Biophysical methods for biochip analysis. Use of wide-field digital fluorescence microscopy

Barsky, V. E.; Yegorov, E. E.; Kreindlin, E. I.; Lysov, Yu. P.; Pan’kov, S. V.; Urasov, D. A.; Urasov, R. A.; Заседателев, А. С.

doi:10.1134/s0006350912030049

Cited by 2 publications

(1 citation statement)

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“…Another type of microarray analyzer, one based on wide-field digital microscopy, utilizes a defocused laser beam to excite the fluorescence of all objects located in the analyzer's field of view (FOV), and an image of fluorescence microarray cells is obtained using an image detector (CCD or CMOS camera) [20,21]. The size of the analyzed microarray area is determined by the objective-ocular FOV and may vary from several square microns to hundreds of square millimeters.…”

Section: Introductionmentioning

confidence: 99%

Microarray analyzer based on wide field fluorescent microscopy with laser illumination and a device for speckle suppression

Lysov

Barsky

Urasov

et al. 2017

Biomed. Opt. Express

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A microarray analyzer was developed to obtain images and measure the fluorescence intensity of microarrays at three wavelengths from 380 nm to 850 nm. The analyzer contains lasers to excite fluorescence, barrier filters, optics to project images on an image detector, and a device for suppressing laser speckles on the microarray support. The speckle suppression device contains a fibre-optic bundle and a rotating mirror positioned in a way to change the distance between the bundle butt and mirror surface during each mirror revolution. The analyzer provides for measurements with accuracy within ± 5%. Obtaining images at several exposure times allowed a significant expansion in the range of measured fluorescence intensities. The analyzer is useful for high throughput analysis of the same type of microarrays.

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Section: Introductionmentioning

confidence: 99%