Biophysical, molecular dynamics and cellular studies on the interaction of nickel oxide nanoparticles with tau proteins and neuron-like cells

Hosseinali, Sara Haji; Boushehri, Zahra Pourmokhtar; Rasti, Behnam; Mirpour, Mirsasan; Shahpasand, Koorosh; Falahati, Mojtaba

doi:10.1016/j.ijbiomac.2018.12.062

Cited by 17 publications

(4 citation statements)

References 37 publications

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“…Second, the two size forms of Ni NPs were characterized by different critical variable parameters, with the exception, in general, of the effect on the brain and behavioral responses. The latter is consistent with the manifestation of neurotoxicity in Ni-containing NPs in vitro [33] and in vivo [34], which depends on the size and composition of particles. At the same time, some signs of increased anxiety in animals were revealed in the case of the soluble salt form of Ni administration in contrast to NPs.…”

Section: Discussionsupporting

confidence: 86%

“…The reasons for the specific effects of nanotoxicity of metallic Ni particles should be discussed in light of the processes occurring on the surface of Ni NPs during their interaction with the biological environment, including the catalytic generation of ROS [32,35], as well as the formation of adsorption layers ("crowns") of biopolymer molecules [33]. A certain role in Ni NPs' action in vivo can also be played by various mechanisms of transport of these NPs through biological barriers, such as clathrin-dependent endocytosis and persorption [36].…”

Section: Discussionmentioning

confidence: 99%

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Peroral Toxicological Assessment of Two-Dimensional Forms of Nickel Nanoparticles Sized between 20 and 120 nm

et al. 2022

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Nickel (Ni) nanoparticles (NPs) are used as technological aids–catalysts in the oil and fat industry, in pharmaceuticals, and in the production of cosmetics and pesticides. The acute and subchronic oral toxicity of metallic Ni in the nanoform is not well understood. The study aimed to investigate the acute and subchronic oral toxicity of Ni NPs to rats. We used two NP preparations (Ni NP1 and Ni NP2) with spherical particles and an average diameter of 53.7 and 70.9 nm according to the electron microscopy data. In the study of acute toxicity, both kinds of Ni NPs were administered to male and female Wistar rats aged 8 weeks as a single dose of 2000 mg/kg b.w. through a gastric gavage. In the subchronic experiment, male Wistar rats initially aged 7 weeks received for 92 days Ni NP1 and Ni NP2 as well as the “traditional” soluble salt form of Ni (Ni basic carbonate) at doses of 0.1, 1, and 10 mg/kg body weight (mg/kg b.w.) in terms of Ni content as a part of the diet consumed. As a result, in an acute study, the oral LD50 for Ni NP2 in male and female rats was about 1600 mg/kg b.w. (IV hazard class). The oral dose of Ni NP1 equal to 2000 mg/kg b.w. exceeded LD100 for males and corresponded to LD90 for females. In the subchronic study, the bioaccumulation of both Ni NPs as well as Ni salt was observed in the kidney but not in the liver and spleen. Ni NP1 decreased body weight only at a dose of 1 mg/kg b.w.; affected the relative weight of the spleen at 0.1 mg/kg, the brain at 1.0 mg/kg, and the thymus at 10 mg/kg; and decreased locomotor activity at 0.1 and 10 mg/kg. Thus, for Ni NP1, in such cases where a monotonic dose–response relationship could be traced, LOEL could be stated at 10 mg/kg b.w./day for 92 days of oral intake. However, for some endpoints where such a monotonic relationship could be absent, significant toxic effects were observed even at a dose 0.1 mg/kg. In the case of Ni NP2, changes in the relative weight of the liver, thymus, and brain were recorded starting from 0.1 mg/kg b.w.; locomotor activity decreased starting from 0.1 mg/kg. Other effects, including basophiles count and platelet system indexes, were observed at a dose of 1 mg/kg or higher. Thus, the LOEL value for Ni NP2 can be fixed at 0.1 mg/kg. The critical organs affected by both Ni NPs were the brain and immune system. Most of the toxic effects exhibited by metallic Ni NPs were absent or had an opposite orientation upon administration of equivalent doses of Ni in the salt form which indicates the signs of “nanotoxicity” in metallic Ni NPs. In conclusion, the data obtained show that there may be some additional health risks caused by the intake of Ni in a nanoform compared to soluble ionized forms of this element at equivalent doses.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Peroral Toxicological Assessment of Two-Dimensional Forms of Nickel Nanoparticles Sized between 20 and 120 nm

et al. 2022

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“…In vitro viability assays are used to measure the proportion of living cells after a potentially traumatic procedure; they are dependent on the alteration of the permeability of the membrane that is determined by the incorporation of agents that are normally impermeable, [20]; for instance, the trypan blue method is based on a blue dye which stains the dead cells due to the damage to the membrane permeability.…”

