activated by the cytoskeleton rearrangement on nanosheets in a limited time frame ('transfection window'). Transfecting naked GATA4-binding protein 4 plasmids into stem cells can upregulate the other two important cardiac marker genes myocyte enhancer factor 2C and T-box 5. The transfected mesenchymal stem cells express the cardiac marker proteins at 7 days after transfection, which confirms the success of the innovative gene delivery approach. The vector-free silica nanosheet-induced transfection is simple and effective but faster and safer than the conventional technologies.

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“…The interactions between cells and biomaterials are mainly dependent on the physicochemical characteristics of the biomaterials' surfaces 16. It is expected that this nanocoating could improve surface properties and provide a special functional domain for the 3D bioprinted scaffold to promote cell–substrate interaction 17.…”

Section: Resultsmentioning

confidence: 99%

Biologically Inspired Smart Release System Based on 3D Bioprinted Perfused Scaffold for Vascularized Tissue Regeneration

et al. 2016

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A critical challenge to the development of large‐scale artificial tissue grafts for defect reconstruction is vascularization of the tissue construct. As an emerging tissue/organ manufacturing technique, 3D bioprinting offers great precision in controlling the internal architecture of a scaffold with preferable mechanical strength and printing complicated microstructures comparable to native tissue. However, current bioprinting techniques still exhibit difficulty in achieving biomimetic nano resolution and cooperating with bioactive spatiotemporal signals. In this study, a comprehensive design of engineered vascularized bone construct is presented for the first time by integrating biomimetic 3D bioprinted fluid perfused microstructure with biologically inspired smart release nanocoating, which is regarded as an aspiring concept combining engineering, biological, and material science. In this biologically inspired design, angiogenesis and osteogenesis are successively induced through a matrix metalloprotease 2 regulative mechanism by delivering dual growth factors with sequential release in spatiotemporal coordination. Availability of this system is evaluated in dynamic culture condition, which is similar to fluid surrounding in vivo, as an alternative animal model study. Results, particularly from co‐cultured dynamically samples demonstrate excellent bioactivity and vascularized bone forming potential of nanocoating modified 3D bioprinted scaffolds for human bone marrow mesenchymal stem cells and human umbilical vein endothelial cells.

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Biophysical Responses upon the Interaction of Nanomaterials with Cellular Interfaces

Cited by 128 publications

References 57 publications

The performance of gradient alloy quantum dots in cell labeling

The performance of gradient alloy quantum dots in cell labeling

Nanosheet transfection: effective transfer of naked DNA on silica glass

Biologically Inspired Smart Release System Based on 3D Bioprinted Perfused Scaffold for Vascularized Tissue Regeneration

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