Biophysical insights from supported lipid patches

Miller, Ethan J.; Stubbington, Liam; Dinet, Céline; Staykova, Margarita

doi:10.1016/bs.abl.2019.01.004

Cited by 2 publications

(5 citation statements)

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“…The fusion of lipid vesicles has been explored in detail for a range of substrates. 22 , 23 , 41 − 43 We find that while the fusion of PGUVs proceeds in a similar fashion, there are some important differences. LGUVs fused instantaneously on hydrophilic glass and PDMS substrates using iso-osmotic buffer.…”

Section: Results and Discussionmentioning

confidence: 76%

“…We first explored the optimal conditions for fusing polymeric GUVs (PGUV) on glass and PDMS and compared them to the fusion of lipid GUVs (LGUV). The fusion of lipid vesicles has been explored in detail for a range of substrates. ,,− We find that while the fusion of PGUVs proceeds in a similar fashion, there are some important differences. LGUVs fused instantaneously on hydrophilic glass and PDMS substrates using iso-osmotic buffer.…”

Section: Results and Discussionmentioning

confidence: 99%

“…Like lipid GUVs, PGUVs were able to fuse to hydrophilic glass and PDMS substrates and form patches with distinct heart or circular shapes (Figure ). The effective vesicle–substrate interaction, which is the sum of the van der Waals attraction, the hydration repulsion, and the entropic repulsion arising from thermal membrane undulations, is sufficient to increase the membrane tension above a critical value leading to vesicle rupture and fusion. ,,,, However, polymer vesicles needed some additional adjustments to fuse to glass and PDMS. These included (1) baking the plasma-oxidized glass at 200 °C to reduce the hydration repulsion force or (2) subjecting the vesicles to hypo-osmotic shock, which swells them and increases their membrane tension, as well as reducing the undulation repulsion with the substrate .…”

Section: Discussionmentioning

confidence: 99%

“…15 In this work, we compared the formation and properties of supported membrane patches of PEO14-PBD22 and phospholipids. 22 We characterized the fluidity and integrity of the patch membrane and the properties of its edges. We used the knowledge learned from patches to determine the conditions for forming continuous polymer membranes.…”

Section: ■ Introductionmentioning

confidence: 99%

“…In this work, we compared the formation and properties of supported membrane patches of PEO14-PBD22 and phospholipids . We characterized the fluidity and integrity of the patch membrane and the properties of its edges.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Study of Lipid- and Polymer-Supported Membranes Obtained by Vesicle Fusion

2022

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We compare the fusion of giant lipid and block-copolymer vesicles on glass and poly(dimethylsiloxane) substrates. Both types of vesicles are similar in their ability to fuse to hydrophilic substrates and form patches with distinct heart or circular shapes. We use epifluorescence/confocal microscopy and atomic force microscopy on membrane patches to (i) characterize bilayer fluidity and patch-edge stability and (ii) follow the intermediate stages in the formation of continuous supported bilayers. Polymer membranes show much lower membrane fluidity and, unlike lipids, an inability of adjacent patches to fuse spontaneously into continuous membranes. We ascribe this effect to hydration repulsion forces acting between the patch edges, which can be diminished by increasing the sample temperature. We show that large areas of supported polymer membranes can be created by fusing giant vesicles on glass or poly(dimethylsiloxane) substrates and annealing their edges.

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