Section: Introductionmentioning

confidence: 99%

Polyethylene Glycol-Coated Gold Nanoparticles as DNA and Atorvastatin Delivery Systems and Cytotoxicity Evaluation

Zamora-Justo

Abrica-González

Vázquez-Martínez

et al. 2019

Journal of Nanomaterials

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The application of nanoscience and nanotechnology in medicine has been useful in the diagnosis, monitoring, and treatment of many diseases. Gold nanoparticles are commonly used for medical imaging studies, biosensors, drug delivery systems, and gene therapy. It has been reported that nanoparticles coated with specific polymers improve the biocompatibility and stability and decrease the cytotoxicity of the nanoparticles. In this work, we performed transfection studies of gold nanoparticles coated with polyethylene glycol, synthetized by two different methods, in a human embryonic kidney cell culture (HEK 293), by using plasmids pSV-β-Gal and pIRES2-EGFP. In addition, we also evaluated the cell uptake of a fluorescent drug (atorvastatin) using the synthetized gold nanoparticles as carriers. Furthermore, the study of cell viability after the interaction between these cells and the nanoparticles was performed. It was shown that the polyethylene glycol-coated gold nanoparticles presented transfection efficiency and cell uptake greater than 45% in each case. These results suggest that the synthetized gold nanoparticles coated with polyethylene glycol could be used successfully and safely as DNA and drug delivery systems.

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“…As a result, there was expression of apoptosis gene Bax and in increase in Bax/Bcl-2 ratio, greater lactate dehydrogenase and caspases 3 and 9 activity. In further research [52] it was shown that exposure to NiO NPs in high doses induced oxidation stress and apoptosis of SH-SY5Y cells whereas in case doses were non-lethal, there was prevailing interaction between nanomaterial and tau-protein structures together with an increase in hydrophobic tau and formation of its amorphous aggregates.…”

Section: Cytotoxicity Of Ni-containing Nanomaterialsmentioning

confidence: 99%

Assessing risks caused by nickel-based nanomaterials: hazardous factor identification

Gmoshinski¹,

lane²,

Хотимченко³

2021

Health Risk Analysis

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Nanoparticles of nickel (Ni) and its compounds attract a lot of attention bearing in mind their promising innovative properties allowing their use as catalysts, components in electrical appliances, electronic devices and photonic appliances, and materials used in producing medications, diagnostic preparations, and pesticides. Production volumes of these materials in their nano-form are likely to grow rapidly in the nearest future and it involves greater loads created by these nanomaterials on a human body. And we should remember that Ni and its compounds are highly toxic for humans even in their traditional disperse forms. Their toxicity induces oxidative stress, cellular membranes and mitochondria dysfunction, expression of nuclear transcription factors that are responsible for apoptosis, caspases, as well as proto-oncogenes. Leading role in toxicity of Ni-containing nanomaterials obviously belongs to ions of heavy Ni++ being emitted from them since this heavy metal has pro-oxidant properties and influences enzyme activity and gene expression. Cytotoxic effects produced by Ni-containing nanomaterials were revealed in Model experiments in vitro performed with suing cellular cultures that were morphologically and functionally similar to epithelial cells of respiratory and gastrointestinal tract, liver, kidneys, and nervous system; these materials were able to stimulate oxidant stress, influence expression of apoptosis proteins and nuclear transcription factors, induce apoptosis and necrosis. There are data indicating that Ni-containing nanomaterials can produce malignant transforming effects in vitro. All the above mentioned proves that nickel compounds in their nanoform are a new hazardous factor that requires assessing related risks for workers, consumer, and population in general. Our review focuses on analyzing literature sources on cytotoxicity of Ni-containing nanomaterials and their effects produced on molecular-genetic and cellular levels taken over a period starting from 2011.

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Biophysical, molecular dynamics and cellular studies on the interaction of nickel oxide nanoparticles with tau proteins and neuron-like cells

Cited by 17 publications

References 37 publications

Peroral Toxicological Assessment of Two-Dimensional Forms of Nickel Nanoparticles Sized between 20 and 120 nm

Peroral Toxicological Assessment of Two-Dimensional Forms of Nickel Nanoparticles Sized between 20 and 120 nm

Polyethylene Glycol-Coated Gold Nanoparticles as DNA and Atorvastatin Delivery Systems and Cytotoxicity Evaluation

Assessing risks caused by nickel-based nanomaterials: hazardous factor identification

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