Biophysical Investigation of RNA ⋅ DNA : DNA Triple Helix and RNA : DNA Heteroduplex Formation by the lncRNAs MEG3 and Fendrr
Nina M. Krause,
Jasleen K. Bains,
Julius Blechar
et al.
Abstract:Long non‐coding RNAs (lncRNAs) are important regulators of gene expression and can associate with DNA as RNA:DNA heteroduplexes or RNA•DNA:DNA triple helix structures. Here, we review in vitro biochemical and biophysical experiments including electromobility shift assays (EMSA), circular dichroism (CD) spectroscopy, thermal melting analysis, microscale thermophoresis (MST), single‐molecule Förster resonance energy transfer (smFRET) and nuclear magnetic resonance (NMR) spectroscopy to investigate RNA•DNA:DNA tr… Show more
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