Biophysical parameters of rats’ skin after the administration of methimazole

Gołyński, Marcin; Szczepanik, M.; Lutnicki, Krzysztof; Adamek, Ł; Gołyńska, Magdalena; Wilkołek, Piotr; Sitkowski, W; Kurek, Łukasz; Dębiak, Piotr

doi:10.2478/bvip-2014-0049

Cited by 6 publications

(6 citation statements)

References 39 publications

(59 reference statements)

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“…After this period, the rats were fed a commercial diet for laboratory animals (Agropol, Poland). Rats from the control group had access to tap water ad libitum , and the rats from experimental groups were given a 0.05% methimazole (Sigma-Aldrich, USA) water solution, administered ad libitum and known to be a dose that was very effective at inducing hypothyroidism (10). A fresh solution was prepared daily.…”

Section: Methodsmentioning

confidence: 99%

Pituitary-testicular axis dysfunction in methimazole-induced hypothyroidism in rats

Gołyński

Metyk

Szkodziak

et al. 2019

Journal of Veterinary Research

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Introduction: Thyroid hormones play a major role in the regulation of testicular maturation and growth and in the control of Sertoli and Leydig cell functions in adulthood. When naturally occurring, hypothyroidism causes male hypogonadotropic hypogonadism and Sertoli cell function disorders, but when iatrogenic and methimazole-induced its influence on the pituitary-testicular axis function with respect to Sertoli cells is poorly known. Material and Methods: Male adult Wistar rats (n = 14) were divided into two groups: E – taking methimazole orally for 60 days, and C – control animals. After 60 d, the concentrations in serum of testosterone, follicle-stimulating and luteinising hormones, and inhibins A and B were measured. Testicles were examined morphologically: the apoptotic Sertoli cell percentage (ASC%) and number of these cells functional per tubular mm2 (FSCN/Tmm2) were calculated. Results: In group E, inhibin A was higher while inhibin B was lower than in group C. ASC% was higher and FSCN/Tmm2 lower in group E than in group C. Conclusion: A specific modulation of Sertoli cell function in the course of methimazole-induced hypothyroidism leads to a simultaneous concentration increase in inhibin A and decrease in B. Inhibin A might share responsibility for pituitary-testicular axis dysfunction and hypogonadotropic hypogonadism in this model of hypothyroidism.

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Section: Methodsmentioning

confidence: 99%

Pituitary-testicular axis dysfunction in methimazole-induced hypothyroidism in rats

Gołyński

Metyk

Szkodziak

et al. 2019

Journal of Veterinary Research

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“…The methods in question are also becoming increasingly popular in veterinary medicine, especially in scientific research. Biophysical skin parameters (TEWL, SH, skin pH) have been assessed in dogs, cats, horses and laboratory animals (Matousek and Campbell 2002, Watosn et al 2002, Popiel and Nicpoń 2004, Fluhr et al 2006, Yoshihara et al 2007, Shimada et al 2008, Szczepanik et al 2011, 2012, Gołyński et al 2014. The most commonly applied parameter has been TEWL, and most of the studies have concentrated on dogs.…”

Section: Introductionmentioning

confidence: 99%

Influence of horse breed on transepidermal water loss

Szczepanik¹,

Wilkołek²,

Adamek³

et al. 2016

Polish Journal of Veterinary Sciences

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Non-invasive methods of skin condition assessment include, among others, the evaluation of transepidermal water loss (TEWL). The aim of the study was to examine whether TEWL values depend on horse breed. The study was conducted on four breeds: "Felin" ponies (FP) (n=16), Polish koniks (PK) (n=15), Polish cold-blooded horses (PcbH) (n=11) and Wielkopolska horses (WH) (n=12). It was found that horse breed influences TEWL values. In the neck region, statistically significant differences were found between PK and FP (p=0.006), and PK and WH (p=0.0005). In the lumbar region, there were statistically significant differences between FP and PK (p=0.0009), FP and PcbH (p=0.0016) as well as between PK and WH (p=0.000037), and PcbH and WH (p=0.0006). In the inguinal region statistically significant differences were found between FP and PK (p=0.0003), FP and PcbH (p=0.0005), PK and WH (p=0.009) and PcbH and WH (p=0.006). In the lip region statistically significant differences were observed between FP and PK (p=0.013) as well as between PK and PcbH (p=0.029) and PK and WH (p=0.009). In the examination of TEWL animal breed should be taken into consideration. The non-significant differences found in three of the examined body regions may suggest that these regions are the most adequate for TEWL assessment.

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“…The pH of the rat skin is normally ~5-6, 37 and we observed that the skin pH did not change upon massage with the samples. Therefore, when the samples were massaged into the rat skin, they were actually exposed to the pH 5-6 environment, and the sustained release of retinal from PRN similar to that observed in the in vitro release experiment (at pH 5) probably took place.…”

Section: Tolerance Profiles and Histopathology In Skin Of Ratsmentioning

confidence: 71%

Proretinal nanoparticles: stability, release, efficacy, and irritation

Pisetpackdeekul¹,

Supmuang

Pan-In³

et al. 2016

IJN

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Despite many potent biological activities, retinoids such as retinoic acid (RA) and retinal possess dose-related broad side effects. In this study, we show that this problem, which has been unsolvable for a long time, can be tackled through a controlled release strategy in which retinal is continuously delivered to the skin via sustained release from proretinal nanoparticles. The water dispersible proretinal nanoparticles are stable when kept in water at neutral pH and at room temperature for 8 months under light-proof conditions, and show sustained release of retinal into human synthetic sebum at a pH of 5. In the daily topical application tests performed for 4 weeks on rats’ skin, the nanoparticles showed superior ability to increase epidermal thickness compared to RA and retinal, with no skin irritation observed for the proretinal particles, but severe skin irritation observed for RA and free retinal. When tested under occlusion conditions in human volunteers, insignificant skin irritation was observed for the proretinal nanoparticles. The 12-week, double-blind, split-face study on human volunteers indicates better antiaging efficacy of the particles as compared to the free RA.

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Biophysical parameters of rats’ skin after the administration of methimazole

Cited by 6 publications

References 39 publications

Pituitary-testicular axis dysfunction in methimazole-induced hypothyroidism in rats

Pituitary-testicular axis dysfunction in methimazole-induced hypothyroidism in rats

Influence of horse breed on transepidermal water loss

Proretinal nanoparticles: stability, release, efficacy, and irritation

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