Biophysical insights into the interaction of two enantiomers of Ru(II) complex [Ru(bpy)2(7-CH3-dppz)]2+ with the RNA poly(U-A⁎U) triplex

Dong, Zhan; Liu, Xiaohua; Tan, Lifeng

doi:10.1007/s00775-020-01825-9

Cited by 6 publications

(2 citation statements)

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“…The method of absorption spectroscopic studies follows that reported in previous literatures. , For the titration analyses of CT-DNA by the enantiomers, the absorbance of DNA itself was eliminated by adding an equal amount of DNA to both the sample solution (20 μ M of Ru complex) and the reference solution (blank buffer), and all the experimental solutions were thoroughly mixed for 5 min before measurements. The spectra were recorded in the wavelength range of 200–600 nm at 298 K. The variation in the concentration of the metal complex solution caused by the additions of DNA was insignificant and ignored. , Each titration experiment was repeated three times, and the titration data were expressed as mean ± standard deviation.…”

Section: Experimental Sectionmentioning

confidence: 99%

“…The above equations, eqs and , can also be applied in absorption spectroscopic titration to calculate the corresponding DNA-binding parameters, except using the absorbance change at a specific DNA concentration and the maximum absorbance change of the fully bound complex with the absorbance of the complex alone as the control instead of ( I - I 0 ) and ( I f - I 0 ) respectively …”

Section: Experimental Sectionmentioning

confidence: 99%

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Comparative Studies on DNA-Binding Mechanisms between Enantiomers of a Polypyridyl Ruthenium(II) Complex

et al. 2022

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A pair of ruthenium(II) complex enantiomers, Δ- and Λ-[Ru(bpy)2MBIP]2+ (bpy = 2,2′-bipyridine, MBIP = 2-(3-bromophenyl)imidazo[5,6-f]phenanthroline), were designed, synthesized, and characterized. Comparative studies between the enantiomers on their binding behaviors to calf thymus DNA (CT-DNA) were conducted using UV−visible, fluorescence, and circular dichroism spectroscopies, viscosity measurements, isothermal titration calorimetry, a photocleavage experiment, and molecular simulation. The experimental results indicated that both the enantiomers spontaneously bound to CT-DNA through intercalation stabilized by the van der Waals force or the hydrogen bond and driven by enthalpy and that Δ-[Ru(bpy)2MBIP]2+ intercalated into DNA more deeply than Λ-[Ru(bpy)2MBIP]2+ did and exhibited a better DNA photocleavage ability. Molecular simulation further indicated that Δ-[Ru(bpy)2MBIP]2+ more preferentially intercalated between the base pairs of CT-DNA to the major groove, and Λ-[Ru(bpy)2MBIP]2+ more favorably intercalated to the minor groove. These research findings should be very helpful to the understanding of the stereoselectivity mechanism of DNA-bindings of metal complexes, and be useful for the design of novel metal-complex-based antitumor drugs with higher efficacy and lower toxicity.

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